pLV3-U6-ADRA2A-shRNA3-CopGFP-P

pLV3-U6-ADRA2A-shRNA3-CopGFP-Puro plasmid

PVT96645

pLV3-U6-ADRA2A-shRNA3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-ADRA2A(human)-shRNA3-CopGFP-Puro
Alias: AGGCTTGGTGCGAGATCTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-ADRA2A-shRNA3-CopGFP-Puro plasmid Description

pLV3-U6-ADRA2A-shRNA3-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-ADRA2A-shRNA3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg AGGCTTGGTG CGAGATCTAc tcgagTAGAT 2221 CTCGCACCAA GCCTtttttt ggatccgcgg ccgcGaagga tctgcgatcg ctccggtgcc 2281 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 2341 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 2401 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 2461 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 2521 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 2581 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 2641 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 2701 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 2761 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 2821 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 2881 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 2941 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 3001 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 3061 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 3121 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 3181 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 3241 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 3301 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 3361 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 3421 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 3481 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 3541 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 3601 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 3661 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 3721 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 3781 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 3841 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 3901 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 3961 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4021 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4081 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 4141 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 4201 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 4261 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 4321 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 4381 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 4441 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 4501 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 4561 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 4621 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 4681 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 4741 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg 4801 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 4861 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 4921 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 4981 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 5041 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 5101 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 5161 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 5221 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 5281 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 5341 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 5401 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 5461 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 5521 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 5581 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 5641 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5701 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6421 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6961 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 7261 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 7321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 7381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 7441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 7621 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 7681 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 7741 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 7801 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 7861 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 7921 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 7981 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 8041 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 8101 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.