pLV3-CMV-HA-WSB1-HA-CopGFP-Pur

pLV3-CMV-HA-WSB1-HA-CopGFP-Puro plasmid

PVT95892

pLV3-CMV-HA-WSB1-HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HA-WSB1(human)-HA-CopGFP-Puro
Alias: NM_015626.10;SWIP1; WSB-1
Gene lenght: 1263bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HA-WSB1-HA-CopGFP-Puro plasmid Description

pLV3-CMV-HA-WSB1-HA-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HA-WSB1-HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgT ACCCATACGA TGTTCCAGAT 2341 TACGCTatgg ccagctttcc cccgagggtc aacgagaaag agatcgtgag attacgtact 2401 ataggtgaac ttttagctcc tgcagctcct tttgacaaga aatgtggtcg tgaaaattgg 2461 actgttgctt ttgctccaga tggttcatac tttgcttggt cacaaggaca tcgcacagta 2521 aagcttgttc cgtggtccca gtgccttcag aactttctct tgcatggcac caagaatgtt 2581 accaattcaa gcagtttaag attgccaaga caaaatagtg atggtggtca gaaaaataag 2641 cctcgtgaac atattataga ctgtggagat atagtctgga gtcttgcttt tgggtcatca 2701 gttccagaaa aacagagtcg ctgtgtaaat atagaatggc atcgcttcag atttggacaa 2761 gatcagctac ttcttgctac agggttgaac aatgggcgta tcaaaatatg ggatgtatat 2821 acaggaaaac tcctccttaa cttggtagat catactgaag tggtcagaga tttaactttt 2881 gctccagatg gaagcttgat cctggtgtca gcttcaagag acaaaactct cagagtatgg 2941 gacctgaaag atgatggaaa catgatgaaa gtattgaggg ggcatcagaa ttgggtgtac 3001 agctgtgcat tctctcctga ctcttctatg ctgtgttcag tcggagccag taaagcagtt 3061 ttcctttgga atatggataa atacaccatg atacggaaac tagaaggaca tcaccatgat 3121 gtggtagctt gtgacttttc tcctgatgga gcattactgg ctactgcatc ttatgatact 3181 cgagtatata tctgggatcc acataatgga gacattctga tggaatttgg gcacctgttt 3241 cccccaccta ctccaatatt tgctggagga gcaaatgacc ggtgggtacg atctgtatct 3301 tttagccatg atggactgca tgttgcaagc cttgctgatg ataaaatggt gaggttctgg 3361 agaattgatg aggattatcc agtgcaagtt gcacctttga gcaatggtct ttgctgtgcc 3421 ttctctactg atggcagtgt tttagctgct gggacacatg acggaagtgt gtatttttgg 3481 gccactccac ggcaggtccc tagcctgcaa catttatgtc gcatgtcaat ccgaagagtg 3541 atgcccaccc aagaagttca ggagctgccg attccttcca agcttttgga gtttctctcg 3601 tatcgtattT ACCCATACGA TGTTCCAGAT TACGCTtagg atccCTGCGA TATCCAGCgc 3661 ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 3721 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 3781 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 3841 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 3901 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 3961 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 4021 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 4081 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 4141 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 4201 gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg 4261 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 4321 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 4381 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 4441 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 4501 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 4561 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 4621 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 4681 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 4741 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 4801 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 4861 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 4921 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 4981 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga 5041 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 5101 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 5161 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 5221 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 5281 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 5341 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 5401 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 5461 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 5521 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 5581 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc 5641 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5701 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5761 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5821 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5881 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5941 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 6001 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 6061 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6121 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc 6181 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac 6241 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 6301 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 6361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6421 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 6481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 6541 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 6601 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 6661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 6721 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 6781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 6841 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 6901 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6961 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7021 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7081 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7501 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7561 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7621 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7681 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7741 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7801 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8041 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8101 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8161 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8221 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8281 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8341 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8821 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8881 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9061 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9121 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9181 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9241 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9301 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9361 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9421 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9481 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 9541 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.