pLV2-CMV-AGR3-3×FLAG-Puro plas

pLV2-CMV-AGR3-3×FLAG-Puro plasmid

PVT96133

pLV2-CMV-AGR3-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-AGR3(human)-3×FLAG-Puro
Alias: NM_176813.5;AG-3; AG3; BCMP11; hAG-3; HAG3; PDIA18
Gene lenght: 498bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-AGR3-3×FLAG-Puro plasmid Description

pLV2-CMV-AGR3-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-AGR3-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgatgct acactcagct ttgggtctct gcctcttact 2881 cgtcacagtt tcttccaacc ttgccattgc aataaaaaag gaaaagaggc ctcctcagac 2941 actctcaaga ggatggggag atgacatcac ttgggtacaa acttatgaag aaggtctctt 3001 ttatgctcaa aaaagtaaga agccattaat ggttattcat cacctggagg attgtcaata 3061 ctctcaagca ctaaagaaag tatttgccca aaatgaagaa atacaagaaa tggctcagaa 3121 taagttcatc atgctaaacc ttatgcatga aaccactgat aagaatttat cacctgatgg 3181 gcaatatgtg cctagaatca tgtttgtaga cccttcttta acagttagag ctgacatagc 3241 tggaagatac tctaacagat tgtacacata tgagcctcgg gatttacccc tattgataga 3301 aaacatgaag aaagcattaa gacttattca gtcagagcta ggatccGACT ACAAAGACCA 3361 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagtctag 3421 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 3481 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 3541 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 3601 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 3661 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 3721 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 3781 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 3841 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 3901 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 3961 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4021 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4081 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4141 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4201 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4261 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4321 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4381 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4441 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4501 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 4561 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4621 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4681 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4741 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4801 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 4861 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 4921 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 4981 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5041 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5101 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 5161 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5221 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 5281 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5341 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 5401 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 5461 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 5521 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 5581 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 5641 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 5701 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 5761 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 5821 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 5881 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5941 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6001 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6061 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 6121 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 6181 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 6241 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 6301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 6361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 6421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 6481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 6541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 6601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 6661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 6721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 6781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 7201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 7261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 7321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 7381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 7441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 7501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 7561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 7621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 7741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 7801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 7861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 7921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 7981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 8041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 8161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 8221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 8281 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 8341 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 8401 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 8461 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 8521 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 8581 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag 8641 tagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.