pLV3-CMV-Mito-Ogg1-3×FLAG-CopG

pLV3-CMV-Mito-Ogg1-3×FLAG-CopGFP-Puro plasmid

PVT96469

pLV3-CMV-Mito-Ogg1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Mito-Ogg1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_010957.4;Mmh
Gene lenght: 1035bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mito-Ogg1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Mito-Ogg1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Mito-Ogg1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc 2341 ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc cCatgttatt 2401 ccgttcctgg ctgcctagca gcatgagaca tcgcacccta agctccagcc cggccctctg 2461 ggcctctatc ccgtgtccac gctctgagct gcgtctggac ttggttttag cttctggaca 2521 gtccttccgg tggaaggagc agagccctgc tcactggagt ggcgtgctgg cagatcaagt 2581 atggacactg actcagacgg aggaccagct ctattgcact gtgtaccgag gagacgacag 2641 ccaggtcagc aggcccaccc tagaggagct ggaaacccta cacaagtact ttcagctaga 2701 tgtcagcctg gcacagctgt atagccactg ggcttctgta gactcccact tccaaagagt 2761 ggcccagaaa ttccaaggtg tgagactgct gagacaagac cccactgagt gccttttctc 2821 tttcatctgt tcctccaaca acaacattgc tcgcattact ggcatggtgg aacggctctg 2881 ccaggccttt ggacctcgac tcattcagct tgatgatgtc acttatcatg gcttcccaaa 2941 cctccatgcc ctggctggtc cagaagcaga gactcacctg aggaagttgg gcctggggta 3001 ccgtgcccgc tatgtacgtg ccagtgccaa agccatcctc gaagagcaag gtgggccagc 3061 ttggctgcag cagcttcgag tggcccctta tgaagaggcc cacaaggccc tctgcaccct 3121 gcccggggtg ggcgccaagg tggctgactg catctgctta atggcccttg acaaacccca 3181 ggctgtgccc gtggatgtcc atgtatggca gattgcccat cgtgactacg gctggcatcc 3241 taagacatcc caggctaagg gcccgagccc tttggccaac aaagaactgg gaaacttttt 3301 ccggaatctg tggggacctt atgctggctg ggcccaagca gtgctgttca gtgctgacct 3361 tcgccaacca agcctgtctc gggagccacc agcaaagcgc aaaaagggat ctaagaggcc 3421 agagggcccg gtcgccaccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT 3481 CGACTACAAG GATGACGATG ACAAGTGAgc ggccgcGaag gatctgcgat cgctccggtg 3541 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 3601 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 3661 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 3721 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc 3781 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc 3841 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt 3901 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca 3961 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt 4021 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg 4081 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga 4141 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga 4201 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg 4261 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc 4321 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg 4381 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca 4441 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca 4501 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg 4561 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc 4621 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc 4681 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct 4741 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg 4801 gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg 4861 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca 4921 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 4981 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 5041 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 5101 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 5161 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 5221 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 5281 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 5341 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 5401 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 5461 gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat 5521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5881 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6001 cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt 6061 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta 6121 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag 6181 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 6241 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 6301 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 6361 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 6421 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 6481 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 6541 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 6601 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 6661 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 6721 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc 6781 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 6841 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 6901 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 6961 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 7021 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7081 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 7141 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 7201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7261 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 7381 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 7441 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 7501 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 7561 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 7621 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 7681 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 7741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 7801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7921 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7981 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8041 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 8101 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 8161 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 8221 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8281 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 8341 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 8401 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 8461 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 8521 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 8581 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 8641 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 8701 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 8761 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 8821 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8881 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 8941 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 9001 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 9061 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 9121 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 9181 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 9241 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 9301 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 9361 acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.