pLV3-CMV-KIF13B-3×FLAG-CopGFP-

pLV3-CMV-KIF13B-3×FLAG-CopGFP-Puro plasmid

PVT91751

pLV3-CMV-KIF13B-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KIF13B(human)-3×FLAG-CopGFP-Puro
Alias: NM_015254.4;GAKIN;
Gene lenght: 5478bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KIF13B-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-KIF13B-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KIF13B-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gggactccaa agtgaaagtg 2341 gcggtgcgga tacgacccat gaaccggcga gagactgact tgcataccaa atgtgtggtg 2401 gatgtggatg caaacaaggt tattcttaat cctgtaaata cgaatctttc caaaggagat 2461 gcccggggcc agccgaaggt gtttgcttat gatcattgtt tctggtctat ggatgaatct 2521 gtcaaagaaa agtatgcagg tcaagatatt gttttcaagt gccttggaga gaatatcctg 2581 cagaatgctt ttgatggcta caatgcatgt atctttgcct atggacagac tggctctgga 2641 aaatcttata ccatgatggg cacagctgac caacctggat taatcccaag actttgcagt 2701 ggactctttg aacgaactca gaaagaggaa aatgaagaac agagttttaa agtagaagtg 2761 tcctacatgg aaatttataa tgaaaaagtt cgagaccttc ttgatcccaa aggaagccgt 2821 cagacgttga aagtcagaga gcatagtgtg ttgggacctt atgtcgacgg actttctaaa 2881 ctggctgtca caagctacaa ggatattgag tcgttgatgt ctgagggtaa caaatctcgc 2941 acagttgctg caaccaacat gaacgaggag agtagccgat cccatgcagt tttcaaaatc 3001 accctcacac atactctcta cgatgtgaag tctgggacat ctggagagaa agtgggcaaa 3061 ctcagcctgg tggatttagc tggcagtgaa cgagcaacga agacaggcgc tgcaggggac 3121 aggctgaagg aagggagcaa cattaacaag tccctcacaa ccctcggtct ggttatctca 3181 gctcttgcag atcagagtgc tggcaaaaac aagaataaat ttgttccata tcgtgactca 3241 gttctcactt ggctgctcaa agacagcctc gggggtaaca gcaagaccgc catggtggct 3301 actgtgagtc ctgcagctga taactatgat gaaaccctct caactctgcg gtatgcagat 3361 cgagccaagc acattgtaaa ccacgctgtg gtgaatgagg accctaatgc ccgaattatc 3421 cgggatctcc gggaagaagt tgagaaactc cgggagcagc tgaccaaagc agaggcaatg 3481 aaatctccag agctaaagga ccggctggaa gaatctgaga agctaatcca ggaaatgact 3541 gtgacctggg aggagaaatt aaggaaaacg gaggagattg cacaggaacg acagaaacag 3601 cttgagagtc ttggaatatc tcttcagtct tcgggaatca aagttgggga tgataaatgc 3661 ttccttgtga atctgaatgc tgacccagct ctgaatgagc ttctggtgta ctatttaaag 3721 gaacatacat tgatagggtc agcaaattcc caagatatcc aactgtgcgg catgggaatt 3781 cttcctgaac actgtattat agacatcacg tcagaaggcc aggttatgct gactcctcag 3841 aagaacacca gaacatttgt aaatgggtca tctgtctcca gtccaataca gctacaccat 3901 ggggacagga tattatgggg aaacaatcat ttcttcagac tcaatttgcc taaaaagaaa 3961 aagaaagcag aacgagagga tgaggaccag gatccctcca tgaagaacga gaatagttct 4021 gagcagctgg atgtagacgg agactcctcc agcgaggtgt ccagtgaagt taactttaat 4081 tacgaatacg cacagatgga ggtcaccatg aaggccctgg gcagcaatga tccgatgcag 4141 tccatattaa acagcttaga acaacagcat gaagaagaaa aacgatctgc actggagcgc 4201 cagaggctta tgtatgagca cgaattggag cagctccgga gaaggctgtc tcctgagaag 4261 cagaactgcc ggagcatgga caggttttct ttccactcgc ccagcgctca gcaacgctta 4321 agacagtggg ctgaggagag agaagcaacg ttgaataaca gcctgatgag gctgagggaa 4381 caaattgtta aggccaatct attggtgaga gaagctaatt acattgctga ggagctggat 4441 aaaagaacag aatacaaagt taccctacag attccagcct ccagcctgga tgccaacagg 4501 aagcgaggct ctcttcttag tgagcctgca atccaggtga gaagaaaagg aaaaggaaag 4561 cagatttggt ctttggaaaa actggacaac aggctgttgg atatgagaga cctttatcag 4621 gagtggaaag agtgtgaaga agataaccca gtaatacgat catacttcaa acgtgctgat 4681 ccattctatg atgagcagga aaatcacagt ctcattgggg tggccaatgt cttcctcgag 4741 tcacttttct atgatgtgaa gttacaatac gctgttccca tcatcaacca gaaaggagag 4801 gtggcaggtc ggctgcacgt ggaggtgatg cgactcagtg gtgatgttgg ggagaggatc 4861 gcaggaggcg atgaggtggc agaggtctcc tttgagaagg agacccagga gaacaaactg 4921 gtgtgcatgg ttaaaatcct gcaagctact gggttgccac agcatctgtc ccactttgtg 4981 ttctgcaaat acagcttctg ggatcaacag gagccggtga ttgtcgctcc tgaagtggac 5041 acctcctcct cttccgtcag caaggagccg cactgcatgg ttgtctttga tcattgcaat 5101 gagttttctg ttaacatcac cgaagacttt atcgagcatc tttccgaagg agcattggca 5161 attgaagtat atggacataa aataaacgat ccccggaaaa accccgccct gtgggatttg 5221 ggaatcatcc aagcaaagac acgtagtctt cgggacagat ggagtgaagt gaccaggaaa 5281 ttggaattct gggttcaaat cttggaacag aatgagaatg gtgaatactg ccctgtagaa 5341 gtgatttctg caaaggatgt cccaacagga ggaatcttcc agctccggca ggggcagtcc 5401 cggagagttc aagtcgaagt gaagtcagtg caggaatctg ggactttacc actgatggaa 5461 gaatgtatac tgtctgttgg cattggatgt gtcaaagtta gaccgctcag agcccccaga 5521 acacatgaga ccttccatga ggaagaggaa