pCAGGS-CXCR2-3×FLAG plasmid

pCAGGS-CXCR2-3×FLAG plasmid

PVT96249

pCAGGS-CXCR2-3×FLAG plasmid Information

Plasmid name: pCAGGS-CXCR2(monkey-opt-human)-3×FLAG
Alias: -
Gene lenght: 1065bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAGGS-CXCR2-3×FLAG plasmid Description

pCAGGS-CXCR2-3×FLAG is a plasmid suitable for protein expression in mammalian cells.

pCAGGS-CXCR2-3×FLAG plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGCCACC 1741 ATGGAGAGCT TCAATTTTGA GGACCTGTGG AAGGGCGAGG ATTTCAGCAA CTATTCCTAT 1801 TCCAGCGACC TGCCCCCATC CCTGCCTGAC GTGGCCCCCT GTAGACCGGA GTCTCTGGAA 1861 ATTAACAAGT ACTTCGTCGT GATCATCTAT GCACTGGTGT TTCTGCTGTC CCTGCTGGGC 1921 AACTCTCTGG TGATGCTGGT GATCCTGCAT AGCCGCGTGG GTCGGAGCAT CACCGATGTG 1981 TACCTGCTGA ACCTGGCCAT GGCCGACTTG CTGTTTGCTC TGACACTGCC CATCTGGGCT 2041 GCCGCCAAGG TGAACGGTTG GATTTTTGGC ACCTTCCTGT GTAAGGTGGT GAGCCTGCTC 2101 AAAGAAGTGA ACTTTTACAG TGGCATCCTG CTGCTCGCCT GTATCAGCGT GGACAGATAC 2161 CTGGCCATCG TGCACGCCAC ACGGACTCTG ACCCAGAAAA GGTACCTGGT GAAGTTCGTG 2221 TGTCTGTCAA TCTGGTCCCT GTCCCTGCTG CTGGCCCTGC CCGTGCTGCT GTTCCGCCGG 2281 ACCGTGTATC TGACCTACAT TTCCCCTGTG TGTTATGAGG ACATGGGCAA TAATACTGCC 2341 AAGTGGCGGA TGGTGCTGAG GATTCTGCCC CAGACCTTCG GCTTCATCCT GCCCCTGCTG 2401 ATTATGCTGT TCTGCTACGG TCTGACTCTG AGAACCCTGT TCAAGGCCCA CATGGGACAG 2461 AAGCATCGGG CCATGAGGGT GATATTCGCC GTGGTGCTGA TCTTTCTGCT GTGCTGGCTG 2521 CCATACCACC TGGTGCTGCT GGCCGACACC CTGATGAGAA CAAGACTGAT CAACGAGACC 2581 TGCCAGCGCC GCAACAATAT CGACCAGGCC CTGGACGCTA CAGAAATTCT GGGAATCCTC 2641 CACAGTTGTC TGAATCCCCT GATCTACGCT TTCATCGGCC AGAAATTTCG CCACGGCCTG 2701 CTGAAGATCC TGGCCACCCA CGGTCTGATC TCTAAAGACA GCCTGCCTAA GGACAGCCGC 2761 CCTTCCTTCG TGGGCAGCTC ATCAGGCCAC ACATCCACAA CCCTGGGTGG AGGCGGTAGC 2821 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGATA TCGATTACAA GGATGACGAT 2881 GACAAGTGAC TCGAGCTAGC AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG 2941 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG 3001 TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA 3061 CATCAGAATG AGTATTTGGT TTAGAGTTTG GCAACATATG CCCATATGCT GGCTGCCATG 3121 AACAAAGGTT GGCTATAAAG AGGTCATCAG TATATGAAAC AGCCCCCTGC TGTCCATTCC 3181 TTATTCCATA GAAAAGCCTT GACTTGAGGT TAGATTTTTT TTATATTTTG TTTTGTGTTA 3241 TTTTTTTCTT TAACATCCCT AAAATTTTCC TTACATGTTT TACTAGCCAG ATTTTTCCTC 3301 CTCTCCTGAC TACTCCCAGT CATAGCTGTC CCTCTTCTCT TATGGAGATC CCTCGACCTG 3361 CAGCCCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 3421 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG TGCCTAATGA 3481 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 3541 TCGTGCCAGC GGATCCGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC 3601 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 3661 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG 3721 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG CAGCTTATAA 3781 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 3841 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT 3901 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC 3961 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 4021 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGATAACGC AGGAAAGAAC ATGTGAGCAA 4081 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 4141 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 4201 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 4261 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 4321 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 4381 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 4441 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 4501 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 4561 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 4621 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 4681 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 4741 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 4801 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 4861 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 4921 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 4981 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 5041 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 5101 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 5161 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 5221 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 5281 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 5341 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 5401 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 5461 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 5521 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 5581 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 5641 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 5701 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 5761 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 5821 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 5881 G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.