pLV3-CMV-ROBO1-3×HA-Puro plasm

pLV3-CMV-ROBO1-3×HA-Puro plasmid

PVT95954

pLV3-CMV-ROBO1-3×HA-Puro plasmid Information

Plasmid name: pLV3-CMV-ROBO1(human)-3×HA-Puro
Alias: NM_002941.4 ;CPHD8; DUTT1; NORS; NYS8; SAX3
Gene lenght: 4953bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ROBO1-3×HA-Puro plasmid Description

pLV3-CMV-ROBO1-3×HA-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ROBO1-3×HA-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgaaatgg aaacatgttc cttttttggt catgatatca 2341 ctcctcagct tatccccaaa tcacctgttt ctggcccagc ttattccaga ccctgaagat 2401 gtagagaggg ggaacgacca cgggacgcca atccccacct ctgataacga tgacaattcg 2461 ctgggctata caggctcccg tcttcgtcag gaagattttc cacctcgcat tgttgaacac 2521 ccttcagacc tgattgtctc aaaaggagaa cctgcaactt tgaactgcaa agctgaaggc 2581 cgccccacac ccactattga atggtacaaa gggggagaga gagtggagac agacaaagat 2641 gaccctcgct cacaccgaat gttgctgccg agtggatctt tatttttctt acgtatagta 2701 catggacgga aaagtagacc tgatgaagga gtctatgtct gtgtagcaag gaattacctt 2761 ggagaggctg tgagccacaa tgcatcgctg gaagtagcca tacttcggga tgacttcaga 2821 caaaaccctt cggatgtcat ggttgcagta ggagagcctg cagtaatgga atgccaacct 2881 ccacgaggcc atcctgagcc caccatttca tggaagaaag atggctctcc actggatgat 2941 aaagatgaaa gaataactat acgaggagga aagctcatga tcacttacac ccgtaaaagt 3001 gacgctggca aatatgtttg tgttggtacc aatatggttg gggaacgtga gagtgaagta 3061 gccgagctga ctgtcttaga gagaccatca tttgtgaaga gacccagtaa cttggcagta 3121 actgtggatg acagtgcaga atttaaatgt gaggcccgag gtgaccctgt acctacagta 3181 cgatggagga aagatgatgg agagctgccc aaatccagat atgaaatccg agatgatcat 3241 accttgaaaa ttaggaaggt gacagctggt gacatgggtt catacacttg tgttgcagaa 3301 aatatggtgg gcaaagctga agcatctgct actctgactg ttcaagaacc tccacatttt 3361 gttgtgaaac cccgtgacca ggttgttgct ttgggacgga ctgtaacttt tcagtgtgaa 3421 gcaaccggaa atcctcaacc agctattttc tggaggagag aagggagtca gaatctactt 3481 ttctcatatc aaccaccaca gtcatccagc cgattttcag tctcccagac tggcgacctc 3541 acaattacta atgtccagcg atctgatgtt ggttattaca tctgccagac tttaaatgtt 3601 gctggaagca tcatcacaaa ggcatatttg gaagttacag atgtgattgc agatcggcct 3661 cccccagtta ttcgacaagg tcctgtgaat cagactgtag ccgtggatgg cactttcgtc 3721 ctcagctgtg tggccacagg cagtccagtg cccaccattc tgtggagaaa ggatggagtc 3781 ctcgtttcaa cccaagactc tcgaatcaaa cagttggaga atggagtact gcagatccga 3841 tatgctaagc tgggtgatac tggtcggtac acctgcattg catcaacccc cagtggtgaa 3901 gcaacatgga gtgcttacat tgaagttcaa gaatttggag ttccagttca gcctccaaga 3961 cctactgacc caaatttaat ccctagtgcc ccatcaaaac ctgaagtgac agatgtcagc 4021 agaaatacag tcacattatc gtggcaacca aatttgaatt caggagcaac tccaacatct 4081 tatattatag aagccttcag ccatgcatct ggtagcagct ggcagaccgt agcagagaat 4141 gtgaaaacag aaacatctgc cattaaagga ctcaaaccta atgcaattta ccttttcctt 4201 gtgagggcag ctaatgcata tggaattagt gatccaagcc aaatatcaga tccagtgaaa 4261 acacaagatg tcctaccaac aagtcagggg gtggaccaca agcaggtcca gagagagctg 4321 ggaaatgctg ttctgcacct ccacaacccc accgtccttt cttcctcttc catcgaagtg 4381 cactggacag tagatcaaca gtctcagtat atacaaggat ataaaattct ctatcggcca 4441 tctggagcca accacggaga atcagactgg ttagtttttg aagtgaggac gccagccaaa 4501 aacagtgtgg taatccctga tctcagaaag ggagtcaact atgaaattaa ggctcgccct 4561 ttttttaatg aatttcaagg agcagatagt gaaatcaagt ttgccaaaac cctggaagaa 4621 gcacccagtg ccccacccca aggtgtaact gtatccaaga atgatggaaa cggaactgca 4681 attctagtta gttggcagcc acctccagaa gacactcaaa atggaatggt ccaagagtat 4741 aaggtttggt gtctgggcaa tgaaactcga taccacatca acaaaacagt ggatggttcc 4801 accttttccg tggtcattcc ctttcttgtt cctggaatcc gatacagtgt ggaagtggca 4861 gccagcactg gggctgggtc tggggtaaag agtgagcctc agttcatcca gctggatgcc 4921 catggaaacc ctgtgtcacc tgaggaccaa gtcagcctcg ctcagcagat ttcagatgtg 4981 gtgaagcagc cggccttcat agcaggtatt ggagcagcct gttggatcat cctcatggtc 5041 ttcagcatct ggctttatcg acaccgcaag aagagaaacg gacttactag tacctacgcg 5101 ggtatcagaa aagtcccgtc ttttaccttc acaccaacag taacttacca gagaggaggc 5161 gaagctgtca gcagtggagg gaggcctgga cttctcaaca tcagtgaacc tgccgcgcag 5221 ccatggctgg cagacacgtg gcctaatact ggcaacaacc acaatgactg ctccatcagc 5281 tgctgcacgg caggcaatgg aaacagcgac agcaacctca ctacctacag tcgcccagct 5341 gattgtatag caaattataa caaccaactg gataacaaac aaacaaatct gatgctccct 5401 gagtcaactg tttatggtga tgtggacctt agtaacaaaa tcaatgagat gaaaaccttc 5461 aatagcccaa atctgaagga tgggcgtttt gtcaatccat cagggcagcc tactccttac 5521 gccaccactc agctcatcca gtcaaacctc agcaacaaca tgaacaatgg cagcggggac 5581 tctggcgaga agcactggaa accactggga cagcagaaac aagaagtggc accagttcag 5641 tacaacatcg tggagcaaaa caagctgaac aaagattatc gagcaaatga cacagttcct 5701 ccaactatcc catacaacca atcatacgac cagaacacag gaggatccta caacagctca 5761 gaccggggca gtagtacatc tgggagtcag gggcacaaga aaggggcaag aacacccaag 5821 gtaccaaaac agggtggcat gaactgggca gacctgcttc ctcctccccc agcacatcct 5881 cctccacaca gcaatagcga agagtacaac atttctgtag atgaaagcta tgaccaagaa 5941 atgccatgtc ccgtgccacc agcaaggatg tatttgcaac aagatgaatt agaagaggag 6001 gaagatgaac gaggccccac tccccctgtt cggggagcag cttcttctcc agctgccgtg 6061 tcctatagcc atcagtccac tgccactctg actccctccc cacaggaaga actccagccc 6121 atgttacagg attgtccaga ggagactggc cacatgcagc accagcccga caggagacgg 6181 cagcctgtga gtcctcctcc accaccacgg ccgatctccc ctccacatac ctatggctac 6241 atttcaggac ccctggtctc agatatggat acggatgcgc cagaagagga agaagacgaa 6301 gccgacatgg aggtagccaa gatgcaaacc agaaggcttt tgttacgtgg gcttgagcag 6361 acacctgcct ccagtgttgg ggacctggag agctctgtca cggggtccat gatcaacggc 6421 tggggctcag cctcagagga ggacaacatt tccagcggac gctccagtgt tagttcttcg 6481 gacggctcct ttttcactga tgctgacttt gcccaggcag tcgcagcagc ggcagagtat 6541 gctggtctga aagtagcacg acggcaaatg caggatgctg ctggccgtcg acattttcat 6601 gcgtctcagt gccctaggcc cacaagtccc gtgtctacag acagcaacat gagtgccgcc 6661 gtaatgcaga aaaccagacc agccaagaaa ctgaaacacc agccaggaca tctgcgcaga 6721 gaaacctaca cagatgatct tccaccacct cctgtgccgc cacctgctat aaagtcacct 6781 actgcccaat ccaagacaca gctggaagta cgacctgtag