pCMV-3×FLAG-JMJD4-Neo plasmid

pCMV-3×FLAG-JMJD4-Neo plasmid

PVT94178

pCMV-3×FLAG-JMJD4-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-JMJD4(human)-Neo
Alias: NM_023007.3
Gene lenght: 1254bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-JMJD4-Neo plasmid Description

pCMV-3×FLAG-JMJD4-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-JMJD4-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggaccgcg agacgcgcgc cctcgccgac 1021 agccacttcc gaggcctggg ggtcgatgtc cccggcgtcg gccaggctcc gggccgggta 1081 gccttcgtct cggagccggg cgccttctcc tacgccgact ttgtgcgggg cttcttgctg 1141 cccaacctgc cctgcgtgtt ttccagcgcc ttcacgcagg gctggggcag ccggcggcgc 1201 tgggtgacgc ccgcggggag gcccgacttc gaccacctgc tacggaccta cggagacgtg 1261 gttgtaccag ttgcaaactg tggggtccag gaatacaact cgaaccccaa agagcacatg 1321 actctcagag actacatcac ctactggaaa gagtacatac aggcgggcta ctcctctccc 1381 aggggctgtc tctacctcaa agactggcac ttgtgcaggg actttccggt ggaggacgtt 1441 ttcaccctgc ctgtgtactt ctcgtccgac tggctgaatg agttctggga tgcactggat 1501 gtggatgact accgctttgt ctacgcgggg cctgcgggca gctggtcccc gttccatgct 1561 gacatcttcc gctccttcag ctggtctgtc aatgtctgtg ggaggaagaa gtggctcctc 1621 ttccccccag ggcaggaaga ggccctgcgg gaccgccacg gcaacctgcc ctacgacgtg 1681 acctccccag cactctgcga cacacacctg cacccacgga accagcttgc tggcccaccc 1741 ttggagatca cgcaggaagc gggcgagatg gtgtttgtgc ccagtggctg gcaccaccag 1801 gtgcacaacc tggatgacac catctccatc aaccacaact gggtcaatgg cttcaacctg 1861 gccaacatgt ggcgcttctt gcagcaggag ctatgcgccg tgcaggagga ggtcagcgag 1921 tggagggact ccatgcccga ctggcaccac cactgccagg tcatcatgag gtcctgctcg 1981 ggcatcaact ttgaagagtt ttaccacttc ctcaaggtca tcgctgagaa gaggctcctg 2041 gtcctgaggg aggcagccgc tgaggacggt gctgggttgg gtttcgaaca ggcagccttt 2101 gatgttgggc gcatcacaga ggtgctggcc tccttggttg cgcaccccga cttccagaga 2161 gtggacacca gcgcgttctc accacagccc aaagagctgc tgcagcagct gagagaggct 2221 gttgatgctg ctgcggcccc ataggaattc tgcagatatc cagcacagtg gcggccgctc 2281 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 2341 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 2401 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 2461 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 2521 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 2581 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 2641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 2701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 2761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 2821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 2881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 2941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 3001 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 3061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 3121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 3181 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 3241 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 3301 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 3361 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat 3421 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 3481 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 3541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 3601 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 3661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 3721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 3781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 3841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 3901 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca 3961 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 4021 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 4081 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 4141 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 4201 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 4261 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 4321 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 4381 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 4441 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 4501 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 4561 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 4621 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 4681 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 4741 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 4801 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 4861 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 4921 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 4981 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 5041 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 5101 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 5161 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 5221 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 5281 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 5341 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 5401 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 5461 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa 5521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 5581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 5641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 5701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 5761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 5821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 5881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 5941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 6001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 6061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 6121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 6181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 6241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 6301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 6361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 6421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 6481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 6541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 6601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 6661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 6721 c

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.