pLV3-CMV-MYH9-3×FLAG-CopGFP-Pu

pLV3-CMV-MYH9-3×FLAG-CopGFP-Puro plasmid

PVT96421

pLV3-CMV-MYH9-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MYH9(human)-3×FLAG-CopGFP-Puro
Alias: NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA;NMHC-II-A; NMMHC-IIA; NMMHCA
Gene lenght: 5880bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MYH9-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MYH9-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MYH9-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cacagcaagc tgccgataag 2341 tatctctatg tggataaaaa cttcatcaac aatccgctgg cccaggccga ctgggctgcc 2401 aagaagctgg tatgggtgcc ttccgacaag agtggctttg agccagccag cctcaaggag 2461 gaggtgggcg aagaggccat cgtggagctg gtggagaatg ggaagaaggt gaaggtgaac 2521 aaggatgaca tccagaagat gaacccgccc aagttctcca aggtggagga catggcagag 2581 ctcacgtgcc tcaacgaagc ctcggtgctg cacaacctca aggagcgtta ctactcaggg 2641 ctcatctaca cctattcagg cctgttctgt gtggtcatca atccttacaa gaacctgccc 2701 atctactctg aagagattgt ggaaatgtac aagggcaaga agaggcacga gatgccccct 2761 cacatctatg ccatcacaga caccgcctac aggagtatga tgcaagaccg agaagatcaa 2821 tccatcttgt gcactggtga atctggagct ggcaagacgg agaacaccaa gaaggtcatc 2881 cagtatctgg cgtacgtggc gtcctcgcac aagagcaaga aggaccaggg cgagctggag 2941 cggcagctgc tgcaggccaa ccccatcctg gaggccttcg ggaacgccaa gaccgtgaag 3001 aatgacaact cctcccgctt cggcaaattc attcgcatca actttgatgt caatggctac 3061 attgttggag ccaacattga gacttatctt ttggagaaat ctcgtgctat ccgccaagcc 3121 aaggaagaac ggaccttcca catcttctat tatctcctgt ctggggctgg agagcacctg 3181 aagaccgatc tcctgttgga gccgtacaac aaataccgct tcctgtccaa tggacacgtc 3241 accatccccg ggcagcagga caaggacatg ttccaggaga ccatggaggc catgaggatt 3301 atgggcatcc cagaagagga gcaaatgggc ctgctgcggg tcatctcagg ggttcttcag 3361 ctcggcaaca tcgtcttcaa gaaggagcgg aacactgacc aggcgtccat gcccgacaac 3421 acagctgccc aaaaggtgtc ccatctcttg ggtatcaatg tgaccgattt caccagagga 3481 atcctcaccc cgcgcatcaa ggtgggacgg gattacgtcc agaaggcgca gactaaagag 3541 caggctgact ttgccatcga ggccttggcc aaggcgacct atgagcggat gttccgctgg 3601 ctggtgctgc gcatcaacaa ggctctggac aagaccaaga ggcagggcgc ctccttcatc 3661 gggatcctgg acattgccgg cttcgagatc tttgatctga actcgtttga gcagctgtgc 3721 atcaattaca ccaatgagaa gctgcagcag ctcttcaacc acaccatgtt catcctggag 3781 caggaggagt accagcgcga gggcatcgag tggaacttca tcgactttgg cctcgacctg 3841 cagccctgca tcgacctcat tgagaagcca gcaggccccc cgggcattct ggccctgctg 3901 gacgaggagt gctggttccc caaagccacc gacaagagct tcgtggagaa ggtgatgcag 3961 gagcagggca cccaccccaa gttccagaag cccaagcagc tgaaggacaa agctgatttc 4021 tgcattatcc actatgccgg caaggtggat tacaaagctg acgagtggct gatgaagaac 4081 atggatcccc tgaatgacaa catcgccaca ctgctccacc agtcctctga caagtttgtc 4141 tcggagctgt ggaaggatgt ggaccgcatc atcggcctgg accaggtggc cggcatgtcg 4201 gagaccgcac tgcccggggc cttcaagacg cggaagggca tgttccgcac tgtggggcag 4261 ctttacaagg agcagctggc caagctgatg gctacgctga ggaacacgaa ccccaacttt 4321 gtccgctgca tcatccccaa ccacgagaag aaggccggca agctggaccc gcatctcgtg 4381 ctggaccagc tgcgctgcaa cggtgttctc gagggcatcc gtatctgccg ccagggcttc 4441 cccaacaggg tggtcttcca ggagtttcgg cagagatatg agatcctgac tccaaactcc 4501 attcccaagg gtttcatgga cgggaagcag gcgtgcgtgc tcatgataaa agccctggag 4561 ctcgacagca atctgtaccg cattggccag agcaaagtct tcttccgtgc cggtgtgctg 4621 gcccacctgg aggaggagcg agacctgaag atcaccgacg tcatcatagg gttccaggcc 4681 tgctgcaggg gctacctggc caggaaagca tttgccaagc ggcagcagca gcttaccgcc 4741 atgaaggtcc tccagcggaa ctgcgctgcc tacctgaagc tgcggaactg gcagtggtgg 4801 cggctcttca ccaaggtcaa gccgctgctg caggtgagcc ggcaggagga ggagatgatg 4861 gccaaggagg aggagctggt gaaggtcaga gagaagcagc tggctgcgga gaacaggctc 4921 acggagatgg agacgctgca gtctcagctc atggcagaga aattgcagct gcaggagcag 4981 ctccaggcag aaaccgagct gtgtgccgag gctgaggagc tccgggcccg cctgaccgcc 5041 aagaagcagg aattagaaga gatctgccat gacctagagg ccagggtgga ggaggaggag 5101 gagcgctgcc agcacctgca ggcggagaag aagaagatgc agcagaacat ccaggagctt 5161 gaggagcagc tggaggagga ggagagcgcc cggcagaagc tgcagctgga gaaggtgacc 5221 accgaggcga agctgaaaaa gctggaggag gagcagatca tcctggagga ccagaactgc 5281 aagctggcca aggaaaagaa actgctggaa gacagaatag ctgagttcac caccaacctc 5341 acagaagagg aggagaaatc taagagcctc gccaagctca agaacaagca tgaggcaatg 5401 atcactgact tggaagagcg cctccgcagg gaggagaagc agcgacagga gctggagaag 5461 acccgccgga agctggaggg agactccaca gacctcagcg accagatcgc cgagctccag 5521 gcccagatcg cggagctcaa gatgcagctg gccaagaaag aggaggagct ccaggccgcc 5581 ctggccagag tggaagagga agctgcccag aagaacatgg ccctcaagaa gatccgggag 5641 ctggaatctc agatctctga actccaggaa gacctggagt ctgagcgtgc