tggtgccaaa actcccttct 6841 atggatgcaa gaacagacag atcatcagac agaaaaggaa gcagttacaa ggggagagaa 6901 gtgttggatg gaagacaggt tgttgacatg cgaacaaatc caggtgatcc cagagaagca 6961 caggaacagc aaaatgacgg gaaaggacgt ggaaacaagg cagcaaaacg agaccttcca 7021 ccagcaaaga ctcatctcat ccaagaggat attctacctt attgtagacc tacttttcca 7081 acatcaaata atcccagaga tcccagttcc tcaagctcaa tgtcatcaag aggatcagga 7141 agcagacaaa gagaacaagc aaatgtaggt cgaagaaata ttgcagaaat gcaggtactt 7201 ggaggatatg aaagaggaga agataataat gaagaattag aggaaactga aagcGGATCC 7261 TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC 7321 TATCCTTATG ATGTCCCAGA CTACGCATAG GCGGCCGCGA AGGATCTGCG ATCGCTCCGG 7381 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 7441 CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 7501 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 7561 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC 7621 TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG 7681 TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG 7741 TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT 7801 CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT 7861 TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ATGACCGAGT 7921 ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG 7981 CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG 8041 AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG 8101 TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG 8161 CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG 8221 CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT 8281 TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG 8341 TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG 8401 CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG 8461 TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTCGACAATC 8521 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT 8581 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG 8641 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC 8701 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT 8761 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG 8821 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG 8881 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT 8941 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC 9001 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC 9061 TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT ACCTTTAAGA 9121 CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG 9181 GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC 9241 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG 9301 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT 9361 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT 9421 TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG 9481 AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 9541 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 9601 TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 9661 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG 9721 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC 9781 CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 9841 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 9901 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 9961 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 10021 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 10081 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 10141 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 10201 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 10261 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 10321 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 10381 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 10441 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 10501 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 10561 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 10621 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 10681 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 10741 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 10801 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 10861 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 10921 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 10981 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 11041 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 11101 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 11161 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 11221 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 11281 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 11341 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 11401 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 11461 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 11521 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 11581 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 11641 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 11701 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 11761 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 11821 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 11881 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 11941 TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA 12001 TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 12061 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 12121 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 12181 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 12241 AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG 12301 TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA 12361 GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC 12421 TG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.