pLenti-U6-ITGB2-sgRNA1-Cas9-Bl

pLenti-U6-ITGB2-sgRNA1-Cas9-Blast plasmid

PVT96145

pLenti-U6-ITGB2-sgRNA1-Cas9-Blast plasmid Information

Plasmid name: pLenti-U6-ITGB2(human)-sgRNA1-Cas9-Blast
Alias: tcatccccaagtcagccgtg
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLenti-U6-ITGB2-sgRNA1-Cas9-Blast plasmid Description

pLenti-U6-ITGB2-sgRNA1-Cas9-Blast This is a plasmid suitable for gene editing in mammalian cells, and it can also be used for lentivirus packaging.

pLenti-U6-ITGB2-sgRNA1-Cas9-Blast plasmid Sequence

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgtca tccccaagtc agccgtggtt 2881 ttagagctaG AAAtagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc 2941 accgagtcgg tgcTTTTTTg aattcgctag ctaggtcttg aaaggagtgg gaattggctc 3001 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3061 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 3121 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 3181 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag 3241 cgctgccacc ATGGACAAGA AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG 3301 CTGGGCCGTG ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA 3361 CACCGACCGG CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA 3421 AACAGCCGAG GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA 3481 CCGGATCTGC TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT 3541 CTTCCACAGA CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC 3601 CATCTTCGGC AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA 3661 CCTGAGAAAG AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC 3721 CCTGGCCCAC ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA 3781 CAACAGCGAC GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA 3841 GGAAAACCCC ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG 3901 CAAGAGCAGA CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT 3961 GTTCGGAAAC CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA 4021 CCTGGCCGAG GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA 4081 CCTGCTGGCC CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC 4141 CGACGCCATC CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT 4201 GAGCGCCTCT ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC 4261 TCTCGTGCGG CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA 4321 CGGCTACGCC GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA 4381 GCCCATCCTG GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA 4441 CCTGCTGCGG AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG 4501 AGAGCTGCAC GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG 4561 GGAAAAGATC GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG 4621 GGGAAACAGC AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA 4681 CTTCGAGGAA GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA 4741 CTTCGATAAG AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA 4801 CTTCACCGTG TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC 4861 CGCCTTCCTG AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG 4921 GAAAGTGACC GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC 4981 CGTGGAAATC TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT 5041 GCTGAAAATT ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA 5101 AGATATCGTG CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA 5161 AACCTATGCC CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC 5221 CGGCTGGGGC AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA 5281 GACAATCCTG GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT 5341 CCACGACGAC AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG 5401 CGATAGCCTG CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT 5461 CCTGCAGACA GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA 5521 GAACATCGTG ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG 5581 CCGCGAGAGA ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA 5641 AGAACACCCC GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA 5701 GAATGGGCGG GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA 5761 TGTGGACCAT ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT 5821 GACCAGAAGC GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA 5881 GAAGATGAAG AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT 5941 CGACAATCTG ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT 6001 CAAGAGACAG CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC 6061 CCGGATGAAC ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC 6121 CCTGAAGTCC AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA 6181 GATCAACAAC TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT 6241 GATCAAAAAG TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA 6301 CGTGCGGAAG ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT 6361 CTTCTACAGC AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT 6421 CCGGAAGCGG CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG 6481 CCGGGATTTT GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA 6541 GACCGAGGTG CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA 6601 TAAGCTGATC GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC 6661 CACCGTGGCC TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT 6721 GAAGAGTGTG AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA 6781 TCCCATCGAC TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA 6841 GCTGCCTAAG TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC 6901 CGGCGAACTG CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA 6961 CCTGGCCAGC CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT 7021 GTTTGTGGAA CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC 7081 CAAGAGAGTG ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA 7141 CCGGGATAAG CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA 7201 TCTGGGAGCC CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC 7261 CAGCACCAAA GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA 7321 GACACGGATC GACCTGTCTC AGCTGGGAGG CGACAAGCGA CCTGCCGCCA CAAAGAAGGC 7381 TGGACAGGCT AAGAAGAAGA AAGATTACAA AGACGATGAC GATAAGGGAT CCGGCGCAAC 7441 AAACTTCTCT CTGCTGAAAC AAGCCGGAGA TGTCGAAGAG AATCCTGGAC CGATGGCCAA 7501 GCCTTTGTCT CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT 7561 CCCCATCTCT GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT 7621 CACTGGTGTC AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG 7681 CACTGCTGCT GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA 7741 CAGGGGCATC TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG 7801 GATCAAAGCC ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT 7861 GCTGCCCTCT GGTTATGTGT GGGAGGGCTA AGAATTCGAT ATCAAGCTTA TCGGTAATCA 7921 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 7981 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 8041 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 8101 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 8161 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 8221 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 8281 CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG 8341 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 8401 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT 8461 TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCGTCGAC TTTAAGACCA 8521 ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA 8581 GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG 8641 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT 8701 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT 8761 AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG ggcccgttta 8821 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 8881 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 8941 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 9001 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 9061 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 9121 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 9181 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 9241 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 9301 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 9361 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 9421 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 9481 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 9541 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 9601 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 9661 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 9721 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 9781 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 9841 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 9901 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg 9961 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc 10021 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 10081 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 10141 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 10201 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 10261 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 10321 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac 10381 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 10441 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 10501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 10561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 10621 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 10681 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 10741 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 10801 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 10861 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 10921 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 10981 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 11041 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 11101 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 11161 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 11221 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 11281 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 11341 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 11401 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 11461 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 11521 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 11581 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 11641 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 11701 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 11761 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 11821 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 11881 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 11941 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 12001 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 12061 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 12121 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 12181 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 12241 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 12301 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 12361 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 12421 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 12481 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 12541 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 12601 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 12661 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 12721 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 12781 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.