pCMV-GLI3-3×FLAG-Neo plasmid

pCMV-GLI3-3×FLAG-Neo plasmid

PVT96496

pCMV-GLI3-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-GLI3(human)-3×FLAG-Neo
Alias: NM_000168.6;ACLS; GCPS; GLI3-190; GLI3FL; PAP-A; PAPA;PAPA1; PAPB; PHS; PPDIV;
Gene lenght: 4740bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GLI3-3×FLAG-Neo plasmid Description

pCMV-GLI3-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GLI3-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga ggcccagtcc cacagctcca 781 cgaccactga aaagaaaaaa gttgagaatt ccatagtgaa gtgctccact cgaacagatg 841 tgagcgagaa agccgttgcc tccagcacca cttctaatga ggatgaaagt cctggacaga 901 cttatcacag agagagaaga aacgcaatca ctatgcagcc acagaatgtc caggggctca 961 gcaaagtcag tgaggaacct tcaacatcga gtgacgagag ggcctcattg atcaagaaag 1021 agatccatgg gtccctgcca cacgtggcgg agccctctgt gccgtaccgc gggacggtgt 1081 ttgccatgga ccccaggaat ggttacatgg agccccacta ccaccctcct catcttttcc 1141 ctgccttcca tcctcctgta ccaattgatg ccagacatca tgagggccgt taccattacg 1201 atccatctcc gattcctcca ttgcatatga cttccgcctt atctagtagc cctacgtatc 1261 cggacctgcc cttcattagg atctccccac accggaaccc cactgctgct tccgagtctc 1321 ccttcagccc tccacatccc tacattaatc cctacatgga ctatatccgc tccttgcaca 1381 gcagcccatc gctctccatg atctcagcaa cccgtgggct gagccctaca gatgcgcccc 1441 atgcaggagt cagcccagca gaatactatc atcagatggc cctgctaact ggccagcgca 1501 gcccctatgc agacattatt ccctcagctg ccaccgccgg cacgggggcc atccacatgg 1561 aatatcttca tgctatggat agcaccagat tctccagccc caggctgtca gccaggccga 1621 gccgaaaacg tacactgtcc atatcaccac tctccgatca tagctttgac cttcagacca 1681 tgataaggac gtctcccaac tccttggtca cgattctcaa taattcccgt agcagctctt 1741 cagcaagtgg ctcctatggt cacttatctg caagtgcaat cagccctgcc ttgagcttca 1801 cctactcttc cgcgcccgtc tctctccaca tgcatcagca gatcctaagc cgacaacaga 1861 gcttaggttc agcctttgga cacagccctc cactcatcca ccctgcccca acttttccaa 1921 cacagaggcc tattccaggg atccctacgg ttctgaaccc cgtccaggtc agctccggcc 1981 cttctgagtc ctcacagaac aagcccacga gtgagtctgc agtgagcagc actggtgacc 2041 cgatgcacaa caagaggtcc aagatcaaac ccgatgaaga cctccccagc ccaggggctc 2101 gggggcagca ggaacagccc gaaggaacaa cccttgtcaa ggaggaaggg gacaaagatg 2161 aaagcaaaca ggagcctgaa gtcatctatg agacaaactg ccactgggaa ggctgcgcga 2221 gggagttcga cacccaagag cagcttgtgc accatataaa taacgaccat attcatggag 2281 agaagaagga gttcgtgtgc aggtggctgg actgctcaag agagcagaaa cccttcaaag 2341 cccagtatat gttggtagtg catatgagaa gacacacggg cgagaagcct cacaaatgca 2401 cttttgaagg ttgcacaaag gcctactcga gactagaaaa cttgaaaaca cacttgagat 2461 ctcacactgg agagaaacca tacgtctgtg agcacgaagg ttgcaacaag gctttctcaa 2521 atgcctctga tcgcgccaaa caccaaaaca gaacgcattc caatgagaaa ccatatgtgt 2581 gcaaaatccc aggctgcact aagcgttaca cagacccaag ctccctccgg aaacatgtga 2641 agacagtgca tggcccagag gctcatgtca ccaagaagca gcgaggggac atccatcctc 2701 ggccgccacc cccgagagat tccggcagcc attcacagtc caggtcgcct ggccgaccga 2761 ctcagggagc ccttggtgag cagcaggacc tcagcaacac tacctcaaag cgggaagaat 2821 gcctccaggt gaaaaccgtc aaggcagaga agccaatgac atctcagcca agccctggtg 2881 gtcagtcttc atgcagcagc caacagtccc ccatcagcaa ctattccaac agtgggctcg 2941 agcttcctct gaccgatgga ggtagtatag gagacctcag tgccatcgat gaaaccccaa 3001 tcatggactc aaccatttcc actgcaacca cagcccttgc tttgcaagcc aggagaaacc 3061 cggcagggac caaatggatg gagcacgtaa aactagaaag gctaaaacaa gtgaatggaa 3121 tgtttccgcg actgaacccc attctacccc ctaaagcccc tgcggtctct cctctcatag 3181 gaaatggcac acagtccaac aacacctgca gcttgggtgg gcccatgacg cttctcccgg 3241 gcagaagcga cctctctggg gtggacgtca ctatgctgaa catgctcaac agaagggaca 3301 gcagcgccag caccatcagc tcggcctacc tgagcagccg ccgctcctca gggatctcgc 3361 cctgcttctc cagccgccgc tccagcgagg cgtcacaggc cgagggccgg ccgcagaacg 3421 tgagcgtggc cgactcctac gaccccatct ccaccgacgc ctcgcgccgc tccagcgaag 3481 ccagccagag cgacggcctg cccagcctgc tcagcctcac gcccgcccag cagtaccgcc 3541 tcaaggccaa gtacgcggct gccacaggag ggccgccgcc gacgcccctg cccaacatgg 3601 agaggatgag cctgaagacg cgcctggcgc tgctcgggga tgccctcgag cctggcgtgg 3661 ccctgcctcc agttcatgcc ccgaggaggt gcagcgacgg gggagcccac ggctacgggc 3721 ggcgccacct gcagccgcac gatgcgccgg gccacggcgt gaggagggcc agcgacccgg 3781 tgcggacagg ctccgagggc ctggccctgc ctcgtgtgcc gcgcttcagc agcctcagca 3841 gctgcaaccc cccggcgatg gccacgtccg cggagaagcg cagtctcgtg cttcagaatt 3901 acacgcggcc cgagggcggc cagtcccgaa acttccactc gtccccctgt cctcccagca 3961 tcaccgagaa cgtcaccctg gagtccctga ccatggacgc tgatgccaac ctgaacgatg 4021 aggatttcct gccggacgac gtggtgcagt atttaaattc ccagaaccaa gcagggtacg 4081 agcagcactt ccccagcgcc ctcccggacg acagcaaagt gccccacggg cccggtgact 4141 ttgacgcgcc cgggctgcca gacagccacg ctggccagca gttccatgcc ctcgagcagc 4201 cctgccccga gggcagcaaa accgacctgc ccattcagtg gaacgaagtc agctccggaa 4261 gcgccgacct gtcctcctcc aagctcaagt gtgggccgcg gcccgctgtg ccgcagactc 4321 gcgcctttgg gttctgcaac ggcatggtcg tccacccgca gaaccccttg aggagcgggc 4381 ctgctggggg ctatcagacc ctcggggaga acagcaaccc ctacggtggc ccagagcact 4441 tgatgctcca caacagcccc ggaagtggca ccagtggaaa cgccttccat gaacagccct 4501 gtaaggcccc gcagtatggg aactgtctca acaggcagcc agtggcccct ggtgcactcg 4561 acggtgcctg tggtgccggg attcaagcct caaagctgaa gagcaccccc atgcaaggga 4621 gcgggggcca gctgaatttc ggcctgccgg tagcgccaaa tgagtcagct ggcagcatgg 4681 tgaatggcat gcagaaccag gacccagtgg gacaggggta cctggctcac cagctcctcg 4741 gcgacagcat gcagcacccg ggggcaggcc gccccggtca gcagatgctt gggcagatta 4801 gtgctacctc acacatcaac atctaccaag ggccagagag ctgcctgcca ggggctcacg 4861 gcatgggcag ccagccgtca agcttggcag ttgtcagggg ctaccagcca tgtgccagct 4921 ttgggggcag caggcgccag gctatgccga gggacagcct tgctctgcag tcaggacagc 4981 tcagtgacac aagtcagacc tgcagggtga atggtatcaa gatggagatg aaagggcagc 5041 cccatccgct gtgctctaat ctgcagaatt actctggtca gttctatgac caaaccgtgg 5101 gcttcagtca gcaagacacg aaagctggtt cattctctat ttcagacgcc agctgcctgc 5161 tacaggggac