pCMV-ERBB3-EYFP-Neo plasmid

pCMV-ERBB3-EYFP-Neo plasmid

PVT96150

pCMV-ERBB3-EYFP-Neo plasmid Information

Plasmid name: pCMV-ERBB3(human)-EYFP-Neo
Alias: NM_001982.4;c-erbB-3; c-erbB3; ErbB-3; erbB3-S; FERLK;HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3;VSCN1
Gene lenght: 4026bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ERBB3-EYFP-Neo plasmid Description

pCMV-ERBB3-EYFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ERBB3-EYFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atgagggcga acgacgctct 661 gcaggtgctg ggcttgcttt tcagcctggc ccggggctcc gaggtgggca actctcaggc 721 agtgtgtcct gggactctga atggcctgag tgtgaccggc gatgctgaga accaatacca 781 gacactgtac aagctctacg agaggtgtga ggtggtgatg gggaaccttg agattgtgct 841 cacgggacac aatgccgacc tctccttcct gcagtggatt cgagaagtga caggctatgt 901 cctcgtggcc atgaatgaat tctctactct accattgccc aacctccgcg tggtgcgagg 961 gacccaggtc tacgatggga agtttgccat cttcgtcatg ttgaactata acaccaactc 1021 cagccacgct ctgcgccagc tccgcttgac tcagctcacc gagattctgt cagggggtgt 1081 ttatattgag aagaacgata agctttgtca catggacaca attgactgga gggacatcgt 1141 gagggaccga gatgctgaga tagtggtgaa ggacaatggc agaagctgtc ccccctgtca 1201 tgaggtttgc aaggggcgat gctggggtcc tggatcagaa gactgccaga cattgaccaa 1261 gaccatctgt gctcctcagt gtaatggtca ctgctttggg cccaacccca accagtgctg 1321 ccatgatgag tgtgccgggg gctgctcagg ccctcaggac acagactgct ttgcctgccg 1381 gcacttcaat gacagtggag cctgtgtacc tcgctgtcca cagcctcttg tctacaacaa 1441 gctaactttc cagctggaac ccaatcccca caccaagtat cagtatggag gagtttgtgt 1501 agccagctgt ccccataact ttgtggtgga tcaaacatcc tgtgtcaggg cctgtcctcc 1561 tgacaagatg gaagtagata aaaatgggct caagatgtgt gagccttgtg ggggactatg 1621 tcccaaagcc tgtgagggaa caggctctgg gagccgcttc cagactgtgg actcgagcaa 1681 cattgatgga tttgtgaact gcaccaagat cctgggcaac ctggactttc tgatcaccgg 1741 cctcaatgga gacccctggc acaagatccc tgccctggac ccagagaagc tcaatgtctt 1801 ccggacagta cgggagatca caggttacct gaacatccag tcctggccgc cccacatgca 1861 caacttcagt gttttttcca atttgacaac cattggaggc agaagcctct acaaccgggg 1921 cttctcattg ttgatcatga agaacttgaa tgtcacatct ctgggcttcc gatccctgaa 1981 ggaaattagt gctgggcgta tctatataag tgccaatagg cagctctgct accaccactc 2041 tttgaactgg accaaggtgc ttcgggggcc tacggaagag cgactagaca tcaagcataa 2101 tcggccgcgc agagactgcg tggcagaggg caaagtgtgt gacccactgt gctcctctgg 2161 gggatgctgg ggcccaggcc ctggtcagtg cttgtcctgt cgaaattata gccgaggagg 2221 tgtctgtgtg acccactgca actttctgaa tggggagcct cgagaatttg cccatgaggc 2281 cgaatgcttc tcctgccacc cggaatgcca acccatggag ggcactgcca catgcaatgg 2341 ctcgggctct gatacttgtg ctcaatgtgc ccattttcga gatgggcccc actgtgtgag 2401 cagctgcccc catggagtcc taggtgccaa gggcccaatc tacaagtacc cagatgttca 2461 gaatgaatgt cggccctgcc atgagaactg cacccagggg tgtaaaggac cagagcttca 2521 agactgttta ggacaaacac tggtgctgat cggcaaaacc catctgacaa tggctttgac 2581 agtgatagca ggattggtag tgattttcat gatgctgggc ggcacttttc tctactggcg 2641 tgggcgccgg attcagaata aaagggctat gaggcgatac ttggaacggg gtgagagcat 2701 agagcctctg gaccccagtg agaaggctaa caaagtcttg gccagaatct tcaaagagac 2761 agagctaagg aagcttaaag tgcttggctc gggtgtcttt ggaactgtgc acaaaggagt 2821 gtggatccct gagggtgaat caatcaagat tccagtctgc attaaagtca ttgaggacaa 2881 gagtggacgg cagagttttc aagctgtgac agatcatatg ctggccattg gcagcctgga 2941 ccatgcccac attgtaaggc tgctgggact atgcccaggg tcatctctgc agcttgtcac 3001 tcaatatttg cctctgggtt ctctgctgga tcatgtgaga caacaccggg gggcactggg 3061 gccacagctg ctgctcaact ggggagtaca aattgccaag ggaatgtact accttgagga 3121 acatggtatg gtgcatagaa acctggctgc ccgaaacgtg ctactcaagt cacccagtca 3181 ggttcaggtg gcagattttg gtgtggctga cctgctgcct cctgatgata agcagctgct 3241 atacagtgag gccaagactc caattaagtg gatggccctt gagagtatcc actttgggaa 3301 atacacacac cagagtgatg tctggagcta tggtgtgaca gtttgggagt tgatgacctt 3361 cggggcagag ccctatgcag ggctacgatt ggctgaagta ccagacctgc tagagaaggg 3421 ggagcggttg gcacagcccc agatctgcac aattgatgtc tacatggtga tggtcaagtg 3481 ttggatgatt gatgagaaca ttcgcccaac ctttaaagaa ctagccaatg agttcaccag 3541 gatggcccga gacccaccac ggtatctggt cataaagaga gagagtgggc ctggaatagc 3601 ccctgggcca gagccccatg gtctgacaaa caagaagcta gaggaagtag agctggagcc 3661 agaactagac ctagacctag acttggaagc agaggaggac aacctggcaa ccaccacact 3721 gggctccgcc ctcagcctac cagttggaac acttaatcgg ccacgtggga gccagagcct 3781 tttaagtcca tcatctggat acatgcccat gaaccagggt aatcttgggg agtcttgcca 3841 ggagtctgca gtttctggga gcagtgaacg gtgcccccgt ccagtctctc tacacccaat 3901 gccacgggga tgcctggcat cagagtcatc agaggggcat gtaacaggct ctgaggctga 3961 gctccaggag aaagtgtcaa tgtgtaggag ccggagcagg agccggagcc cacggccacg 4021 cggagatagc gcctaccatt cccagcgcca cagtctgctg actcctgtta ccccactctc 4081 cccacccggg ttagaggaag aggatgtcaa cggttatgtc atgccagata cacacctcaa 4141 aggtactccc tcctcccggg aaggcaccct