pCMV-EGFP-SLC5A1-SV40PolyA-Neo

pCMV-EGFP-SLC5A1-SV40PolyA-Neo plasmid

PVT96772

pCMV-EGFP-SLC5A1-SV40PolyA-Neo plasmid Information

Plasmid name: pCMV-EGFP-SLC5A1(human)-SV40PolyA-Neo
Alias: NM_000343.4;D22S675; NAGT; SGLT-1; SGLT1
Gene lenght: 1995bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-SLC5A1-SV40PolyA-Neo plasmid Description

pCMV-EGFP-SLC5A1-SV40PolyA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-SLC5A1-SV40PolyA-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggt ggaggcggat caggaggcgg tggatctatg 1381 gacagtagca cctggagccc caagaccacc gcggtcaccc ggcctgttga gacccacgag 1441 ctcattcgca atgcagccga tatctccatc atcgttatct acttcgtggt agtgatggcc 1501 gtcggactgt gggctatgtt ttccaccaat cgtgggactg ttggaggctt cttcctggca 1561 ggccgaagta tggtgtggtg gccgattgga gcctccctct ttgctagtaa cattggaagt 1621 ggccactttg tggggctggc cgggactggg gcagcttcag gcatcgccat tggaggcttt 1681 gaatggaatg ccctggtttt ggtggttgtg ctgggctggc tgtttgtccc catctatatt 1741 aaggctgggg tggtgacaat gccagagtac ctgaggaagc ggtttggagg ccagcggatc 1801 caggtctacc tttcccttct gtccctgctg ctctacattt tcaccaagat ctcggcagac 1861 atcttctcgg gggccatatt catcaatctg gccttaggcc tgaatctgta tttagccatc 1921 tttctcttat tggcaatcac tgccctttac acaattacag ggggcctggc ggcggtgatt 1981 tacacggaca ccttgcagac ggtgatcatg ctggtggggt ctttaatcct gactgggttt 2041 gcttttcacg aagtgggagg ctatgacgcc ttcatggaaa agtacatgaa agccattcca 2101 accatagtgt ctgatggcaa caccaccttt caggaaaaat gctacactcc aagggccgac 2161 tccttccaca tcttccgaga tcccctcacg ggagacctcc catggcctgg gttcatcttt 2221 gggatgtcca tccttacctt gtggtactgg tgcacagatc aggtcattgt gcagcgctgc 2281 ctctcagcca agaatatgtc tcacgtgaag ggtggctgca tcctgtgtgg gtatctaaag 2341 ctgatgccca tgttcatcat ggtgatgcca ggaatgatca gccgcattct gtacacagaa 2401 aaaattgcct gtgtcgtccc ttcagaatgt gagaaatatt gcggtaccaa ggttggctgt 2461 accaacatcg cctatccaac cttagtggtg gagctcatgc ccaatggact gcgaggcctg 2521 atgctatcag tcatgctggc ctccctcatg agctccctga cctccatctt caacagcgcc 2581 agcaccctct tcaccatgga catctacgcc aaggtccgca agagagcatc tgagaaagag 2641 ctcatgattg ccggaaggtt gtttatcctg gtgctgattg gcatcagcat cgcctgggtg 2701 cccattgtgc agtcagcaca aagtgggcaa ctcttcgatt acatccagtc catcaccagt 2761 tacttgggac cacccattgc ggctgtcttc ctgcttgcta ttttctggaa gagagtcaat 2821 gagccaggag ccttttgggg actgatccta ggacttctga ttgggatttc acgtatgatt 2881 actgagtttg cttatggaac cgggagctgc atggagccca gcaactgtcc cacgattatc 2941 tgtggggtgc actacttgta ctttgccatt atcctcttcg ccatttcttt catcaccatc 3001 gtggtcatct ccctcctcac caaacccatt ccggatgtgc atctctaccg tctgtgttgg 3061 agcctgcgca acagcaaaga ggagcgtatt gacctggatg cggaagagga gaacatccaa 3121 gaaggcccta aggagaccat tgaaatagaa acacaagttc ctgagaagaa aaaaggaatc 3181 ttcaggagag cctatgacct attttgtggg ctagagcagc acggtgcacc caagatgact 3241 gaggaagagg agaaagccat gaagatgaag atgacggaca cctctgagaa gcctttgtgg 3301 aggacagtgt tgaacgtcaa tggcatcatc ctggtgaccg tggctgtctt ttgccatgca 3361 tattttgcct gagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag 3421 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 3481 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 3541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3601 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt 3661 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 3721 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 3781 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 3841 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 3901 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 3961 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 4021 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 4081 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg 4141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa 4261 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4381 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4441 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4501 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 4561 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga 4621 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 4681 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 4741 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 4801 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg 4861 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 4921 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 4981 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 5041 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 5101 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 5161 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 5221 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 5281 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 5341 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 5401 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 5461 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 5521 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 5581 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac 5641 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt 5701 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc 5761 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc 5821 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc 5881 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 5941 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 6001 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 6061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 6121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 6181 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 6241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 6301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 6361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 6421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 6481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 6541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 6601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 6661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 6721 ggataaccgt attaccgcca tgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.