pLV3-CMV-EIF4A1-3×FLAG-Puro pl

pLV3-CMV-EIF4A1-3×FLAG-Puro plasmid

PVT96371

pLV3-CMV-EIF4A1-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-EIF4A1(human)-3×FLAG-Puro
Alias: NM_001416.4;DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I
Gene lenght: 1218bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EIF4A1-3×FLAG-Puro plasmid Description

pLV3-CMV-EIF4A1-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EIF4A1-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catgtctgcg agccaggatt cccgatccag agacaatggc 2341 cccgatggga tggagcccga aggcgtcatc gagagtaact ggaatgagat tgttgacagc 2401 tttgatgaca tgaacctctc ggagtccctt ctccgtggca tctacgcgta tggttttgag 2461 aagccctctg ccatccagca gcgagccatt ctaccttgta tcaagggtta tgatgtgatt 2521 gctcaagccc aatctgggac tgggaaaacg gccacatttg ccatatcgat tctgcagcag 2581 attgaattag atctaaaagc cacccaggcc ttggtcctag cacccactcg agaattggct 2641 cagcagatac agaaggtggt catggcacta ggagactaca tgggcgcctc ctgtcacgcc 2701 tgtatcgggg gcaccaacgt gcgtgctgag gtgcagaaac tgcagatgga agctccccac 2761 atcatcgtgg gtacccctgg ccgtgtgttt gatatgctta accggagata cctgtccccc 2821 aaatacatca agatgtttgt actggatgaa gctgacgaaa tgttaagccg tggattcaag 2881 gaccagatct atgacatatt ccaaaagctc aacagcaaca cccaggtagt tttgctgtca 2941 gccacaatgc cttctgatgt gcttgaggtg accaagaagt tcatgaggga ccccattcgg 3001 attcttgtca agaaggaaga gttgaccctg gagggtatcc gccagttcta catcaacgtg 3061 gaacgagagg agtggaagct ggacacacta tgtgacttgt atgaaaccct gaccatcacc 3121 caggcagtca tcttcatcaa cacccggagg aaggtggact ggctcaccga gaagatgcat 3181 gctcgagatt tcactgtatc cgccatgcat ggagatatgg accaaaagga acgagacgtg 3241 attatgaggg agtttcgttc tggctctagc agagttttga ttaccactga cctgctggcc 3301 agaggcattg atgtgcagca ggtttcttta gtcatcaact atgaccttcc caccaacagg 3361 gaaaactata tccacagaat cggtcgaggt ggacggtttg gccgtaaagg tgtggctatt 3421 aacatggtga cagaagaaga caagaggact cttcgagaca ttgagacctt ctacaacacc 3481 tccattgagg aaatgcccct caatgttgct gacctcatcG GATCCGACTA CAAAGACCAT 3541 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGCGGCCG 3601 CGAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC 3661 CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG 3721 GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA 3781 CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA 3841 ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA 3901 GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA 3961 ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG 4021 CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT 4081 CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG 4141 GCGCCTACGC TAGATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT 4201 CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC 4261 CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG 4321 CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG 4381 GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC 4441 CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA 4501 CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG 4561 CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT 4621 GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT 4681 CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA 4741 GCCCGGTGCC TGAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 4801 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 4861 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 4921 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 4981 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 5041 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 5101 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 5161 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 5221 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 5281 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 5341 CTCCCCGCCT GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT 5401 TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAAA TAAGATCTGC 5461 TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT 5521 AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT 5581 GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT 5641 GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA 5701 AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 5761 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 5821 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC 5881 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 5941 GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 6001 CCTAGACTTT TGCAGAGACG GCCCAAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG 6061 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 6121 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 6181 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 6241 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 6301 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 6361 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 6421 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 6481 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 6541 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 6601 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 6661 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 6721 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 6781 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 6841 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 6901 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 6961 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 7021 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 7081 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 7141 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 7201 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 7261 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 7321 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 7381 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 7441 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 7501 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 7561 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 7621 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 7681 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 7741 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 7801 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 7861 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 7921 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 7981 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 8041 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 8101 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 8161 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC 8221 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 8281 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 8341 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 8401 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 8461 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG 8521 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 8581 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 8641 TAAAACGACG GCCAGTGCCA AGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.