pCMV-mCherry-RAB7A-Neo plasmid

pCMV-mCherry-RAB7A-Neo plasmid

PVT96679

pCMV-mCherry-RAB7A-Neo plasmid Information

Plasmid name: pCMV-mCherry-RAB7A(human)-Neo
Alias: NM_004637.6;CMT2B; PRO2706; RAB7
Gene lenght: 624bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-RAB7A-Neo plasmid Description

pCMV-mCherry-RAB7A-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-RAB7A-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tcaagcttcg aattccggtg gaggcggatc aggaggcggt 1381 ggatctatga cctctaggaa gaaagtgttg ctgaaggtta tcatcctggg agattctgga 1441 gtcgggaaga catcactcat gaaccagtat gtgaataaga aattcagcaa tcagtacaaa 1501 gccacaatag gagctgactt tctgaccaag gaggtgatgg tggatgacag gctagtcaca 1561 atgcagatat gggacacagc aggacaggaa cggttccagt ctctcggtgt ggccttctac 1621 agaggtgcag actgctgcgt tctggtattt gatgtgactg cccccaacac attcaaaacc 1681 ctagatagct ggagagatga gtttctcatc caggccagtc cccgagatcc tgaaaacttc 1741 ccatttgttg tgttgggaaa caagattgac ctcgaaaaca gacaagtggc cacaaagcgg 1801 gcacaggcct ggtgctacag caaaaacaac attccctact ttgagaccag tgccaaggag 1861 gccatcaacg tggagcaggc gttccagacg attgcacgga atgcacttaa gcaggaaacg 1921 gaggtggagc tgtacaacga atttcctgaa cctatcaaac tggacaagaa tgaccgggcc 1981 aaggcctcgg cagaaagctg cagttgctga ggatccaccg gatctagata actgatcata 2041 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 2101 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 2161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 2221 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt 2281 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 2341 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 2401 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 2461 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 2521 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 2581 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 2641 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 2701 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 2761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 2821 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg 2881 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2941 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3061 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3121 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3181 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc 3241 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 3301 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 3361 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 3421 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 3481 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 3541 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 3601 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 3661 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 3721 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat 3781 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 3841 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 3901 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 3961 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4021 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 4081 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4141 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4201 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga 4261 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt 4321 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 4381 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 4441 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca 4501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 4561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 4621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 4681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 4741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 4801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 4861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 4921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 4981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 5281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 5341 accgccatgc at

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.