pLV3-CMV-STARD9-circRNA-CopGFP

pLV3-CMV-STARD9-circRNA-CopGFP-Puro plasmid

PVT96485

pLV3-CMV-STARD9-circRNA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-STARD9(human)-circRNA-CopGFP-Puro
Alias: -
Gene lenght: 325bp
Host: Mammalian cells,lentivirus
Use(s): Transposition regulation
Fragment type: circRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-STARD9-circRNA-CopGFP-Puro plasmid Description

pLV3-CMV-STARD9-circRNA-CopGFP-Puro A plasmid suitable for transcriptional regulation in mammalian cells, also applicable for lentivirus packaging.

pLV3-CMV-STARD9-circRNA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagatTA 2281 ATACTTTCAG aattcGCCAC CGTTTTCCAG GATTTAGGGA TGGAAGTACT GTCTGGAGTT 2341 GCCAAAGGCT ATAACATATG CCTTTTTGCT TATGGACAGA CAGGCTCTGG GAAGACATAT 2401 ACCATGCTGG GGACCCCAGC CTCTGTTGGG TTGACACCAC GGATATGTGA GGGTCTCTTC 2461 GTCAGGGAGA AAGACTGTGC CTCACTGCCT TCCTCCTGTA GGATAAAAGT AAGTTTTCTA 2521 GAAATCTATA ATGAACGGGT GCGGGATCTG TTGAAGCAAT CTGGTCAAAA AAAGTCCTAT 2581 ACCCTGCGGG TCAGGGAGCA TCCAGAGATG GGGCCCTATG TACAAGCTCG AGTAAGAACA 2641 ACTGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 2701 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg 2761 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 2821 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 2881 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct 2941 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct 3001 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg 3061 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg 3121 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac 3181 cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga 3241 tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag 3301 agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga 3361 ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct 3421 accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca 3481 cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg 3541 aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg 3601 tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg 3661 gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact 3721 acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc 3781 agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc 3841 tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg 3901 ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg 3961 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg 4021 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 4081 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 4141 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 4201 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 4261 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 4321 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 4381 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 4441 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 4501 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 4561 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac 4621 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 4681 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 4741 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 4801 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 4861 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 4921 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 4981 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 5041 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 5101 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc 5161 gccctcagac gagtcggatc tccctttggc cgcctccccg cctggtacct ttaagaccaa 5221 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 5281 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 5341 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 5401 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 5461 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 5521 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 5581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5701 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 5761 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 5821 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 5881 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 5941 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6001 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6061 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6721 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 6901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 6961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7021 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 7381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 7441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 7681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 7741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 7801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 7861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 7981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8041 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 8101 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 8161 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 8221 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 8281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 8341 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 8401 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 8461 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.