pCMV-mCherry-RAB11A-Neo plasmi

pCMV-mCherry-RAB11A-Neo plasmid

PVT96778

pCMV-mCherry-RAB11A-Neo plasmid Information

Plasmid name: pCMV-mCherry-RAB11A(human)-Neo
Alias: NM_004663.5;YL8
Gene lenght: 651bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-RAB11A-Neo plasmid Description

pCMV-mCherry-RAB11A-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-RAB11A-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tcaagcttcg aattccggtg gaggcggatc aggaggcggt 1381 ggatctatgg gcacccgcga cgacgagtac gactacctct ttaaagttgt ccttattgga 1441 gattctggtg ttggaaagag taatctcctg tctcgattta ctcgaaatga gtttaatctg 1501 gaaagcaaga gcaccattgg agtagagttt gcaacaagaa gcatccaggt tgatggaaaa 1561 acaataaagg cacagatatg ggacacagca gggcaagagc gatatcgagc tataacatca 1621 gcatattatc gtggagctgt aggtgcctta ttggtttatg acattgctaa acatctcaca 1681 tatgaaaatg tagagcgatg gctgaaagaa ctgagagatc atgctgatag taacattgtt 1741 atcatgcttg tgggcaataa gagtgatcta cgtcatctca gggcagttcc tacagatgaa 1801 gcaagagctt ttgcagaaaa gaatggtttg tcattcattg aaacttcggc cctagactct 1861 acaaatgtag aagctgcttt tcagacaatt ttaacagaga tttaccgcat tgtttctcag 1921 aagcaaatgt cagacagacg cgaaaatgac atgtctccaa gcaacaatgt ggttcctatt 1981 catgttccac caaccactga aaacaagcca aaggtgcagt gctgtcagaa catctaagga 2041 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt 2101 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 2161 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 2221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 2281 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 2341 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 2401 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 2461 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 2521 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 2581 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 2641 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 2701 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 2761 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 2821 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 2881 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 2941 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 3001 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3061 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 3121 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 3181 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 3241 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 3301 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 3361 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 3421 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 3481 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 3541 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 3601 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 3661 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 3721 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 3781 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 3841 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 3901 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 3961 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 4021 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 4081 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 4141 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 4201 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 4261 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 4321 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 4381 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 4441 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 4501 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 4561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 4621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 4681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 4741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 4801 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 4861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 4921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 4981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 5041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 5101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 5161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 5221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 5281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 5341 tcccctgatt ctgtggataa ccgtattacc gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.