pLV3-CMV-NSDHL-3×FLAG-CopGFP-P

pLV3-CMV-NSDHL-3×FLAG-CopGFP-Puro plasmid

PVT96493

pLV3-CMV-NSDHL-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NSDHL(human)-3×FLAG-CopGFP-Puro
Alias: NM_015922.3;H105E3; SDR31E1; XAP104
Gene lenght: 1119bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NSDHL-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NSDHL-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NSDHL-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aaccagcagt tagcgagcca 2341 atgagagacc aagtcgcacg gactcatttg acagaggaca ctcccaaagt gaatgctgac 2401 atagaaaagg ttaaccagaa tcaggccaag agatgcacag tgatcggtgg ctctggattc 2461 ctggggcagc acatggtgga gcagttgctg gcaagaggat atgctgtcaa tgtatttgat 2521 atccagcaag ggtttgataa tccccaggtg cggttctttc tgggtgacct ctgcagccga 2581 caggatctgt acccagctct gaaaggtgta aacacagttt tccactgtgc gtcaccccca 2641 ccatccagta acaacaagga gctcttttat agagtgaatt acattggcac caagaatgtc 2701 attgaaactt gcaaagaggc tggggttcag aaactcattt taaccagcag tgccagtgtc 2761 atctttgagg gcgtcgatat caagaatgga actgaagacc ttccctatgc catgaaaccc 2821 attgactact acacagagac taagatctta caggagaggg cagttctggg cgccaacgat 2881 cctgagaaga atttcttaac cacagccatc cgccctcatg gcattttcgg cccaagggac 2941 ccgcagttgg tacccatcct catcgaggca gccaggaacg gcaagatgaa gttcgtgatt 3001 ggaaatggga agaacttggt ggacttcacc tttgtggaga acgtggtcca tggacacatc 3061 ctggcggcag agcagctctc ccgagactcg acactgggtg ggaaggcatt tcacatcacc 3121 aatgatgagc ccatcccttt ctggacattc ctgtctcgca tcctgacagg cctcaattat 3181 gaggccccca agtaccacat cccctactgg gtggcctact acctggccct cctgctatcc 3241 ctgctggtga tggtgatcag tcctgtcatc cagctgcagc ccaccttcac acccatgcgg 3301 gtcgcactgg ctggcacatt ccactactac agctgcgaga gagccaaaaa ggccatgggc 3361 taccagccac tagtgaccat ggatgatgct atggagagga ccgtgcagag ctttcgccac 3421 ctgcggaggg tcaagggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3481 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 3541 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3601 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3661 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3721 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3781 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3841 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3901 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3961 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4021 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4081 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4141 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4201 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4261 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4321 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4381 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4441 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4501 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4561 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4621 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4681 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4741 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4801 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4861 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4921 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4981 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5041 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5101 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5161 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5221 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5281 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5341 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5401 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5461 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5521 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5581 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5641 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5701 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5761 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5821 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5881 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5941 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6001 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6061 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6121 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6181 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6241 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6301 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6361 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6421 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 6481 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6541 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 6601 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6661 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6721 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 6781 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7621 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8941 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9001 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9061 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9121 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9181 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9241 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9301 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9361 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.