pLV3-CMV-PHB2-3×FLAG-mCherry-N

pLV3-CMV-PHB2-3×FLAG-mCherry-Neo plasmid

PVT96343

pLV3-CMV-PHB2-3×FLAG-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-PHB2(human)-3×FLAG-mCherry-Neo
Alias: NM_001144831.2;BAP; Bap37; BCAP37; hBAP; p22; PNAS-141;REA
Gene lenght: 897bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PHB2-3×FLAG-mCherry-Neo plasmid Description

pLV3-CMV-PHB2-3×FLAG-mCherry-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PHB2-3×FLAG-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggccca gaacttgaag gacttggcgg gacggctgcc 2341 cgccgggccc cggggcatgg gcacggccct gaagctgttg ctgggggccg gcgccgtggc 2401 ctacggtgtg cgcgaatctg tgttcaccgt ggaaggcggg cacagagcca tcttcttcaa 2461 tcggatcggt ggagtgcagc aggacactat cctggccgag ggccttcact tcaggatccc 2521 ttggttccag taccccatta tctatgacat tcgggccaga cctcgaaaaa tctcctcccc 2581 tacaggctcc aaagacctac agatggtgaa tatctccctg cgagtgttgt ctcgacccaa 2641 tgctcaggag cttcctagca tgtaccagcg cctagggctg gactacgagg aacgagtgtt 2701 gccgtccatt gtcaacgagg tgctcaagag tgtggtggcc aagttcaatg cctcacagct 2761 gatcacccag cgggcccagg tatccctgtt gatccgccgg gagctgacag agagggccaa 2821 ggacttcagc ctcatcctgg atgatgtggc catcacagag ctgagcttta gccgagagta 2881 cacagctgct gtagaagcca aacaagtggc ccagcaggag gcccagcggg cccaattctt 2941 ggtagaaaaa gcaaagcagg aacagcggca gaaaattgtg caggccgagg gtgaggccga 3001 ggctgccaag atgcttggag aagcactgag caagaaccct ggctacatca aacttcgcaa 3061 gattcgagca gcccagaata tctccaagac gatcgccaca tcacagaatc gtatctatct 3121 cacagctgac aaccttgtgc tgaacctaca ggatgaaagt ttcaccaggg gaagtgacag 3181 cctcatcaag ggtaagaaag gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 3241 TGACATCGAC TACAAGGATG ACGATGACAA GTAgcggccg cGaaggatct gcgatcgctc 3301 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3361 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 3421 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 3481 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 3541 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 3601 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 3661 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 3721 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 3781 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 3841 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 3901 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 3961 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 4021 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 4081 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 4141 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 4201 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 4261 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 4321 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 4381 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 4441 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 4501 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctcgcga 4561 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctGCCACCat 4621 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4681 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4741 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4801 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4861 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4921 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4981 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 5041 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 5101 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 5161 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 5221 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 5281 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 5341 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 5401 cgagttcttc tgaGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 5461 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 5521 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 5581 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 5641 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 5701 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 5761 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 5821 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 5881 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 5941 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 6001 CTCCCCGCgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6061 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata agatctgctt 6121 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 6181 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 6241 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 6301 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 6361 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 6421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 6481 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 6541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 6601 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 6661 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 6721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 6781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 6841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 6901 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 7441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 7501 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 7561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 7621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 7681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 7741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 7801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7981 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 8401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 8461 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 8521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 8581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 8641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 8701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 8761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8821 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 8881 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 8941 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 9001 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 9061 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 9121 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 9181 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 9241 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 9301 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.