pMSCV-Nr1d2-3×FLAG-UBC-EGFP pl

pMSCV-Nr1d2-3×FLAG-UBC-EGFP plasmid

PVT89170

pMSCV-Nr1d2-3×FLAG-UBC-EGFP plasmid Information

Plasmid name: pMSCV-Nr1d2(mouse)-3×FLAG-UBC-EGFP
Alias: NM_011584.5;Rev-erb; RVR
Gene lenght: 1728bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pBABE-5
Reverse primer:
Copy number: High
Induction:

pMSCV-Nr1d2-3×FLAG-UBC-EGFP plasmid Description

pMSCV-Nr1d2-3×FLAG-UBC-EGFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMSCV-Nr1d2-3×FLAG-UBC-EGFP plasmid Sequence

1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT 61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA 121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT 241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC 301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC 361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA 421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA 481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA 541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG 601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT 661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA 721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA 781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG 841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA 901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG 961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GGCCACCatg gagctgaacg 1441 caggaggtgt gattgcctac atcagttcct ctagctctgc ctccagccct gcctcttgtc 1501 acagtgaggg ttctgagaat agcttccagt cctcgtcctc gtctgttcca tcttctccaa 1561 atagctctaa ctgtgatgcc aacggcaatc ccaagaacgc tgatatctct agcatcgatg 1621 gtgttctgaa gagtgaccgc acagattgtc ctgtgaaaac aggcaaaacc agtgctcctg 1681 gcatgactaa gagtcacagt ggaatgacaa aatttagtgg catggttcta ctgtgtaaag 1741 tctgtgggga tgtggcatca ggattccact atggagttca tgcttgtgaa ggctgtaagg 1801 gtttctttcg gaggagcatt cagcaaaaca tccagtataa gaagtgcctg aagaatgaga 1861 actgttccat catgaggatg aacaggaacc ggtgccagca gtgccgcttt aagaagtgtc 1921 tgtctgtggg gatgtcacga gatgctgttc gatttggccg aattcctaag cgtgaaaaac 1981 agagaatgct aattgaaatg caaagtgcaa tgaagaccat gatgaacacc cagttcagtg 2041 gccacctgca gaatgacacc ttagcagaac agcatgatca gtcagcacta ccagctcagg 2101 aacagctgcg gcccaagtcc cagctggagc aagaaaacat caaaaacact ccttctgatt 2161 ttgcaaagga ggaagtgatt ggtatggtga ccagagccca caaggatacc tttctgtata 2221 atcaggaaca tcgagaaaac tcatctgaga gcatgccacc tcagagagga gaacggattc 2281 ccaggaacat ggagcaatat aatttaaatc aagaccatcg tggcagtggg attcacaacc 2341 acttcccctg tagtgagagg cagcaacatc tcagtggaca gtacaaaggg aggaacataa 2401 tgcattaccc aaacggccat gccgtttgta ttgcaaatgg acactgtatg aacttctcca 2461 gtgcttatac tcaaagagtc tgtgatagaa ttccagtagg tggatgttct cagactgaga 2521 acagaaatag ttacctgtgc aacactggag ggaggatgca tctggtgtgt cctatgagca 2581 agtctccata tgtggaccct cagaagtctg gacatgaaat ctgggaagaa ttttcaatga 2641 gttttacccc agcagtaaaa gaggtggtgg aatttgcaaa gaggattcct ggcttccgag 2701 atctgtctca gcatgatcag gtcaatctgt taaaagctgg gacttttgag gttttaatgg 2761 tacgatttgc ttcattattt gatgcaaagg aacggactgt cacctttcta agtggtaaga 2821 agtacagtgt ggatgacctg cactcaatgg gagcagggga tctgctcagc tctatgtttg 2881 agttcagtga gaagctgaat gccctccagc tcagtgatga ggaaatgagc ttgttcacag 2941 cagttgttct ggtatctgca gatcgatctg gaattgaaaa tgtcaactca gtggaggctt 3001 tgcaggaaac actcatccgt gcactaagga ccttaataat gaaaaaccat ccaaatgagg 3061 cctccatttt tacaaaatta cttctaaagt tgccagatct tcgatcttta aacaacatgc 3121 actctgagga actcttggcc tttaaagttc atcctGGATC CGACTACAAA GACCATGACG 3181 GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG GAATTCgtac 3241 gacgcgtatg catggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc 3301 acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg 3361 acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag 3421 aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc 3481 ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc 3541 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag 3601 ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc 3661 gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt 3721 gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg 3781 gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg 3841 aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag 3901 gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc 3961 atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg 4021 cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg 4081 ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct 4141 gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta 4201 ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca 4261 gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac 4321 tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta 4381 atcatttggg tcaatatgta attttcagtg ttagACTAGT AAATTGTCCG CTAAATTCTG 4441 GCCGTTTTTG GCTTTTTTGT TAGACGAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC 4501 CGGGTACCGG TCGCCACCat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 4561 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 4621 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 4681 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 4741 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 4801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 4861 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 4921 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 4981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 5041 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 5101 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 5161 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 5221 gacgagctgt acaagtaaAT CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG 5281 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA 5341 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA 5401 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC 5461 CAAGAACAGA TGGTCCCCAG ATGCGGTCCC GCCCTCAGCA GTTTCTAGAG AACCATCAGA 5521 TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC 5581 AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC 5641 AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TAGACTGCGT CGCCCGGGTA CCCGTGTATC 5701 CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC 5761 CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATG GGTAACAGTT TCTTGAAGTT 5821 GGAGAACAAC ATTCTGAGGG TAGGAGTCGA ATATTAAGTA ATCCTGACTC AATTAGCCAC 5881 TGTTTTGAAT CCACATACTC CAATACTCCT GAAATAGTTC ATTATGGACA GCGCAGAAGA 5941 GCTGGGGAGA ATTAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 6001 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 6061 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 6121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 6181 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 6241 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 6301 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 6361 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 6421 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 6481 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 6541 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 6601 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 6661 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 6721 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 6781 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 6841 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 6901 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 6961 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 7021 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 7081 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 7141 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 7201 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 7261 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 7321 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 7381 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 7441 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 7501 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 7561 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 7621 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 7681 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 7741 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 7801 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 7861 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 7921 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 7981 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 8041 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 8101 TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA 8161 AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 8221 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG 8281 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 8341 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG 8401 CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA 8461 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC 8521 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC 8581 GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC 8641 ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA 8701 TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC 8761 ACGATGCGTC CGGCGTAGAG GCGATTAGTC CAATTTGTTA AAGACAGGAT ATCAGTGGTC 8821 CAGGCTCTAG TTTTGACTCA ACAATATCAC CAGCTGAAGC CTATAGAGTA CGAGCCATAG 8881 ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.