p35S-FLAG-Citrine-35S-EGFP-Neo

p35S-FLAG-Citrine-35S-EGFP-Neo plasmid

PVT96260

p35S-FLAG-Citrine-35S-EGFP-Neo plasmid Information

Plasmid name: p35S-FLAG-Citrine-35S-EGFP-Neo
Alias: -
Gene lenght: 916bp
Host: Plant
Use(s): Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green,Yellow
Promoter: 35S
Replicon: pVS1
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F
Reverse primer:
Copy number: High
Induction:

p35S-FLAG-Citrine-35S-EGFP-Neo plasmid Description

p35S-FLAG-Citrine-35S-EGFP-Neo This is a plasmid suitable for protein expression in plants.

p35S-FLAG-Citrine-35S-EGFP-Neo plasmid Sequence

1 CCAGCCAGCC AACAGCTCCC CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC 61 AGCCCATCAG TCCGGGACGG CGTCAGCGGG AGAGCCGTTG TAAGGCGGCA GACTTTGCTC 121 ATGTTACCGA TGCTATTCGG AAGAACGGCA ACTAAGCTGC CGGGTTTGAA ACACGGATGA 181 TCTCGCGGAG GGTAGCATGT TGATTGTAAC GATGACAGAG CGTTGCTGCC TGTGATCACC 241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg 301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg 361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata 421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg 481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga 541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg 601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt 661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc 721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat 781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg 841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg 901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa 961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa 1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta 1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga 1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt 1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc 1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct 2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga 2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg 2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg 2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc 2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt 2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg 2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc 2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat 2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg 2761 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt 2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat 2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat 2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta 3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta 3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc 3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta 3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc 3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt 3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg 3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc 3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag 3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc 3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac 3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa 3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg 3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg 3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag 3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg 3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta 3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt 4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat 4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg 4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg 4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt 4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat 4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt 4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca 4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa 4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc 4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata 4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca 4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg 4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt 4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat 4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc 4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt 4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca 5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc 5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc 5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa 5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc 5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc 5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga 5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg 5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg 5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg 5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac 5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt 5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg 5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc 5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc 5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg 5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca 6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac 6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg 6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca 6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc 6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa 6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc 6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 6481 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgacGCAATg cagcttgccT 6541 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca 6601 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga 6661 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc 6721 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt 6781 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc 6841 acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc tcgtctactc 6901 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag 6961 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag 7021 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat 7081 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 7141 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc 7201 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccCtt cctctatata 7261 aggaagttca tttcatttgg agaggacacg ctgaCCCTGG AGACCCACGC TGAAATCACC 7321 AGTCTCTCTC TACAAATCTA TCTCTCTCGA GCTTTCGCAC CATGGACTAC AAAGACGATG 7381 ACGACAAGCA TTTAAATCTC GAGGGATCCT CTAGAAGATC CATCGCCACC ATGGTGAGCA 7441 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA 7501 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA 7561 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA 7621 CCTTCGGCTA CGGCCTGATG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT 7681 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CATCATCTTC TTCAAGGACG 7741 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA 7801 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT 7861 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG 7921 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC 7981 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT 8041 ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT 8101 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA TAGAGCTCGA 8161 ATTTCCCCGA TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG 8221 GTCTTGCGAT GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA 8281 TGTAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA 8341 TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG 8401 TGTCATCTAT GTTACTAGAT CGGGAATTCG TAATCATGTC ATAGCTGTTT CCTGTGTGAA 8461 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 8521 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 8581 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 8641 GTTTGCGTAT TGGCTAGAGC AGCTTGCCAA CATGGTGGAG CACGACACTC TCGTCTACTC 8701 CAAGAATATC AAAGATACAG TCTCAGAAGA CCAAAGGGCT ATTGAGACTT TTCAACAAAG 8761 GGTAATATCG GGAAACCTCC TCGGATTCCA TTGCCCAGCT ATCTGTCACT TCATCAAAAG 8821 GACAGTAGAA AAGGAAGGTG GCACCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCTAT 8881 CGTTCAAGAT GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT 8941 CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT GTGATAACAT 9001 GGTGGAGCAC GACACTCTCG TCTACTCCAA GAATATCAAA GATACAGTCT CAGAAGACCA 9061 AAGGGCTATT GAGACTTTTC AACAAAGGGT AATATCGGGA AACCTCCTCG GATTCCATTG 9121 CCCAGCTATC TGTCACTTCA TCAAAAGGAC AGTAGAAAAG GAAGGTGGCA CCTACAAATG 9181 CCATCATTGC GATAAAGGAA AGGCTATCGT TCAAGATGCC TCTGCCGACA GTGGTCCCAA 9241 AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC 9301 AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA 9361 TCCTTCGCAA GACCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG GACACGCTGA 9421 AATCACCAGT CTCTCTCTAC AAATCTATCT CTCTCGAGCT TTCGCAGATC TGTCGATCGA 9481 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG 9541 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT 9601 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA 9661 CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA 9721 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT 9781 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 9841 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC 9901 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA 9961 ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG 10021 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC 10081 ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG 10141 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGC 10201 ACCACCATCA CCATGCCATG GGGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG 10261 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG 10321 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT 10381 CCGGTGCCCT GAATGAACTC CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG 10441 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT 10501 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT 10561 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG 10621 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG 10681 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC 10741 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC ACATGGCGAT GCCTGCTTGC 10801 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG 10861 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG 10921 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA 10981 TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGGATCGAT 11041 CCTCTAGCTA GAGTCGATCG ACAAGCTCGA GTTTCTCCAT AATAATGTGT GAGTAGTTCC 11101 CAGATAAGGG AATTAGGGTT CCTATAGGGT TTCGCTCATG TGTTGAGCAT ATAAGAAACC 11161 CTTAGTATGT ATTTGTATTT GTAAAATACT TCTATCAATA AAATTTCTAA TTCCTAAAAC 11221 CAAAATCCAG TACTAAAATC CAGATCCCCC GAATTAATTC GGCGTTAATT CAGTACATTA 11281 AAAACGTCCG CAATGTGTTA TTAAGTTGTC TAAGCGTCAA TTTGTTTACA CCACAATATA 11341 TCCTGCCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.