pCMV-EGFP-Mrc1-Neo plasmid

pCMV-EGFP-Mrc1-Neo plasmid

PVT95935

pCMV-EGFP-Mrc1-Neo plasmid Information

Plasmid name: pCMV-EGFP-Mrc1(mouse-toxic-opt)-Neo
Alias: NM_008625.2;CD206; MR;
Gene lenght: 4368bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): EPI300
Growth Temperature: 30°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: Low
Induction:

pCMV-EGFP-Mrc1-Neo plasmid Description

pCMV-EGFP-Mrc1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Mrc1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT Catgaggctt ctcctgcttc tggcttttat ctctgtcatc 1681 cctgtctctg ttcagctatt ggacgcgagg caatttttaa tctataatga agatcacaag 1741 cgctgcgtgg acgctctaag tgccatctca gttcagacgg caacttgcaa cccggaagct 1801 gaatcccaga aattccgctg ggtgtcagat tctcagatca tgagtgttgc tttcaaatta 1861 tgtttgggag tgccatcaaa aactgactgg gcttccgtca ccctgtatgc ctgtgattcg 1921 aaaagtgaat atcagaaatg ggagtgtaag aatgacacac tctttggaat caagggcaca 1981 gagttatatt ttaattatgg caacagacaa gagaagaata tcaagcttta caaaggttcg 2041 ggattgtgga gcagatggaa ggtctatgga accacggatg acctgtgctc gagaggatat 2101 gaagccatgt actccttact gggcaatgca aatggagccg tctgtgcatt tccattcaag 2161 tttgaaaaca agtggtatgc agactgcacc tctgccgggc gctcggacgg atggctctgg 2221 tgtggaacca ccactgacta cgacaaagac aagctgtttg gattttgtcc attgcacttt 2281 gagggaagcg agagattatg gaacaaagat ccactgactg gcattcttta ccagataaac 2341 tccaagtctg ctttaacctg gcatcaggca agggcaagct gcaagcagca gaatgctgac 2401 ctcctgagtg tcacggagat ccacgagcaa ATGTATCTGA CTGGGCTGAC TTCTAGCCTT 2461 AGCTCTGGTC TGTGGATCGG CCTGAACTCC CTGTCTGTCC GAAGCGGCTG GCAGTGGGCC 2521 GGCGGCTCTC CTTTCAGGTA CTTGAACTGG CTGCCTGGGA GCCCCTCTAG CGAGCCCGGC 2581 AAGTCCTGCG TCAGCCTGAA CCCAGGAAAA AACGCCAAAT GGGAGAACCT GGAATGCGTG 2641 CAGAAGCTGG GCTATATCTG CAAAAAGGGC AACAACACCC TGAACCCATT CATCATTCCC 2701 TCCGCTTCAG ACGTGCCTAC CGGCTGCCCA AATCAGTGGT GGCCCTACGC CGGCCACTGT 2761 TACAGAATCC ACAGGGAGGA AAAGAAGATC CAGAAGTATG CCCTGCAGGC CTGTCGGAAG 2821 GAGGGTGGAG ATCTGGCCAG TATCCACTCT ATCGAGGAGT TCGACTTTAT CTTTTCTCAG 2881 CTGGGCTACG AGCCCAACGA TGAGCTGTGG ATCGGCCTGA ATGATATCAA AATCCAGATG 2941 TACTTCGAAT GGAGCGACGG CACCCCCGTG ACCTTCACCA AGTGGCTGCC CGGCGAGCCC 3001 AGCCACGAGA ACAACAGACA GGAGGACTGC GTGGTGATGA AGGGAAAGGA TGGCTATTGG 3061 GCAGACCGAG CCTGTGAGCA GCCTCTGGGC TATATTTGTA AGATGGTGTC TCAGTCCCAC 3121 GCCGTGGTGC CCGAGGGCGC TGACAAGGGG TGTAGAAAGG GCTGGAAGAG GCACGGCTTC 3181 TATTGCTACC TCATCGGCTC CACCCTGAGC ACCTTCACCG ACGCCAACCA TACATGCACC 3241 AATGAGAAGG CTTACCTGAC TACAGTGGAG GACAGGTATG AGCAGGCCTT TCTCACATCT 3301 CTGGTGGGAC TGAGGCCCGA GAAGTACTTT TGGACCGGAC TGAGCGACGT GCAGAACAAG 3361 GGAACCTTCA GGTGGACAGT GGACGAGCAG GTGCAGTTTA CCCACTGGAA CGCCGACATG 3421 CCAGGCAGGA AGGCCGGCTG TGTGGCCATG AAGACCGGAG TGGCCGGAGG ACTCTGGGAC 3481 GTGCTGAGCT GCGAGGAGAA GGCCAAGTTT GTGTGTAAGC ACTGGGCCGA GGGCGTGACC 3541 AGACCTCCCG AACCCACAAC CACACCCGAA CCCAAGTGTC CAGAGAACTG Gggtaccacc 3601 agtaaaacca gcatgtgttt caaactgtat gcaaaaggaa agcatgaaaa gaaaacgtgg 3661 tttgaatctc gagatttttg caaagctata ggtggagagc tggcgagcat caagagtaaa 3721 gatgaacagc aagtgatttg gaggctgatt acgagcagtg gaagctacca tgagctgttt 3781 tggttgggac tgacctatgg aagtccttca gaggggttca cctggagtga tggttctccc 3841 gtttcctatg aaaattgggc ttacggtgaa ccaaataatt accaaaatgt tgaatattgt 3901 ggtgagctga aaggtgaccc tggcatgtcc tggaatgata tcaactgtga acacctcaac 3961 aactggattt gtcagataca aaaagggaaa acactactac ctgagcccac acctgctcca 4021 caagacaatc caccagttac tgcagatggg tgggttattt acaaagacta ccagtactat 4081 tttagcaaag agaaggaaac catggacaac gcgcgagcat tttgcaagaa gaattttggt 4141 gatcttgcta caattaaaag tgaaagtgaa aagaagtttc tatggaaata tataaacaag 4201 aatggtgggc agtcaccata ttttattggc atgttaatca gcatggataa gaaattcatt 4261 tggatggatg ggagcaaagt agattttgtg gcttgggcta caggagaacc caactttgca 4321 aatgatgatg aaaactgtgt aacgatgtac acaaattcag ggttctggaa tgacatcaac 4381 tgtggttatc caaataactt catctgccag agacataaca gcagcatcaa tgccactgcc 4441 atgcctacca cacccacgac accaggtggc tgcaaggaag gttggcattt gtacaagaac 4501 aagtgcttta aaatttttgg atttgctaat gaagaaaaaa aaagctggca agacgcacgg 4561 caagcttgca aaggactgaa aggaaacctg gtgtccatag aaaatgcaca agagcaagca 4621 tttgttacct atcacatgag agactccact ttcaatgcct ggactgggct gaatgatatc 4681 aacgcagaac acatgttcct gtggacagct ggacaaggag ttcattatac aaactggggg 4741 aaaggctatc ctggtggaag aagaagtagc ctatcttatg aagatgctga ctgtgtagtt 4801 gtgattggtg gcaattcacg agaggcaggg acctggatgg atgacacctg tgacagtaaa 4861 caaggctata tatgtcaaac acagactgac ccttccctgc ctgtttctcc aaccactact 4921 ccaaaagatg gctttgttac atatgggaaa agcagctatt cccttatgaa attgaagcta 4981 ccatggcatg aagcagagac atattgcaag gatcatactt ccctgcttgc tagcattcta 5041 gacccctaca gtaatgcatt tgcatggatg aaaatgcacc catttaatgt acccatatgg 5101 attgccctga acagcaactt gaccaataat gaatatactt ggacggatag atggagggtg 5161 cggtacacta actggggtgc tgacgagccg aagcttaagt cagcatgtgt ttacatggat 5221 gttgatggct actggagaac atcatactgc aatgaaagtt tttattttct ctgcaaaaaa 5281 tcagacgaaa tccctgctac tgaacctcct caactgcctg gcaagtgtcc agagtcagaa 5341 cagactgcgt ggattccttt ctatggccat tgctactatt ttgaatcttc ttttacgaga 5401 agttggggtc aggcttctct ggaatgcctt cgaatgggtg cctccctggt ttccatcgag 5461 actgctgctg agtccagttt tctgtcatac cgtgttgaac ctcttaaaag taaaaccaat 5521 