pTac-GST-PADI1 plasmid

pTac-GST-PADI1 plasmid

PVT95900

pTac-GST-PADI1 plasmid Information

Plasmid name: pTac-GST-PADI1(human)
Alias: NM_013358.3;HPAD10; PAD1; PDI; PDI1
Gene lenght: 1992bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-PADI1 plasmid Description

pTac-GST-PADI1 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-PADI1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atggccccaa 961 agagagttgt gcagctgtcc ctgaagatgc ctacccatgc cgtgtgtgtg gtgggagtcg 1021 aggcacatgt ggacattcac agtgatgtgc ccaagggtgc caacagcttc agggtctctg 1081 gaagctccgg ggtggaggtc ttcatggtct acaaccgcac acgtgtgaaa gagcccatag 1141 gcaaggcccg ttggccgcta gacactgatg cagacatggt cgtatctgtg ggcacagcca 1201 gtaaggaatt aaaggacttc aaggtgaggg tctcctactt tggggagcag gaagaccaag 1261 ctctgggccg cagcgtgctt tacctcactg gcgtcgatat ttcccttgag gttgacacag 1321 gccgcacagg caaggtgaag aggagccaag gggacaagaa aacctggcgc tggggccctg 1381 agggctatgg ggctatcttg ctggtgaact gtgaccggga caatcacagg tccgcagagc 1441 ctgacctcac ccacagctgg ctgatgtcgc tggctgacct gcaggacatg tccccaatgc 1501 tgctgagctg caatggcccc gacaagctct tcgacagcca caagcttgtc ttgaacgtgc 1561 ccttttctga ttccaaaaga gtgagggtct tctgtgccag gggtgggaat tctctctcgg 1621 actacaaaca ggtgctgggg ccccagtgtc tgtcctatga agttgagcga cagccagggg 1681 agcaggagat caagttctat gtggaggggc tgaccttccc cgatgccgat ttcctagggc 1741 tggtttccct cagtgtcagc ctggtggacc cggggaccct gcccgaggtg accctcttca 1801 cagacactgt gggcttccgc atggccccct ggatcatgac gcccaacact cagcctcctg 1861 aggagctgta tgtgtgcaga gtgatggaca ctcatggctc caatgagaaa ttcctggagg 1921 acatgtctta tctgacattg aaagccaact gcaagctgac catctgccct caagttgaaa 1981 atcgaaatga ccgctggatc caggacgaga tggagtttgg ctacatcgag gcccctcaca 2041 aatccttccc cgtggtcttt gactccccca ggaacagggg cctgaaagat ttcccctata 2101 agaggatcct gggtcctgac tttggatatg ttacccggga gatcccgctc cctggtccct 2161 ccagccttga ctccttcggc aacctggacg tcagcccgcc cgtcacggtg ggcggcacgg 2221 aataccccct gggccggatc ctcatcggga gcagcttccc caagtccggt gggcggcaga 2281 tggccagggc agtgcggaac ttcctgaagg cacagcaggt gcaggcaccc gtggagctct 2341 actcggactg gctctctgtg ggccatgtgg acgagtttct gacctttgtg cctacctctg 2401 accaaaaggg cttccggctg ctcctggcta gccccagcgc ttgcctcaaa ctcttccaag 2461 agaagaaaga agagggttat ggggaggcag cccagtttga tgggttaaaa caccaggcaa 2521 aaagaagcat taatgagatg ctggcagaca gacacctcca gagagacaat cttcatgcac 2581 agaaatgcat tgactggaac cgtaatgtgc tgaagcggga gctgggcctg gcagagagtg 2641 acatcgtgga cattccccag ctcttcttcc tgaaaaactt ctacgcggaa gccttcttcc 2701 cagacatggt taacatggtg gtcttaggca agtacctggg catccccaag ccctacgggc 2761 ccatcatcaa tggccgctgc tgcctggagg agaaggtgca gtccctgctg gagcctctgg 2821 gcctgcactg catcttcatt gatgactact tgtcctacca cgagctgcag ggggagatcc 2881 actgtggcac caacgtgcgc aggaagccct ttcccttcaa atggtggaac atggtgccct 2941 gactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg 3001 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 3061 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 3121 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg 3181 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3241 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3301 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3361 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 3421 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 3481 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 3541 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 3601 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 3661 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 3721 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 3781 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 3841 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 3901 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 3961 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 4021 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 4081 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 4141 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 4201 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 4261 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 4441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 4501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 4561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 4621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 4681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 4741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 4801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 4861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 4921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 4981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 5041 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 5101 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 5161 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 5221 taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg 5281 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt 5341 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg 5401 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg 5461 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg 5521 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg 5581 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc 5641 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg 5701 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg 5761 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg 5821 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg 5881 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc 5941 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa 6001 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg 6061 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg 6121 gatacgacga taccgaagac agctcatgtt atatcccgcc gtcaaccacc atcaaacagg 6181 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 6241 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc 6301 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 6361 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 6421 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 6481 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt 6541 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 6601 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 6661 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt 6721 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa 6781 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt 6841 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt 6901 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.