pLV2-CMV-Eif2ak2-EGFP plasmid

pLV2-CMV-Eif2ak2-EGFP plasmid

PVT96122

pLV2-CMV-Eif2ak2-EGFP plasmid Information

Plasmid name: pLV2-CMV-Eif2ak2(mouse)-EGFP
Alias: NM_011163.4;2310047A08Rik; 4732414G15Rik; Pkr; Prkr;Tik
Gene lenght: 1545bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Eif2ak2-EGFP plasmid Description

pLV2-CMV-Eif2ak2-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-Eif2ak2-EGFP plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 2821 tctagagcca ccatggccag tgatacccca ggtttctaca tggacaaact taataaatac 2881 cgccagatgc acggagtagc cattacgtat aaagaactta gtacttcggg acctccacat 2941 gacagaaggt ttacatttca agttttaata gatgagaagg aatttccaga agccaaaggt 3001 agatcaaagc aggaggcaag aaacgctgca gccaaattag ctgttgatat acttgataac 3061 gaaaacaagg tggattgtca cacgagtgca tctgagcaag gcttgttcgt tggtaactac 3121 ataggccttg tcaatagctt tgcccagaag aaaaagctgt ctgtaaatta tgaacagtgt 3181 gagcccaact ctgagttgcc tcaaagattt atttgtaaat gcaaaattgg gcagacaatg 3241 tatggtactg gttcaggtgt caccaaacag gaggcaaagc agttggctgc gaaagaagcc 3301 tatcagaagc tgttaaagag cccgccgaaa actgccggaa catcctctag cgttgtcaca 3361 tctacattca gtggcttttc cagcagctcg tctatgacaa gtaatggtgt ttcccagtca 3421 gcacctggaa gtttttcctc agagaacgtg tttacgaacg gtctcggaga aaataaaagg 3481 aaatcaggag taaaagtatc ccctgatgat gtgcaaagaa ataaatatac cttggacgcc 3541 aggtttaaca gcgattttga agacatagaa gaaattggct taggtggatt tggtcaagtt 3601 ttcaaagcga aacacagaat tgatggaaag agatacgcta ttaagcgcgt taaatataac 3661 acggagaagg cggagcacga agtacaagcg ctggcagaac tcaatcacgt caacattgtc 3721 caataccata gttgttggga gggagttgac tatgatcctg agcacagcat gagtgataca 3781 agtcgataca aaacccggtg cctctttatt caaatggaat tctgtgataa aggaactttg 3841 gagcaatgga tgagaaacag aaatcagagt aaagtggaca aagctttgat tttggactta 3901 tatgaacaaa tcgtgaccgg agtggagtat atacactcga aagggttaat tcacagagat 3961 cttaagccag gtaatatatt tttagtagat gaaagacaca ttaagatcgg agactttggc 4021 cttgcaacag ccctggaaaa tgatggaaaa tcccgaacaa ggagaacagg aactcttcaa 4081 tacatgagtc cagaacagtt atttttaaag cactatggaa aagaagtgga catctttgct 4141 ttgggcctta ttctagctga acttcttcac acgtgcttca cggagtcaga gaaaataaag 4201 tttttcgaaa gtctaagaaa aggcgacttc tctaatgata tattcgacaa caaagaaaaa 4261 agccttctaa aaaaactact ctcagagaaa cccaaggacc gacctgagac atctgaaatc 4321 ctgaagacct tggctgaatg gaggaacatc tcagagaaaa agaaaagaaa cacatgtgga 4381 tccgcggccg ctgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 4441 ggccctaccg gtatcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 4501 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 4561 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 4621 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac 4681 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 4741 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 4801 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 4861 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 4921 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 4981 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 5041 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 5101 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 5161 gagctgtaca agtaaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag 5221 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 5281 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 5341 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 5401 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 5461 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 5521 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 5581 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 5641 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 5701 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 5761 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca 5821 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 5881 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 5941 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac 6001 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 6061 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 6121 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 6181 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 6241 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 6301 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 6361 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 6421 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 6481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 6541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 6601 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 6661 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc 6721 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 6781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 6841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 6901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 6961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 7441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 7621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 7861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 7921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 8401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 8821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 8881 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata 8941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 9001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 9061 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 9121 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 9181 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg 9241 ttaaatatgt actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.