pLV2-CMV-CALCR-FLAG-Puro plasm

pLV2-CMV-CALCR-FLAG-Puro plasmid

PVT96673

pLV2-CMV-CALCR-FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-CALCR(human)-FLAG-Puro
Alias: NM_001164737.3;CRT; CT-R; CTR; CTR1
Gene lenght: 1524bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-CALCR-FLAG-Puro plasmid Description

pLV2-CMV-CALCR-FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-CALCR-FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc 2821 ATGCAATTTT CTGGAGAGAA GATTAGTGGA CAAAGAGATC TTCAAAAATC AAAAATGAGG 2881 TTCACATTTA CAAGCCGGTG CTTGGCACTG TTTCTTCTTC TAAATCACCC AACCCCAATT 2941 CTTCCTGCCT TTTCAAATCA AACCTATCCA ACAATAGAGC CCAAGCCATT TCTTTACGTC 3001 GTAGGACGAA AGAAGATGAT GGATGCACAG TACAAATGCT ATGACCGAAT GCAGCAGTTA 3061 CCCGCATACC AAGGAGAAGG TCCATATTGC AATCGCACCT GGGATGGATG GCTGTGCTGG 3121 GATGACACAC CGGCTGGAGT ATTGTCCTAT CAGTTCTGCC CAGATTATTT TCCGGATTTT 3181 GATCCATCAG AAAAGGTTAC AAAATACTGT GATGAAAAAG GTGTTTGGTT TAAACATCCT 3241 GAAAACAATC GAACCTGGTC CAACTATACT ATGTGCAATG CTTTCACTCC TGAGAAACTG 3301 AAGAATGCAT ATGTTCTGTA CTATTTGGCT ATTGTGGGTC ATTCTTTGTC AATTTTCACC 3361 CTAGTGATTT CCCTGGGGAT TTTCGTGTTT TTCAGAAAAT TGACAACTAT TTTTCCTTTG 3421 AATTGGAAAT ATAGGAAGGC ATTGAGCCTT GGCTGCCAAA GGGTAACCCT GCACAAGAAC 3481 ATGTTTCTTA CTTACATTCT GAATTCTATG ATTATCATCA TCCACCTGGT TGAAGTAGTA 3541 CCCAATGGAG AGCTCGTGCG AAGGGACCCG GTGAGCTGCA AGATTTTGCA TTTTTTCCAC 3601 CAGTACATGA TGGCCTGCAA CTATTTCTGG ATGCTCTGTG AAGGGATCTA TCTTCATACA 3661 CTCATTGTCG TGGCTGTGTT TACTGAGAAG CAACGCTTGC GGTGGTATTA TCTCTTGGGC 3721 TGGGGGTTCC CGCTGGTGCC AACCACTATC CATGCTATTA CCAGGGCCGT GTACTTCAAT 3781 GACAACTGCT GGCTGAGTGT GGAAACCCAT TTGCTTTACA TAATCCATGG ACCTGTCATG 3841 GCGGCACTTG TGGTCAATTT CTTCTTTTTG CTCAACATTG TCCGGGTGCT TGTGACCAAA 3901 ATGAGGGAAA CCCATGAGGC GGAATCCCAC ATGTACCTGA AGGCTGTGAA GGCCACCATG 3961 ATCCTTGTGC CCCTGCTGGG AATCCAGTTT GTCGTCTTTC CCTGGAGACC TTCCAACAAG 4021 ATGCTTGGGA AGATATATGA TTACGTGATG CACTCTCTGA TTCATTTCCA GGGCTTCTTT 4081 GTTGCGACCA TCTACTGCTT CTGCAACAAT GAGGTCCAAA CCACCGTGAA GCGCCAATGG 4141 GCCCAATTCA AAATTCAGTG GAACCAGCGT TGGGGGAGGC GCCCCTCCAA CCGCTCTGCT 4201 CGCGCTGCAG CCGCTGCTGC GGAGGCTGGC GACATCCCAA TTTACATCTG CCATCAGGAG 4261 CTGAGGAATG AACCAGCCAA CAACCAAGGC GAGGAGAGTG CTGAGATCAT CCCTTTGAAT 4321 ATCATAGAGC AAGAGTCATC TGCTGACTAC AAGGACGACG ATGACAAGTG Aactagttct 4381 agagcggccg cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc 4441 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 4501 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 4561 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 4621 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 4681 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 4741 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 4801 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 4861 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc 4921 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc 4981 acaacccaca aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac 5041 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 5101 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 5161 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 5221 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 5281 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 5341 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 5401 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 5461 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 5521 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 5581 catgacccgc aagcccggtg cctagacgcg tctggaacaa tcaacctctg gattacaaaa 5641 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 5701 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 5761 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 5821 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 5881 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 5941 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 6001 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 6061 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 6121 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 6181 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa 6241 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga 6301 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat 6361 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg 6421 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg 6481 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt 6541 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg 6601 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga 6661 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca 6721 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga 6781 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat 6841 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg 6901 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 6961 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7021 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 7081 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac 7141 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 7201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 7261 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 7321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 7381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 8641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 8701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 9001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 9061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 9121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 9181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 9241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 9301 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt 9361 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 9421 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 9481 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 9541 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt 9601 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 9661 attgtatttg ttaaatatgt actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.