ttccaggaat 5701 aaagctgaga agcagaaacg ggaccttggg gaagagctag aggctctgaa aacagagttg 5761 gaggacacgc tggattccac agctgcccag caggagctca ggtcaaaacg tgagcaggag 5821 gtgaacatcc tgaagaagac cctggaggag gaggccaaga cccacgaggc ccagatccag 5881 gagatgaggc agaagcactc acaggccgtg gaggagctgg cggagcagct ggagcagacg 5941 aagcgggtga aagcaaacct cgagaaggca aagcagactc tggagaacga gcggggggag 6001 ctggccaacg aggtgaaggt gctgctgcag ggcaaagggg actcggagca caagcgcaag 6061 aaagtggagg cgcagctgca ggagctgcag gtcaagttca acgagggaga gcgcgtgcgc 6121 acagagctgg ccgacaaggt caccaagctg caggtggagc tggacaacgt gaccgggctt 6181 ctcagccagt ccgacagcaa gtccagcaag ctcaccaagg acttctccgc gctggagtcc 6241 cagctgcagg acactcagga gctgctgcag gaggagaacc ggcagaagct gagcctgagc 6301 accaagctca agcaggtgga ggacgagaag aattccttcc gggagcagct ggaggaggag 6361 gaggaggcca agcacaacct ggagaagcag atcgccaccc tccatgccca ggtggccgac 6421 atgaaaaaga agatggagga cagtgtgggg tgcctggaaa ctgctgagga ggtgaagagg 6481 aagctccaga aggacctgga gggcctgagc cagcggcacg aggagaaggt ggccgcctac 6541 gacaagctgg agaagaccaa gacgcggctg cagcaggagc tggacgacct gctggtggac 6601 ctggaccacc agcgccagag cgcgtgcaac ctggagaaga agcagaagaa gtttgaccag 6661 ctcctggcgg aggagaagac catctctgcc aagtatgcag aggagcgcga ccgggctgag 6721 gcggaggccc gagagaagga gaccaaggct ctgtcgctgg cccgggccct ggaggaagcc 6781 atggagcaga aggcggagct ggagcggctc aacaagcagt tccgcacgga gatggaggac 6841 cttatgagct ccaaggatga tgtgggcaag agtgtccacg agctggagaa gtccaagcgg 6901 gccctagagc agcaggtgga ggagatgaag acgcagctgg aagagctgga ggacgagctg 6961 caggccaccg aagatgccaa gctgcggttg gaggtcaacc tgcaggccat gaaggcccag 7021 ttcgagcggg acctgcaggg ccgggacgag cagagcgagg agaagaagaa gcagctggtc 7081 agacaggtgc gggagatgga ggcagagctg gaggacgaga ggaagcagcg ctcgatggca 7141 gtggccgccc ggaagaagct ggagatggac ctgaaggacc tggaggcgca catcgactcg 7201 gccaacaaga accgggacga agccatcaaa cagctgcgga agctgcaggc ccagatgaag 7261 gactgcatgc gcgagctgga tgacacccgc gcctctcgtg aggagatcct ggcccaggcc 7321 aaagagaacg agaagaagct gaagagcatg gaggccgaga tgatccagtt gcaggaggaa 7381 ctggcagccg cggagcgtgc caagcgccag gcccagcagg agcgggatga gctggctgac 7441 gagatcgcca acagcagcgg caaaggagcc ctggcgttag aggagaagcg gcgtctggag 7501 gcccgcatcg cccagctgga ggaggagctg gaggaggagc agggcaacac ggagctgatc 7561 aacgaccggc tgaagaaggc caacctgcag atcgaccaga tcaacaccga cctgaacctg 7621 gagcgcagcc acgcccagaa gaacgagaat gctcggcagc agctggaacg ccagaacaag 7681 gagcttaagg tcaagctgca ggagatggag ggcactgtca agtccaagta caaggcctcc 7741 atcaccgccc tcgaggccaa gattgcacag ctggaggagc agctggacaa cgagaccaag 7801 gagcgccagg cagcctgcaa acaggtgcgt cggaccgaga agaagctgaa ggatgtgctg 7861 ctgcaggtgg atgacgagcg gaggaacgcc gagcagtaca aggaccaggc cgacaaggca 7921 tctacccgcc tgaagcagct caagcggcag ctggaggagg ccgaagagga ggcccagcgg 7981 gccaacgcct cccgccggaa actgcagcgc gagctggagg acgccactga gacggccgat 8041 gccatgaacc gcgaagtcag ctccctaaag aacaagctca ggcgcgggga cctgccgttt 8101 gtcgtgcccc gccgaatggc ccggaaaggc gccggggatg gctccgacga agaggtagat 8161 ggcaaagcgg atggggctga ggccaaacct gccgaaggat ccGACTACAA AGACCATGAC 8221 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGta gcggccgcGa 8281 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 8341 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 8401 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 8461 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 8521 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 8581 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 8641 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 8701 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 8761 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 8821 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 8881 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 8941 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 9001 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 9061 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 9121 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 9181 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 9241 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 9301 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 9361 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 9421 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 9481 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 9541 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 9601 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 9661 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 9721 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 