pLX304 Luciferase-EGFP-V5 blas

pLX304 Luciferase-EGFP-V5 blast plasmid

PVT96471

pLX304 Luciferase-EGFP-V5 blast plasmid Information

Plasmid name: pLX304 Luciferase-EGFP-V5 blast
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: Luc-F
Reverse primer:
Copy number: High
Induction:

pLX304 Luciferase-EGFP-V5 blast plasmid Description

pLX304 Luciferase-EGFP-V5 blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLX304 Luciferase-EGFP-V5 blast plasmid Sequence

1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 601 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 661 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta 721 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg 781 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt 841 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac 901 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 961 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 1021 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 1081 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc 1141 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga 1201 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca 1261 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa 1321 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag 1381 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac 1441 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct 1501 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg 1561 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga 1621 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt 1681 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga 1741 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc 1801 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag 1861 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg 1921 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag 1981 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat 2041 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg 2101 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac 2161 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat 2221 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc 2281 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga 2341 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc 2401 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc 2461 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc 2521 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa 2581 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt 2641 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg 2701 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg 2761 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc 2821 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc 2881 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg 2941 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg 3001 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg 3061 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga 3121 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc 3181 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac 3241 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc 3301 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta 3361 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa 3421 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt 3481 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat 3541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 3601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 3661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 3721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 3781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 3841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 3901 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 3961 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 4021 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 4081 tctatataag cagagctctc tggctaactg tcgggatcaa caagtttgta caaaaaagtt 4141 ggcaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa 4201 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga 4261 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa 4321 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc 4381 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc 4441 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg 4501 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac 4561 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac 4621 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa 4681 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc 4741 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga 4801 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta 4861 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt 4921 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag 4981 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc 5041 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc 5101 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa 5161 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa 5221 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc 5281 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg 5341 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta 5401 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg 5461 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg 5521 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt 5581 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc 5641 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt 5701 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc 5761 ctcataaagg ccaagaaggg cggaaagatc gccgtgttgc caactttctt gtacaaagtg 5821 gttGGCGGTG GAGGAAGCgg cggaggtgga tctatggtga gcaagggcga ggagctgttc 5881 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 5941 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 6001 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 6061 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 6121 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 6181 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 6241 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 6301 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 6361 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 6421 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 6481 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 6541 atcactctcg gcatggacga gctgtacaag ggtaagccta tccctaaccc tctcctcggt 6601 ctcgattcta cgtagtaatg agctagcacc ggtggcgcgt taagtcgaca atcaacctct 6661 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6721 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6781 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6841 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6901 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 6961 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 7021 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 7081 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 7141 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 7201 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact 7261 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 7321 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 7381 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 7441 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 7501 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca 7561 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 7621 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 7681 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 7741 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 7801 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 7861 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 7921 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 7981 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 8041 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 8101 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 8161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 8221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 8281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 8341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 8401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 8461 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 8521 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 8581 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 8641 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 8701 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 8761 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 8821 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 8881 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 8941 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 9001 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 9061 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 9121 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 9181 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 9241 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 9301 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 9361 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 9421 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaaaccctc ccgtatcgta 9481 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 9541 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 9601 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 9661 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 9721 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 9781 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 9841 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 9901 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 9961 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 10021 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 10081 cccagcttgg agcgaacgac ctacaccgaa ctgagata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.