pLV3-CMV-Cbx2-3×FLAG-EF1a-mChe

pLV3-CMV-Cbx2-3×FLAG-EF1a-mCherry-Blast plasmid

PVT96291

pLV3-CMV-Cbx2-3×FLAG-EF1a-mCherry-Blast plasmid Information

Plasmid name: pLV3-CMV-Cbx2(mouse)-3×FLAG-EF1a-mCherry-Blast
Alias: NM_007623.3;M33; MOD2; pc;
Gene lenght: 1557bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cbx2-3×FLAG-EF1a-mCherry-Blast plasmid Description

pLV3-CMV-Cbx2-3×FLAG-EF1a-mCherry-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Cbx2-3×FLAG-EF1a-mCherry-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggagga gctgagcagc gtgggcgagc aggtcttcgc 2341 cgccgagtgc atcctgagca agcggctccg caagggcaag ctggagtacc tggtcaagtg 2401 gcgcggctgg tcctccaaac acaacagctg ggagccagaa gagaacattt tggacccgag 2461 gctgctccta gccttccaga agaaggaaca tgagaaggag gttcagaacc ggaagagagg 2521 caagagaccc aggggcaggc cgaggaaaca cacagtcaca tcctcctgca gccggcgctc 2581 caagctcaag gaaccagatg cgccatccaa atccaaatcc agcagttcgt cctcttcctc 2641 cacatcttcc tcctcttcct cggacgaaga ggaagacgac agcgacctag actccaagag 2701 aggcccccgg ggccgtgaaa cccatccagt gcctcagaaa aaagcccaga tcctggtagc 2761 caagccagag ctgaaggatc ccattagaaa gaaacgggga cgcaagcctc tacccccaga 2821 acagaaggca gctcggagac ccgtcagcct ggccaaggtg ctaaagacca ccaggaagga 2881 tctggggacc tcagccgcca agctgccccc tccactcagc gctccggtgg caggcctggc 2941 tgccctgaag gcccacacca aagaggcctg tggtggcccc agcactatgg cgaccccaga 3001 gaacctggcc agtctgatga aaggcatggc cgggagcccc agcagaggcg gcatctggca 3061 gagctccatc gtacactaca tgaaccgcat gagccagagt caggttcagg ctgccagccg 3121 actggcactc aaggcccagg ccaccaacaa gtgcggtctc gggctagacc tgaaagtgag 3181 gacgcagaag gggggtgagc taggggggag ccccgcagga ggcaaggtcc cgaaggcccc 3241 cggtggcgga gctgcagagc agcagagagg gaaccattcg gggagcccag gtgctcagct 3301 ggcacccact caggagttga gccttcaggt ccttgacttg caaagcgtca agaacggtgt 3361 gcctggtgtg ggcctgcttg ctcgccatgc cccagccaag gctattcctg ctaccaaccc 3421 agccacaggg aaaggtcctg ggagcggccc cacaggagca aacatgacca acgctcccac 3481 agacaacaac aaaggggaaa agctgacttg caaggcaacg gctctgcctg ccccttccgt 3541 caagcgggac accgttaaaa gcgtcgctgc ctccggcggg caggagggcc acacagcccc 3601 gggagaaggc cgaaagccac ctgcgctgtc tgagctgagc acgggagagg agaatagtag 3661 ctctgactcg gaccctgact cgacctcgct tcccagtgct gcgcagaacc tatctgtagc 3721 tatccagacc agccaggact ggaaacctac ccgcagtctc atcgaacacg tctttgtcac 3781 ggatgtcaca gccaacctca tcactgtcac cgtgaaggag tcgcccacca gcgtgggctt 3841 cttcaacttg aggcattatg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 3901 TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc 3961 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 4021 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 4081 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 4141 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 4201 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 4261 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 4321 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 4381 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 4441 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 4501 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 4561 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 4621 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 4681 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 4741 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 4801 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 4861 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 4921 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 4981 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 5041 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 5101 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 5161 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctcgcga 5221 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctATGGCCAA 5281 GCCTTTGTCT CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT 5341 CCCCATCTCT GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT 5401 CACTGGTGTC AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG 5461 CACTGCTGCT GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA 5521 CAGGGGCATC TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG 5581 GATCAAAGCC ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT 5641 GCTGCCCTCT GGTTATGTGT GGGAGGGCTA AGTCGACaat caacctctgg attacaaaat 5701 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5761 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5821 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5881 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5941 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 6001 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 6061 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 6121 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 6181 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg 6241 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6301 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6361 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6421 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6481 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6541 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6601 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6781 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6901 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 6961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 7021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 7081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 7141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 8041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 8101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 9001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 9061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 9121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 9181 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 9241 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 9301 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 9361 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 9421 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 9481 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 9541 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.