gacatggaca gctaccagga tcgagattta 5581 gagagacttc gtagaaaatg gctaaatgca ttaacaaaac gtcaggagta cttggatcaa 5641 caattgcaaa agcttgtcag taaacgtgat aaaacagagg atgatgctga ccgtgaagcg 5701 cagcttctgg agatgcggtt gaccctaact gaggagagga acgcggtgat ggtcccctct 5761 gctggcagtg gtattccagg ggccccagca gaatggaccc cagtacctgg gatggagaca 5821 cacattcctg ttatattcct ggacttaaat gctgatgatt tcagctctca ggataatctt 5881 gatgacccag aagctggtgg atgggatgcg accttgactg gggaagaaga agaggagttc 5941 tttgaattgc agattgtgaa gcagcatgat ggggaggtga aagcagaagc ctcctgggac 6001 tccgcggtgc atggctgccc tcagctcagc aggggcacgc ccgtggacga gcggttgttc 6061 ctgatcgtgc gcgtgacggt ccagctcagc caccctgctg acatgcaact ggtgttacgc 6121 aagagaatct gtgtcaatgt tcacggccgc cagggttttg cacagagtct cctaaaaaag 6181 atgtctcatc gaagttctat tcctggctgt ggagtgactt ttgaaattgt ctccaatatt 6241 ccagaggatg cccagggagt ggaagaacgg gaagcattag caagaatggc agccaatgtt 6301 gaaaacccag cttctgctga ctcggaggct tatattgaaa agtacctcag gagcgtgctg 6361 gctgtagaaa acctcctgac tttagatcgt ctgcgccagg aagttgcagt gaaggaacag 6421 ttaacaggaa aaggaaagtt gagcaggagg agtatcagtt ctccaaatgt gaacagattg 6481 tctggaagcc gacaagatct cattccatca tacagtctag gcagcaacaa gggccggtgg 6541 gaaagtcagc aggatgtatc ccaaaccaca gtttccagag gaatagctcc tgcccccgcc 6601 ctctctgttt ctccccaaaa taaccattct ccagatccag gactcagtaa ccttgcagca 6661 tcctacttga atcctgtcaa atccttcgtg ccgcaaatgc caaagctcct caagtctctc 6721 tttcccgtcc gcgatgagaa gaggggcaag cggccgtctc ccctcgcaca ccagcccgtg 6781 ccccgcatca tggtgcagtc agccagcccg gacatcaggg tgaccaggat ggaggaggct 6841 cagccggaga tgggccctga cgtgctggtg cagacgatgg gggccccggc cttgaagatc 6901 tgcgacaaac ctgccaaagt gccttcccca ccgcctgtca tagctgtcac agcggtcacc 6961 ccggctccgg aggcacagga cgggcccccc agccccctga gtgaagcctc tagcgggtac 7021 ttctcccaca gcgtctccac cgcgaccctg tcggacgccc tgggccccgg cctggacgct 7081 gcggccccgc cggggtccat gcccaccgcc cctgaggccg agcccgaggc gcccatcagc 7141 caccccccac cgcccacggc cgtccccgcc gaggagcccc ctggccccca gcagctcgtg 7201 agccccggtc gggagcgccc cgacctcgag gccccggcgc ccggctcccc gttccgcgtc 7261 cggagggtgc gggcctcgga gttgcgctcc ttctcgcgca tgctggctgg ggaccccggc 7321 tgctccccgg gggccgaggg gaatgcgccg gccccgggcg ccgggggaca ggccctggcc 7381 tctgattccg aggaagctga cgaggtcccg gagtggctcc gagagggcga gttcgtcacc 7441 gtgggcgccc acaaaacggg cgtggtgaga tacgtggggc ctgccgactt ccaagagggc 7501 acgtgggtcg gcgtggagct cgacctgccc tcaggtaaga atgacggttc catcggcggg 7561 aagcagtact tcaggtgtaa ccctggctac gggctgctgg tcaggcccag ccgggtccgc 7621 agggccacgg gccctgtgcg gcggcgcagc acaggactcc ggctgggtgc ccccgaggcc 7681 cgccggagcg ccaccctctc gggctccgcc accaacctgg cctcgctgac agctgccctg 7741 gccaaggccg acaggagcca caagaaccct gagaaccgga aatcctgggc cagcggatcc 7801 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 7861 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 7921 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 7981 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 8041 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 8101 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 8161 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 8221 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 8281 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 8341 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 8401 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 8461 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 8521 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 8581 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 8641 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 8701 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 8761 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 8821 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 8881 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 8941 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 9001 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 9061 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 9121 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 9181 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 9241 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 9301 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 9361 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 9421 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 9481 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 9541 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 9601 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 9661 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 9721 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 9781 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 9841 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 9901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 9961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 10021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 10081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 10141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 10201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 10261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 10321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 10381 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 10441 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 10501 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 10561 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 10621 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 10681 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 10741 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 10801 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 10861 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 10921 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 10981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 11041 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 11101 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 11161 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 11221 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 11281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 11341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 11401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 11461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 11521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 11581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 11641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 11701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 11761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 11821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 11881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 11941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 12001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 12061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 12121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 12181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 12241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 12301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 12361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 12421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 12481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 12541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 12601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 12661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 12721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 12781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 12841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 12901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 12961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 13021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 13081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 13141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 13201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 13261 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 13321 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 13381 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 13441 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 13501 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 13561 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 13621 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 13681 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 13741 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.