ttttggatag gcatgttccg aaatgttgaa gggaagtggc tttggttgaa cgacaatcct 5581 gtctcctttg tcaactggaa aacaggcgat ccctctggtg aacggaatga ttgtgtagtt 5641 ctagcttcat cttcgggcct ttggaataat atccactgtt cttcgtacaa aggatttatt 5701 tgtaaaatgc caaaaattat tgatcctgta actacacact catccattac aaccaaagct 5761 gaccaaagga agatggatcc tcaacccaag ggctcttcta aagcagcagg agtggtcacc 5821 gtggtcctcc tgattgtgat aggtgccggc gttgcagcct atttctttta taagaaaagg 5881 catgcgttgc acatacctca agaggccacc tttgaaaaca ctctctactt caacagtaat 5941 ctgagtccag gaacaagtga cacgaaagat ctcatgggca acatcgagca gaatgagcat 6001 gcgatcattt aGGATCCtGA TATCCAGCAC AGTGGCGGCC GCTCGAGTCT AGAGGGCCCG 6061 TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC 6121 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA 6181 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG 6241 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG 6301 GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC 6361 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 6421 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 6481 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 6541 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 6601 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 6661 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 6721 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 6781 ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 6841 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG 6901 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 6961 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 7021 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT 7081 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC 7141 TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA 7201 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 7261 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 7321 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA 7381 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT 7441 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 7501 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT 7561 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 7621 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA 7681 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA 7741 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 7801 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT 7861 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 7921 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT 7981 CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA 8041 CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG 8101 GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC 8161 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA 8221 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 8281 TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG 8341 TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA 8401 AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA 8461 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 8521 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 8581 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 8641 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 8701 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 8761 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 8821 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 8881 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 8941 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 9001 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 9061 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 9121 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 9181 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 9241 TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 9301 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 9361 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 9421 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 9481 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 9541 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 9601 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA 9661 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 9721 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 9781 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 9841 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 9901 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 9961 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 10021 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 10081 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 10141 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 10201 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 10261 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 10321 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 10381 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 10441 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT 10501 CTCCCGATCC CCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.