pLV3-CMV-HLA-DMA-3×FLAG-Hyg pl

pLV3-CMV-HLA-DMA-3×FLAG-Hyg plasmid

PVT96177

pLV3-CMV-HLA-DMA-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-HLA-DMA(human)-3×FLAG-Hyg
Alias: NM_006120.4;D6S222E; DMA; HLADM; RING6
Gene lenght: 783bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HLA-DMA-3×FLAG-Hyg plasmid Description

pLV3-CMV-HLA-DMA-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HLA-DMA-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatgggtca tgaacagaac caaggagctg cgctgctaca 2341 gatgttacca cttctgtggc tgctacccca ctcctgggcc gtccctgaag ctcctactcc 2401 aatgtggcca gatgacctgc aaaaccacac attcctgcac acagtgtact gccaggatgg 2461 gagtcccagt gtgggactct ctgaggccta cgacgaggac cagcttttct tcttcgactt 2521 ttcccagaac actcgggtgc ctcgcctgcc cgaatttgct gactgggctc aggaacaggg 2581 agatgctcct gccattttat ttgacaaaga gttctgcgag tggatgatcc agcaaatagg 2641 gccaaaactt gatgggaaaa tcccggtgtc cagagggttt cctatcgctg aagtgttcac 2701 gctgaagccc ctggagtttg gcaagcccaa cactttggtc tgttttgtca gtaatctctt 2761 cccacccatg ctgacagtga actggcagca tcattccgtc cctgtggaag gatttgggcc 2821 tacttttgtc tcagctgtcg atggactcag cttccaggcc ttttcttact taaacttcac 2881 accagaacct tctgacattt tctcctgcat tgtgactcac gaaattgacc gctacacagc 2941 aattgcctat tgggtacccc ggaacgcact gccctcagat ctgctggaga atgtgctgtg 3001 tggcgtggcc tttggcctgg gtgtgctggg catcatcgtg ggcattgttc tcatcatcta 3061 cttccggaag ccttgctcag gtgacCTCGA GGACTACAAA GACCATGACG GTGATTATAA 3121 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG cggccgcaag gatctgcgat 3181 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 3241 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 3301 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 3361 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 3421 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 3481 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 3541 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 3601 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 3661 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgtcgaca 3721 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca 3781 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg 3841 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc 3901 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg 3961 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc 4021 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg 4081 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa 4141 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc 4201 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc 4261 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct 4321 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga 4381 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt 4441 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc 4501 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg 4561 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag 4621 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct 4681 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg 4741 aataggtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 4801 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 4861 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 4921 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 4981 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 5041 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 5101 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 5161 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 5221 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 5281 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 5341 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 5401 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 5461 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 5521 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 5581 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 5641 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 5701 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5821 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 5881 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 5941 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 6001 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6061 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6121 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6181 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 6241 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 6541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 6721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 6781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 6841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 6901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 6961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7501 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 7621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 7681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 7741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 7801 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 7861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 7921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 7981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 8221 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 8281 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 8341 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 8401 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 8461 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 8521 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 8581 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 8641 cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.