pLV3-U6-ANGPTL1-shRNA1-CopGFP-

pLV3-U6-ANGPTL1-shRNA1-CopGFP-Puro plasmid

PVT96303

pLV3-U6-ANGPTL1-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-ANGPTL1(human)-shRNA1-CopGFP-Puro
Alias: ggagatagatgttctgcaact
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-ANGPTL1-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-ANGPTL1-shRNA1-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-ANGPTL1-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg gagatagatg ttctgcaact TTCAAGAGAa 2221 gttgcagaac atctatctcc ttttttggat ccgcggccgc Gaaggatctg cgatcgctcc 2281 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 2341 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 2401 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 2461 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 2521 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 2581 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 2641 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 2701 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 2761 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 2821 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 2881 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 2941 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3001 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3061 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3121 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 3181 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 3241 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 3301 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 3361 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 3421 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 3481 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 3541 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 3601 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 3661 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 3721 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 3781 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 3841 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 3901 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 3961 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 4021 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 4081 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 4141 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 4201 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 4261 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 4321 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 4381 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 4441 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 4501 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 4561 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 4621 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 4681 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 4741 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc 4801 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat 4861 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa 4921 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 4981 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 5041 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 5101 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 5161 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 5221 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 5281 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 5341 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5401 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5461 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt 5521 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5581 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5641 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5701 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5761 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 5821 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 5881 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5941 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6001 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6061 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6121 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6181 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6241 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6301 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 6361 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6421 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 6481 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6541 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6601 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6661 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6721 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6781 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6841 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6901 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6961 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7021 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7081 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7141 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7201 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7261 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7321 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7381 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7441 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7501 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7561 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7621 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 7681 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 7741 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 7801 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 7861 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 7921 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 7981 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 8041 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 8101 cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.