ttcttcagtg ggtctcagtt ctgtcctggg 4201 tactgaagaa gaagatgaag atgaggagta tgaatacatg aaccggagga gaaggcacag 4261 tccacctcat ccccctaggc caagttccct tgaggagctg ggttatgagt acatggatgt 4321 ggggtcagac ctcagtgcct ctctgggcag cacacagagt tgcccactcc accctgtacc 4381 catcatgccc actgcaggca caactccaga tgaagactat gaatatatga atcggcaacg 4441 agatggaggt ggtcctgggg gtgattatgc agccatgggg gcctgcccag catctgagca 4501 agggtatgaa gagatgagag cttttcaggg gcctggacat caggcccccc atgtccatta 4561 tgcccgccta aaaactctac gtagcttaga ggctacagac tctgcctttg ataaccctga 4621 ttactggcat agcaggcttt tccccaaggc taatgcccag agaacgggtg gaggcggatc 4681 aggaggcggt ggatctgatc caccggtcgc caccatggtg agcaagggcg aggagctgtt 4741 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 4801 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 4861 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct 4921 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 4981 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 5041 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 5101 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 5161 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 5221 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 5281 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag 5341 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 5401 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc 5461 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 5521 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 5581 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 5641 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa 5701 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 5761 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 5821 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 5881 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 5941 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 6001 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 6061 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 6121 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 6181 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 6241 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat 6301 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 6361 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 6421 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 6481 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6541 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6601 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt 6661 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 6721 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 6781 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 6841 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 6901 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 6961 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 7021 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 7081 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 7141 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 7201 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 7261 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 7321 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 7381 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 7441 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 7501 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 7561 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 7621 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 7681 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat 7741 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 7801 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 7861 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat 7921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 7981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 8041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 8101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 8161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 8221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 8281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 8341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 8401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 8461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 8521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 8581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 8641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 8701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 8761 gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.