pLV3-CMV-SLCO4A1-AS1-EF1a-CopG

pLV3-CMV-SLCO4A1-AS1-EF1a-CopGFP-Puro plasmid

PVT96426

pLV3-CMV-SLCO4A1-AS1-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SLCO4A1-AS1(human)-EF1a-CopGFP-Puro
Alias: NR_024470.1
Gene lenght: 1440bp
Host: Mammalian cells,lentivirus
Use(s): RNATranscription
Fragment type: ncRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLCO4A1-AS1-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-SLCO4A1-AS1-EF1a-CopGFP-Puro A plasmid suitable for transcriptional regulation in mammalian cells, also applicable for lentivirus packaging.

pLV3-CMV-SLCO4A1-AS1-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc ggtgcttggg gccccgacgc gtacccaggt 2341 gccatctggc cctcacctcc cgccgtagct ggctgtgacg cccgccatgg gcacactggg 2401 gcagtgcagt gagaagacga ggatgcccag caggctgaca acggtgcaga acaggcagaa 2461 cttgatgacc gcggagcccc ggagcctgag cttgttcaca aagaagccgc ccaggaaggt 2521 gccgccacca cccgctggca ccaccagggc agccacaccc cctcctcccc caggggcctc 2581 tgccgagtcc ctgcaggctg tgacaaggac atttgtcgaa gcctgagtgg ccaccaacag 2641 taaaaaattg cgattagctg gcatctagct tctgggctga caagtgagga gggcacagac 2701 ctgcccagcc tgggaaggcc agcctgcctc tggcaccgag aaacccccag ctgcactgtt 2761 ccaggaggtc ctgaggcacc agtgcctggc tcatggaaca gccaggtcca aacccttcct 2821 gcaccaagtc acagaagcca agactaggtg gctagggggt ggagcccacc gtggaccctt 2881 ccacctacgg agcatgtccc acaaagaggg caggaccacc actctcccgg cctgtggaca 2941 gagtcgctca gcccccgtgg cgctctccac acctgctctg gcagcggggg tgggagggca 3001 ctcactgcca tgtgccctgt ctgggtcttt ctagaagatg cccttgaaga ctctgtttct 3061 gagaactgac ataaaagcag aggaccccca agattcagtt ttctcaccaa acaaggtggc 3121 agcttctgag gcactcaggc tgaactggga ctccaagaac ttggggctga acgtggacat 3181 gccggtgatg agagtggcct cggtggcccc ggccaggcag agcaggatga acgtggggtt 3241 cttcagcagg agccagatgg agctgggcag agtgtcgctg acttgaaccc ctgcgtctgc 3301 agcttagtga catcctgtaa acctacactt tccagcctct caccagagca gactgtcggc 3361 ctcacatcac ccccacctgc aggagggcgg ctctttcctc tcggccacac ctagagcctg 3421 gttccgatga acgcaactct gaatgcctgg aacattcaaa tgctcttgtt tgaggaggtg 3481 gccaaatgta aatggattct gaagaatcag gaacagacgg tgcagcccta gttagcctct 3541 gagccccaac aggcccacag gtgccttgca gagctgatgt tggcgtcaga aactccgctc 3601 cagcctcaca ggcactctgc ctgcttggag gtgttcgctg gctcgctcag actgacctca 3661 gcagccatga acatgggttc tttctgtccc cttccctgat taaaatggga gagcccagtt 3721 ctttcaaaat ttaaaaaaaa aaaaaaaaaa ggatccCTGC GATATCCAGC gcggccgcGa 3781 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 3841 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 3901 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 3961 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 4021 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 4081 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4141 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 4201 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 4261 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 4321 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 4381 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 4441 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 4501 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 4561 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 4621 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 4681 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 4741 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 4801 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 4861 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 4921 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 4981 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 5041 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 5101 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 5161 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5221 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5281 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5341 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5401 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5461 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5521 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5581 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5641 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5701 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 5761 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5821 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5881 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5941 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6001 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6061 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6121 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 6181 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6241 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct 6301 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact 6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 6421 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac 6481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 6661 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 6721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 6781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 6841 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 6901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 6961 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 7021 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 7081 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 7141 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 7201 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 7261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 7321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 7381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 7441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 7501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 7561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 7621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 7681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 7741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 7861 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 7921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 7981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 8041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 8101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 8161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 8221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 8281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 8341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 8401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 8461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 8521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 8581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 8641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 8701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 8761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 8821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 8881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 8941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 9001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 9061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 9121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 9181 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 9241 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 9301 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 9361 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 9421 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 9481 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 9541 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 9601 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.