9781 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 9841 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 9901 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 9961 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 10021 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 10081 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 10141 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 10201 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 10261 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 10321 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 10381 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 10441 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 10501 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 10561 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 10621 aattccgtgg tgttgtcggg gaaATCATCG TCCTTTCCTt ggctgctcgc ctgtgttgcc 10681 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 10741 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctTcg ccttcgccct 10801 cagacgagtc ggatctccct ttggGccgcc tccccgcctg gtacctttaa gaccaatgac 10861 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 10921 aattcactcc caacgaaGat aagatctgct ttttgcttgt actgggtctc tctggttaga 10981 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 11041 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 11101 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 11161 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 11221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 11281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 11341 tgtctggctc tagctaTCCC GCCCCTAACT CCGCCCAtcc cgcccctaac tccgcccagt 11401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 11461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 11521 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 11581 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 11641 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 11701 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 11761 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 11821 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 11881 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 11941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 12001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 12061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 12121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 12181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 12241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 12301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 12361 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 12421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 12481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 12541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 12601 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 12661 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 12721 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 12781 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 12841 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 12901 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 12961 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 13021 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 13081 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 13141 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 13201 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 13261 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 13321 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 13381 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 13441 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 13501 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 13561 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 13621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 13681 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 13741 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 13801 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 13861 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 13921 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 13981 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 14041 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 14101 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 14161 tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.