pCMV-Il33-EGFP-Neo plasmid

pCMV-Il33-EGFP-Neo plasmid

PVT95901

pCMV-Il33-EGFP-Neo plasmid Information

Plasmid name: pCMV-Il33(mouse)-EGFP-Neo
Alias: NM_001164724.2;9230117N10Rik; Il-33; Il1f11; NF-HEV
Gene lenght: 798bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pCMV-Il33-EGFP-Neo plasmid Description

pCMV-Il33-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Il33-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatga gacctagaat gaagtattcc aactccaaga 961 tttccccggc aaagttcagc agcaccgcag gcgaagccct ggtcccgcct tgcaaaataa 1021 gaagatccca acagaagacc aaagaattct gccatgtcta ctgcatgaga ctccgttctg 1081 gcctcaccat aagaaaggag actagttatt ttaggaaaga acccacgaaa agatattcac 1141 taaaatcggg taccaagcat gaagagaact tctctgccta tccacgggat tctaggaaga 1201 gatccttgct tggcagtatc caagcatttg ctgcgtctgt tgacacattg agcatccaag 1261 gaacttcact tttaacacag tctcctgcct ccctgagtac atacaatgac caatctgtta 1321 gttttgtttt ggagaatgga tgttatgtga tcaatgttga cgactctgga aaagaccaag 1381 agcaagacca ggtgctacta cgctactatg agtctccctg tcctgcaagt caatcaggcg 1441 acggtgtgga tgggaagaag ctgatggtga acatgagtcc catcaaagac acagacatct 1501 ggctgcatgc caacgacaag gactactccg tggagcttca aaggggtgac gtctcgcctc 1561 cggaacaggc cttcttcgtc cttcacaaaa agtcctcgga ctttgtttca tttgaatgca 1621 agaatcttcc tggcacttac ataggagtaa aagataacca gctggctcta gtggaggaga 1681 aagatgagag ctgcaacaat attatgttta agctctcgaa aatcggcggt ggaggaagcg 1741 gcggaggtgg atctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 1801 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 1861 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 1921 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 1981 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 2041 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 2101 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 2161 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 2221 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 2281 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 2341 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 2401 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 2461 agctgtacaa gtagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt 2521 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 2581 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 2641 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 2701 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 2761 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 2821 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 2881 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 2941 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 3001 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 3061 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 3121 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 3181 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 3241 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3301 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3361 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 3421 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 3481 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 3541 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 3601 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3661 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 3721 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 3781 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 3841 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 3901 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 3961 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4021 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4081 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 4141 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 4201 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 4261 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 4321 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 4381 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4441 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4501 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4561 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 4621 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 4681 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 4741 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 4801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 4861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 4921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 4981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 5341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 5401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 5461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 5521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 5581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 5641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt 5701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 5821 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 5881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 5941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 6001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 6061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 6121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 6181 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 6241 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 6301 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 6361 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 6421 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 6481 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 6541 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 6601 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 6661 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 6721 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 6781 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 6841 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 6901 ccacctgacg tc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.