cagcgccaaa aactctgagt tactttcccc aggtgctaat caggtgacaa 5221 gcacagtgga cagcctcgac agccatgacc tggaaggggt acagattgac ttcgatgcca 5281 tcatagacga tggggaccac tccagcctga tgtcgggggc cctgagccca agtatcattc 5341 agaacctttc ccatagctcc tcccgcctca ccacgcctcg ggcgtccctc ccattcccag 5401 cgctgtccat gagcaccacc aacatggcta tcggggacat gagttctttg ctgacctccc 5461 tagcggaaga aagcaaattc cttgcagtta tgcaaGGATC CGACTACAAA GACCATGACG 5521 GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG 5581 ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 5641 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 5701 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 5761 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 5821 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 5881 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 5941 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC 6001 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 6061 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 6121 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 6181 TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT 6241 ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG 6301 CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA 6361 ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG 6421 GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC 6481 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 6541 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 6601 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT 6661 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 6721 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 6781 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 6841 TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 6901 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 6961 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 7021 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 7081 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 7141 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 7201 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 7261 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 7321 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 7381 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 7441 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 7501 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 7561 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 7621 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 7681 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 7741 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 7801 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 7861 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 7921 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 7981 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 8041 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 8101 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA 8161 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 8221 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 8281 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 8341 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 8401 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 8461 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 8521 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 8581 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 8641 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 8701 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 8761 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 8821 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 8881 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 8941 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 9001 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 9061 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 9121 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 9181 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 9241 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 9301 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 9361 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 9421 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 9481 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 9541 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 9601 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 9661 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 9721 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 9781 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 9841 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 9901 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 9961 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 10021 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 10081 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 10141 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 10201 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 10261 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 10321 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 10381 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 10441 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 10501 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 10561 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 10621 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC 10681 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT 10741 GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG 10801 AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.