pLV3-CMV-TESC-3×FLAG-CopGFP-Pu

pLV3-CMV-TESC-3×FLAG-CopGFP-Puro plasmid

PVT96846

pLV3-CMV-TESC-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TESC(human)-3×FLAG-CopGFP-Puro
Alias: NM_017899.4;CHP3; TSC
Gene lenght: 642bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TESC-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TESC-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TESC-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcgctgccca ctccgcgtct 2341 gaggaggtgc gggagctcga gggcaagacc ggcttctcat cggatcagat cgagcagctc 2401 catcggagat ttaagcagct gagtggagat cagcctacca ttcgcaagga gaacttcaac 2461 aatgtcccgg acctggagct caaccccatc cgatccaaaa ttgttcgtgc cttcttcgac 2521 aacaggaacc tgcgcaaggg acccagtggc ctggctgatg agatcaattt cgaggacttc 2581 ctgaccatca tgtcctactt ccggcccatc gacaccacca tggacgagga acaggtggag 2641 ctgtcccgga aggagaagct gagatttctg ttccacatgt acgactcgga cagcgacggc 2701 cgcatcactc tggaagaata tcgaaatgtg gtcgaggagc tgctgtcggg aaaccctcac 2761 atcgagaagg agtccgctcg ctccatcgcc gacggggcca tgatggaggc ggccagcgtg 2821 tgcatggggc agatggagcc tgatcaggtg tacgagggga tcaccttcga ggacttcctg 2881 aagatctggc aggggatcga cattgagacc aagatgcacg tccgcttcct taacatggaa 2941 accatggccc tctgccacgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3001 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3061 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3121 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3181 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3241 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3301 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3361 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3421 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3481 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3541 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 3601 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3661 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3721 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3781 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3841 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3901 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 3961 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4021 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4081 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4141 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4201 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4261 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4321 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4381 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4441 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4501 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4561 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 4621 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 4681 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 4741 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 4801 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 4861 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 4921 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 4981 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5041 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5101 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5161 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5221 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5281 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5341 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5401 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5461 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5521 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 5581 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 5641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 5701 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 5881 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 5941 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6121 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6241 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6301 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8461 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8521 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8581 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8641 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8701 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8761 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8821 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8881 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.