pCMV-DTX1-3×HA-Neo plasmid

pCMV-DTX1-3×HA-Neo plasmid

PVT96616

pCMV-DTX1-3×HA-Neo plasmid Information

Plasmid name: pCMV-DTX1(human)-3×HA-Neo
Alias: NM_004416.3;hDx-1; RNF140
Gene lenght: 1860bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-DTX1-3×HA-Neo plasmid Description

pCMV-DTX1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-DTX1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtc acggccaggc 721 cacggtgggc tgatgcctgt gaatggtctg ggcttcccac cgcagaacgt ggcccgggtg 781 gtggtgtggg agtggctgaa tgagcacagc cgctggcggc cctacacggc caccgtgtgc 841 caccacattg agaacgtgct gaaggaggac gctcgcggtt ccgtggtcct ggggcaggtg 901 gacgcccagc ttgtgcccta catcatcgac ctgcagtcca tgcaccagtt tcgccaggac 961 acaggcacca tgcggcccgt gcggcgcaac ttctacgacc cgtcgtcggc gccgggcaag 1021 ggcatcgtgt gggagtggga gaacgacggc ggcgcatgga cggcctacga tatggacatc 1081 tgcatcacca tccagaacgc ctacgagaag cagcacccgt ggctcgacct ctcatcgcta 1141 ggcttctgct acctcatcta cttcaacagc atgtcgcaga tgaaccgcca gacgcgccgg 1201 cgccgccgcc tgcgccgccg cctggacctc gcctacccgc tcaccgtggg ctccatccct 1261 aagtcgcagt cgtggcccgt gggcgccagc tcgggccagc cctgctcctg ccagcagtgc 1321 ctgctggtca acagcacgcg cgccgcctcc aacgccatcc tggcctcgca gcgccgcaag 1381 gcgccccccg cgcccccgct gccgccgccg ccgccacctg gagggcctcc aggcgcgctt 1441 gccgtgcgcc ccagcgccac cttcacaggc gccgcgctct gggcagcgcc cgccgccggc 1501 cccgccgagc ccgcgccgcc tcccggggcg cccccacgga gcccgggcgc ccccggcgga 1561 gcgcgcaccc cggggcagaa caacctcaac cggcccgggc cccagcgcac caccagcgtg 1621 agcgcgcgcg cctccatccc gccgggggtc cccgcactcc cggtgaagaa cttgaatggt 1681 actgggccgg tccatccggc cctggcaggg atgaccggga tactgctgtg cgcggccggg 1741 ctgcccgtgt gcctgacgcg ggcccccaag cccatcctgc acccgccgcc cgtgagcaag 1801 agcgacgtga agcccgtgcc tggcgtgccc ggggtgtgcc gcaagaccaa gaagaagcac 1861 cttaaaaaga gtaagaatcc cgaggatgtg gttcgaagat acatgcagaa ggtgaaaaac 1921 ccacctgatg aggactgcac catctgcatg gagcgactgg tcacagcatc aggctacgag 1981 ggcgtgcttc ggcacaaggg cgtgcggcct gagctcgtgg gccgcctggg ccgctgtggc 2041 cacatgtacc acctgctgtg cctcgtggcc atgtactcca atggcaacaa ggatggcagc 2101 ctgcagtgcc ccacctgcaa ggccatctac ggggagaaga cgggtacgca gccgcctggg 2161 aagatggagt tccacctcat cccccactcg ctgcccggct tccctgatac ccagaccatc 2221 cgcatcgtct atgacatccc cacaggcatc cagggccctg agcaccccaa ccccgggaag 2281 aagttcaccg caagaggatt ccctcgccac tgctatctac ccaacaacga gaaaggccgg 2341 aaggtgctgc ggctgctcat cacggcctgg gagagaagac tcatcttcac tatcggcacg 2401 tccaacacca cgggcgagtc ggacaccgtg gtgtggaacg agatccacca caagaccgag 2461 tttggatcca acctcacggg ccacggctac ccggacgcta gctacctaga caacgtgctg 2521 gctgagctca cagcccaggg cgtatccgag gctgcagcca aggctCTCGA GTACCCATAC 2581 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 2641 GATGTCCCAG ACTACGCATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA 2701 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 2761 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 2821 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 2881 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 2941 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 3001 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 3061 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 3121 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 3181 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 3241 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG 3301 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC 3361 GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 3421 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 3481 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 3541 CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 3601 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 3661 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 3721 CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 3781 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 3841 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 3901 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA 3961 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT 4021 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 4081 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 4141 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 4201 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 4261 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 4321 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 4381 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 4441 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 4501 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 4561 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 4621 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 4681 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 4741 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 4801 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 4861 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 4921 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 4981 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 5041 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 5101 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 5161 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 5221 GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 5281 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 5341 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 5401 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 5461 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 5521 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 5581 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 5641 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 5701 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 5761 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 5821 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 5881 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 5941 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 6001 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 6061 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 6121 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC 6181 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 6241 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 6301 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 6361 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 6421 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 6481 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 6541 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 6601 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 6661 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 6721 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 6781 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 6841 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 6901 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 6961 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 7021 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 7081 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 7141 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 7201 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 7261 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 7321 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 7381 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 7441 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 7501 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 7561 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 7621 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 7681 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 7741 CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA 7801 TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC 7861 AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.