pCMV-DCBLD2-3×FLAG-Puro plasmi

pCMV-DCBLD2-3×FLAG-Puro plasmid

PVT96804

pCMV-DCBLD2-3×FLAG-Puro plasmid Information

Plasmid name: pCMV-DCBLD2(human)-3×FLAG-Puro
Alias: NM_080927.4;CLCP1; ESDN
Gene lenght: 2325bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-DCBLD2-3×FLAG-Puro plasmid Description

pCMV-DCBLD2-3×FLAG-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-DCBLD2-3×FLAG-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCGC CACCatggcg agccgggcgg tggtgagagc caggcgctgc 961 ccgcagtgtc cccaagtccg ggccgcggcc gccgcccccg cctgggccgc gctccccctc 1021 tcccgctccc tccctccctg ctccaactcc tcctccttct ccatgcctct gttcctcctg 1081 ctcttacttg tcctgctcct gctgctcgag gacgctggag cccagcaagg tgatggatgt 1141 ggacacactg tactaggccc tgagagtgga acccttacat ccataaacta cccacagacc 1201 tatcccaaca gcactgtttg tgaatgggag atccgtgtaa agatgggaga gagagttcgc 1261 atcaaatttg gtgactttga cattgaagat tctgattctt gtcactttaa ttacttgaga 1321 atttataatg gaattggagt cagcagaact gaaataggca aatactgtgg tctggggttg 1381 caaatgaacc attcaattga atcaaaaggc aatgaaatca cattgctgtt catgagtgga 1441 atccatgttt ctggacgcgg atttttggcc tcatactctg ttatagataa acaagatcta 1501 attacttgtt tggacactgc atccaatttt ttggaacctg agttcagtaa gtactgccca 1561 gctggttgtc tgcttccttt tgctgagata tctggaacaa ttcctcatgg atatagagat 1621 tcctcgccat tgtgcatggc tggtgtgcat gcaggagtag tgtcaaacac gttgggcggc 1681 caaatcagtg ttgtaattag taaaggtatc ccctattatg aaagttcttt ggctaacaac 1741 gtcacatctg tggtgggaca cttatctaca agtcttttta catttaagac aagtggatgt 1801 tatggaacac tggggatgga gtctggtgtg atcgcggatc ctcaaataac agcatcatct 1861 gtgctggagt ggactgacca cacagggcaa gagaacagtt ggaaacccaa aaaagccagg 1921 ctgaaaaaac ctggaccgcc ttgggctgct tttgccactg atgaatacca gtggttacaa 1981 atagatttga ataaggaaaa gaaaataaca ggcattataa ccactggatc caccatggtg 2041 gagcacaatt actatgtgtc tgcctacaga atcctgtaca gtgatgatgg gcagaaatgg 2101 actgtgtaca gagagcctgg tgtggagcaa gataagatat ttcaaggaaa caaagattat 2161 caccaggatg tgcgtaataa ctttttgcca ccaattattg cacgttttat tagagtgaat 2221 cctacccaat ggcagcagaa aattgccatg aaaatggagc tgctcggatg tcagtttatt 2281 cctaaaggtc gtcctccaaa acttactcaa cctccacctc ctcggaacag caatgacctc 2341 aaaaacacta cagcccctcc aaaaatagcc aaaggtcgtg ccccaaaatt tacgcaacca 2401 ctacaacctc gcagtagcaa tgaatttcct gcacagacag aacaaacaac tgccagtcct 2461 gatatcagaa atactaccgt aactccaaat gtaaccaaag atgtagcgct ggctgcagtt 2521 cttgtccctg tgctggtcat ggtcctcact actctcattc tcatattagt gtgtgcttgg 2581 cactggagaa acagaaagaa aaaaactgaa ggcacctatg acttacctta ctgggaccgg 2641 gcaggttggt ggaaaggaat gaagcagttt cttcctgcaa aagcagtgga ccatgaggaa 2701 accccagttc gctatagcag cagcgaagtt aatcacctga gtccaagaga agtcaccaca 2761 gtgctgcagg ctgactctgc agagtatgct cagccactgg taggaggaat tgttggtaca 2821 cttcatcaaa gatctacctt taaaccagaa gaaggaaaag aagcaggcta tgcagaccta 2881 gatccttaca actcaccagg gcaggaagtt tatcatgcct atgctgaacc actcccaatt 2941 acggggcctg agtatgcaac cccaatcatc atggacatgt cagggcaccc cacaacttca 3001 gttggtcagc cctccacatc cactttcaag gctacgggga accaacctcc cccactagtg 3061 ggaacttaca atacacttct ctccaggact gacagctgct cctcagccca ggcccagtat 3121 gataccccga aagctgggaa gccaggtcta cctgccccag acgaattggt gtaccaggtg 3181 ccacagagca cacaagaagt atcaggagca ggaagggatg gggaatgtga tgtttttaaa 3241 gaaatccttC TCGAGACCGG TGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3301 ATCGATTACA AGGATGACGA TGACAAGTGA CCGGTaAGTT TAAACCGCTG ATCAGCCTCG 3361 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC 3421 CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT 3481 CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT 3541 TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA 3601 AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG 3661 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 3721 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 3781 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 3841 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 3901 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 3961 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGGCCATTTC GGCCTATTGG 4021 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC 4081 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4141 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 4201 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 4261 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 4321 ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 4381 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC Cgggatgacc gagtacaagc ccacggtgcg 4441 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 4501 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 4561 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 4621 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 4681 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 4741 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 4801 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 4861 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 4921 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 4981 cacctggtgc atgacccgca agcccggtgc ctgaTTCGAA ATGACCGACC AAGCGACGCC 5041 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG 5101 AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT 5161 CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 5221 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 5281 CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC 5341 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 5401 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 5461 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 5521 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 5581 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 5641 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 5701 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 5761 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 5821 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 5881 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 5941 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 6001 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 6061 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 6121 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 6181 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 6241 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA 6301 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 6361 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 6421 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 6481 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 6541 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 6601 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 6661 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 6721 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 6781 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 6841 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 6901 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 6961 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 7021 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 7081 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 7141 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 7201 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 7261 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 7321 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 7381 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 7441 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.