pESC-URA-SAM2-FLAG-ADK1(yeast)

pESC-URA-SAM2-FLAG-ADK1(yeast)-Myc plasmid

PVT96506

pESC-URA-SAM2-FLAG-ADK1(yeast)-Myc plasmid Information

Plasmid name: pESC-URA-SAM2(yeast)-FLAG-ADK1(yeast)-Myc
Alias: NM_001180810.3; NM_001180534.1
Gene lenght: 1152，666bp
Host: Yeast
Use(s): Protein Expression
Fragment type: ORF
Species: Yeast
Prokaryotic resistance: Amp
Screening marker: URA3
Fluorescent labeling:
Promoter: GAL1,10
Replicon: 2U
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pESC-URA-SAM2-FLAG-ADK1(yeast)-Myc plasmid Description

pESC-URA-SAM2-FLAG-ADK1(yeast)-Myc This is a plasmid suitable for protein expression in yeast.

pESC-URA-SAM2-FLAG-ADK1(yeast)-Myc plasmid Sequence

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 1861 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga 1921 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 1981 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 2041 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca 2101 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca 2161 aacctctggc gaagaattgt taattaagag ctcagatctt atcgtcgtca tccttgtaat 2221 ccatcgatac tagtgcggcc gcAAATTCCA ATTTCTTTGG TTTTTCCCAT GAGTACTCTT 2281 GATTAGTGAA GTGACCATAA GAAGCGGTTG GTAAGTAAAT TGGTCTAGCC AAATCTAATT 2341 CCTTTACTAA CACACCTGGT CTCAAGTCGA AGTTCTTCTT AATAATTTCA ATGATTTCGT 2401 CATCTGATTT TGTAGCTGTA CCATAGGTGT CCACATGTAA AGACAATGGT TCAGCAATAC 2461 CAATAGCATA TGAAAATTGG ACTTGGACTC TCTTACACAA ACCAGCGGCA ACTAGAGACT 2521 TGGCAACCCA TCTAGCAGCG TAAGCAGCGG AACGATCGAC CTTGGAATAG TCCTTACCGG 2581 AGAAGGCACC ACCACCGACG GATGAGGCAC CACCGTAAGC GTCGACAATA ATCTTTCTAC 2641 CGGTCAAACC AGCGTCACCT TGAGGACCAC CGATGACGAA TCTACCGGAT GGTTGGATGA 2701 AATATTTGGT ATTTTCGTCT AACATATCCT TTGGTATGAC CTTTTCAACA ATATCTTTTT 2761 GAAGTTGAGT TCTCAAGTCA GCGGTGGAAA TTTCATCAGC ATGTTGAGCA GAAATAACAA 2821 CGGTATCTAT CCTCTTTGGA ACCCATCTAC CATTGTCGTC TTCGTATTCG ACAGTGACTT 2881 GAGTCTTTGT GTCTGGTCTC AACCATGGGA GAGAACCATC TCTTCTAGCA TCTGCCATAG 2941 CCATGTTCAA TTTGTGAGCC AAAAGAATGG TCAATGGTAA CCCTTCTGGA GTTTCGTCTG 3001 TAGCGTAACC AAACATTATA CCTTGGTCAC CAGCACCTAA GTCTTCTAAG CTCTTTTCAT 3061 AGTGCAGACC TTGAGCGATA TCTGGAGATT GTTGTTCGAT AGCTACTAAA ACATTACATG 3121 TCTTGTAGTC GAAACCCTTG GCAGAATCGT CATAACCAAT CTTCTTGATG GTATCTCTTA 3181 CTATTTGTTG GTAGTCAAGT CTAGCTTTGG TGGTAATTTC ACCGAAAACC ATAATCATAC 3241 CAGTTTTGGC AGCTGTTTCA CAGGCAACCT TGGAGAATGG ATCTTGTTCT AAACAAGCGT 3301 CCAAAATAGC ATCAGAAACT TGGTCACAAA TCTTGTCTGG GTGACCTTCA CCGACGGATT 3361 CAGAGGTAAA TAAGAAAGTT TTGCTCTTGG ACATggtggc gaattcgaat tttcaaaaat 3421 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc 3481 accatataca tatccatata catatccata tctaatctta cttatatgtt gtggaaatgt 3541 aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct 3601 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc 3661 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga 3721 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt 3781 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat 3841 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc 3901 agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact gcataaccac 3961 tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat gtaataaaag 4021 tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaaccccgg 4081 atccgtaata cgactcacta tagggcccgg gcgtcgacgc caccATGTCT AGCTCAGAAT 4141 CCATTAGAAT GGTCCTAATT GGCCCACCTG GTGCCGGTAA AGGTACTCAA GCTCCAAATT 4201 TGCAAGAGCG TTTCCATGCC GCTCACTTGG CCACTGGTGA CATGTTGAGA TCTCAAATCG 4261 CAAAGGGCAC TCAATTAGGT TTGGAAGCAA AGAAAATTAT GGACCAAGGT GGTTTAGTCT 4321 CTGATGACAT TATGGTTAAC ATGATCAAGG ATGAATTGAC CAACAATCCA GCTTGTAAGA 4381 ATGGGTTCAT CTTGGACGGT TTCCCAAGAA CCATTCCTCA GGCTGAAAAA TTGGACCAAA 4441 TGTTGAAAGA ACAAGGAACT CCTTTGGAAA AAGCCATCGA ATTGAAGGTT GATGATGAAT 4501 TGTTGGTTGC CAGAATTACC GGTAGATTAA TTCACCCAGC CTCTGGCAGA TCCTACCACA 4561 AGATCTTTAA CCCACCAAAG GAAGACATGA AGGATGACGT CACCGGTGAA GCTTTAGTTC 4621 AAAGATCTGA TGACAATGCA GACGCCTTGA AGAAGAGATT AGCTGCTTAC CATGCTCAAA 4681 CCGAACCAAT TGTTGACTTT TACAAAAAGA CCGGTATCTG GGCTGGTGTT GATGCTTCCC 4741 AACCTCCTGC TACTGTTTGG GCTGACATCT TGAACAAGCT AGGTAAGGAT gaacagaagt 4801 tgatttccga agaagacctc gagtaagctt ggtaccgcgg ctagctaaga tccgctctaa 4861 ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta 4921 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg 4981 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga 5041 tccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 5101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 5161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 5221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 5281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 5341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 5401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 5461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 5521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 5581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 5641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 5701 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 5761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 5821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 5881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 5941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 6001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 6061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 6121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 6181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 6241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 6301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 6361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 6421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 6481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 6541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 6601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 6661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 6721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 6781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 6841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 6901 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 6961 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 7021 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga 7081 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 7141 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg 7201 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 7261 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg 7321 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg 7381 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga 7441 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt 7501 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata 7561 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc 7621 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag 7681 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt 7741 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat 7801 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat 7861 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag 7921 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt 7981 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac 8041 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 8101 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 8161 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 8221 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 8281 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 8341 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg 8401 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.