pT7-6×His-MBP-DDX17 plasmid

pT7-6×His-MBP-DDX17 plasmid

PVT96788

pT7-6×His-MBP-DDX17 plasmid Information

Plasmid name: pT7-6×His-MBP-DDX17(human)
Alias: NM_006386.5;P72; RH70
Gene lenght: 2187bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: T7ter
Reverse primer:
Copy number: Low
Induction:

pT7-6×His-MBP-DDX17 plasmid Description

pT7-6×His-MBP-DDX17 is a plasmid suitable for protein expression in Escherichia coli.

pT7-6×His-MBP-DDX17 plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 5101 agcagcggcc tggtgccgcg cggcagccat atgaaaatcg aagaaggtaa actggtaatc 5161 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa 5221 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag 5281 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc 5341 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg 5401 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct 5461 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg 5521 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc 5581 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc 5641 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa 5701 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat 5761 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg 5821 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc 5881 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc 5941 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt 6001 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa 6061 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc 6121 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac 6181 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac taattcgagc 6241 tcgaacaaca acaacaataa caataacaac aacctcgggg agaatctcta cttccaggga 6301 ggatccccca ccggctttgt agccccgatt ctctgtgttt tgctcccgtc tccgacgaga 6361 gaggcggcga cggtggcgtc tgcgacggga gacagcgcgt cggagcgaga gagcgctgcg 6421 cctgccgccg ccccaacagc ggaggcgccg ccgccatcgg tcgtcaccag accggagccg 6481 caggccctcc cgagcccggc catccgtgcc ccgctcccag atctctatcc ttttgggacc 6541 atgcgcggag gaggctttgg ggaccgggac cgggatcgtg accgtggagg atttggagca 6601 agaggtggtg gtggccttcc cccgaagaaa tttggtaatc ctggggagcg tttgcgtaaa 6661 aaaaagtggg atttgagtga gctccccaag tttgagaaaa atttttatgt ggaacatccg 6721 gaagtagcaa ggctgacacc atatgaggtt gatgagctac gccgaaagaa ggagattaca 6781 gtgagggggg gagatgtttg tcctaaaccc gtgtttgcct tccatcatgc taacttccca 6841 caatatgtaa tggatgtgtt gatggatcag cactttacag aaccaactcc aattcagtgc 6901 cagggatttc cgttggctct tagtggccgg gatatggtgg gcattgctca gactggctct 6961 gggaagacgt tggcgtatct cctgcctgca attgttcata ttaaccacca gccatacttg 7021 gaaaggggag atggcccaat ctgtctagtt ctggctccta ccagagagct tgcccagcaa 7081 gtacagcagg tggccgatga ctatggcaaa tgttctagat tgaagagtac ttgtatttat 7141 ggaggtgctc ctaaaggtcc ccagattcga gacttggaaa gaggtgttga gatctgcata 7201 gccactcctg gacgtctgat agatttcctg gagtcaggaa agacaaatct tcgccgatgt 7261 acttaccttg tattggacga agctgacaga atgcttgata tggggtttga accccagatc 7321 cgtaaaattg ttgaccaaat caggcctgat aggcagacac tgatgtggag tgcaacctgg 7381 ccaaaagaag taagacagct tgcagaggat ttccttcgtg attacaccca gatcaacgta 7441 ggcaatctgg agttgagtgc caaccacaac atcctccaga tagtggatgt ctgcatggaa 7501 agtgaaaaag accacaagtt gatccaacta atggaagaaa taatggctga aaaggaaaac 7561 aaaacaataa tatttgtgga gacaaagaga cgctgtgatg atctgactcg aaggatgcgc 7621 agagatggtt ggccagctat gtgtatccat ggagacaaga gtcaaccaga aagagattgg 7681 gtacttaatg agttccgttc tggaaaggca cccatcctta ttgctacaga tgtagcctcc 7741 cgtgggctag atgtggaaga tgtcaagttt gtgatcaact atgactatcc aaacagctca 7801 gaggattatg tgcaccgtat tggccgaaca gcccgtagca ccaacaaggg taccgcctat 7861 accttcttca ccccagggaa cctaaaacag gccagagagc ttatcaaagt gctggaagag 7921 gccaatcagg ctatcaatcc aaaactgatg cagcttgtgg accacagagg aggcggcgga 7981 ggcgggggtg gtcgttctcg ttaccggacc acttcttcag ccaacaatcc caatctgatg 8041 tatcaggatg agtgtgaccg aaggcttcga ggagtcaagg atggtggccg gagagactct 8101 gcaagctatc gggatcgtag tgaaaccgat agagctggtt atgctaatgg cagtggctat 8161 ggaagtccaa attctgcctt tggagcacaa gcaggccaat acacctatgg tcaaggcacc 8221 tatggggcag ctgcttatgg caccagtagc tatacagctc aagaatatgg tgctggcact 8281 tatggagcta gtagcaccac ctcaactggg agaagttcac agagctctag ccagcagttt 8341 agtgggatag gccggtctgg gcagcagcca cagccactga tgtcacaaca gtttgcacag 8401 cctccgggag ctaccaatat gataggttac atggggcaga ctgcctacca ataccctcct 8461 cctcctcccc ctcctcctcc ttcacgtaaa TAActcgagt gagatccggc tgctaacaaa 8521 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt 8581 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.