pCMV-TTC17-3×HA-Neo plasmid

pCMV-TTC17-3×HA-Neo plasmid

PVT96504

pCMV-TTC17-3×HA-Neo plasmid Information

Plasmid name: pCMV-TTC17(human)-3×HA-Neo
Alias: NM_018259.6
Gene lenght: 3423bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TTC17-3×HA-Neo plasmid Description

pCMV-TTC17-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TTC17-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCg ccaccatggc ggcggcagta 721 ggggttcgtg gccggtacga gctgccgcct tgctccggcc caggctggct cctcagcctt 781 tccgccttgc tgagtgtggc ggcacgaggg gccttcgcca ccacgcactg ggtcgtcacg 841 gaggacggga aaatccagca gcaggtggat tcaccaatga acttgaagca tcctcatgac 901 ctagtcatat taatgagaca agaagcaaca gttaactacc tcaaagaatt agagaaacaa 961 ttagttgctc aaaaaattca catagaagag aatgaggaca gagacacagg actggaacag 1021 agacataata aagaagaccc agactgcatc aaagccaagg tgcccttagg ggacctggat 1081 ctatatgatg gcacatacat aactttggag agcaaagaca tcagtcctga agattatata 1141 gacacagaat ctcctgtccc tccagaccca gagcaacctg attgtactaa aattctagaa 1201 cttccatata gtatacatgc ttttcagcac ttgagaggtg tacaggagag agttaatctt 1261 tctgcacctc tgctacctaa agaagaccca atcttcacat atttatctaa acggttagga 1321 aggagtatag atgacatagg tcacctcatt catgaaggcc tacagaagaa cacttcctcg 1381 tgggtactgt ataacatggc ttcattttac tggagaatta agaatgagcc atatcaggta 1441 gtagaatgtg ccatgcgagc acttcacttc tcttccaggc acaataaaga cattgccctg 1501 gtcaacctgg caaacgttct acacagagca cacttctctg ctgatgctgc tgtcgtggtc 1561 catgcagctc tggatgacag tgacttcttc accagctatt acactttggg gaatatatat 1621 gcaatgcttg gggaatataa ccactcagtg ctctgttatg accacgcttt gcaggccaga 1681 cctgggtttg agcaagctat aaagaggaag catgctgtcc tatgtcagca aaaactggag 1741 cagaaattgg aggctcagca tagatctctc cagcgaacac tgaatgagtt aaaagagtat 1801 caaaagcagc atgaccacta cctgagacag caggaaatcc tagaaaaaca taaactgatt 1861 caggaggagc aaatcttaag aaatatcatt catgagactc agatggcaaa agaggcacaa 1921 ttaggaaatc atcagatatg ccgactggtc aaccagcagc atagtttaca ttgccagtgg 1981 gaccagcctg tacgctatca tcgtggagat atctttgaaa atgtggacta tgttcagttt 2041 ggtgaggatt catcaacctc cagtatgatg tctgtgaact ttgatgttca atcaaatcag 2101 agtgatatca atgattcggt caagtcttct cccgtagccc attctattct ctggatttgg 2161 ggcagggact ctgatgcata tagggacaaa cagcatattc tatggcctaa aagagcagat 2221 tgtacagaaa gctaccctag agtccctgtt ggtggggaat tgccaacgta ttttctgcct 2281 ccggaaaaca aaggactcag gatccacgaa ctcagcagtg atgattattc tacagaagaa 2341 gaggcccaaa cccctgactg ttccataact gacttcagaa aaagccacac tctgtcctac 2401 ttagtcaaag aattagaggt tcgcatggat ctgaaagcca aaatgccaga tgaccatgca 2461 cgaaaaattt tgctttcccg tattaataac tatactatcc cagaagaaga aattgggtct 2521 ttcttatttc atgctattaa taagccaaat gctcctatct ggctcatact caatgaagct 2581 ggactatact ggagagcagt aggaaatagc acttttgcta ttgcctgtct tcagagggct 2641 ttgaatttag ctccacttca ataccaagat gttcctcttg tcaacttggc caaccttttg 2701 attcattacg gccttcatct tgatgccact aagctgctac ttcaagcttt ggccatcaat 2761 agctctgagc ctctgacctt tttgagcctg ggaaatgctt accttgctct gaagaatatc 2821 agtggggcac ttgaggcctt tagacaggcc ttgaaattaa ccaccaaatg tccagagtgt 2881 gaaaacagcc tgaagttgat ccgctgtatg cagttttatc cttttctgta caacatcact 2941 tcttctgttt gcagtggtac ggtggttgag gagagcaatg gttctgatga gatggagaat 3001 tcagatgaaa ccaaaatgtc agaagaaata ctggctttgg tggatgaatt tcaacaggca 3061 tggcctttgg aaggctttgg gggtgcacta gagatgaaag ggcggcgtct agacttacaa 3121 ggaatacggg tgctgaagaa aggtccccag gatggagtgg ccagaagctc ttgctatgga 3181 gactgcagaa gtgaagatga tgaagcaaca gaatggatta cattccaggt caaacgtgta 3241 aagaaaccca aaggagatca taagaaaact cctgggaaaa aagtagaaac aggtcagata 3301 gaaaatggac atcgttacca agcaaaccta gagatcactg gccccaaggt ggcatctcct 3361 gggccacaag gaaaaaaacg tgactaccag cgtctgggat ggcccagccc ggacgaatgc 3421 ctcaaactcc gctgggtaga gctgactgcc atcgtgagta cctggcttgc agtttcttca 3481 aaaaacattg acatcacaga acacatagat tttgccaccc ctatacagca gccagcaatg 3541 gagcctcttt gcaatggcaa tctccccacg agtatgcata ccctggacca cttgcatggg 3601 gtttccaacc gagccagcct gcactacaca ggggagagtc agttaacaga ggtattacaa 3661 aatctcggca aagaccaata tccacaacag tcgcttgaac agattggcac ccgaattgcc 3721 aaagttttgg aaaagaacca gacgtcctgg gtcctctcca gcatggcagc cctctactgg 3781 agggtgaaag gccaaggaaa gaaggcaatc gactgcctcc gccaggctct gcactatgcg 3841 ccacaccaga tgaaggatgt gcccctgatt agcctggcca acatcttgca caatgccaag 3901 ctctggaatg acgccgtcat agtagccacc atggcagtag agatcgcacc acactttgct 3961 gtgaaccact tcactctggg caatgtctac gtggcaatgg aagaatttga aaaagcactg 4021 gtgtggtatg aatccacatt gaagcttcag cccgagtttg tcccagccaa gaaccgaatc 4081 cagaccatcc agtgtcactt aatgctgaag aagggacggc gctctcctCT CGAGTACCCA 4141 TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT 4201 TATGATGTCC CAGACTACGC ATAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT 4261 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 4321 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 4381 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 4441 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 4501 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 4561 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 4621 CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT 4681 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 4741 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 4801 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC 4861 ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA 4921 AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT 4981 CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC 5041 CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG 5101 GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA 5161 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 5221 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 5281 TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 5341 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 5401 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 5461 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG 5521 GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT 5581 TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 5641 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC 5701 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 5761 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG 5821 ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA 5881 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT 5941 ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA 6001 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 6061 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC 6121 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC 6181 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 6241 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC 6301 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 6361 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG 6421 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT 6481 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT 6541 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG 6601 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 6661 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG 6721 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 6781 AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 6841 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 6901 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 6961 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 7021 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 7081 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 7141 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 7201 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 7261 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 7321 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 7381 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 7441 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 7501 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 7561 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 7621 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 7681 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT 7741 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 7801 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 7861 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 7921 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 7981 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 8041 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 8101 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 8161 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 8221 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 8281 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 8341 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 8401 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 8461 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 8521 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 8581 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 8641 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 8701 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 8761 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 8821 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 8881 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 8941 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 9001 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 9061 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 9121 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 9181 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 9241 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC 9301 TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA 9361 GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC 9421 TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.