pCMV-Bag6-3×Myc-Neo plasmid

pCMV-Bag6-3×Myc-Neo plasmid

PVT95890

pCMV-Bag6-3×Myc-Neo plasmid Information

Plasmid name: pCMV-Bag6(mouse)-3×Myc-Neo
Alias: NM_057171.3;2410045D21Rik; BAG-6; Bat3; D17H6S52E; G3;Scythe
Gene lenght: 3462bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Bag6-3×Myc-Neo plasmid Description

pCMV-Bag6-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Bag6-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgga gccgagtgat 721 agtgccagta ccgctatgga ggagcctgac agcctggagg tactggtgaa gaccctggac 781 tctcagactc ggacttttat tgtgggggcc cagatgaatg taaaggagtt taaggaacac 841 atagctgcct ctgtcagcat cccttccgag aaacagcggc tcatctacca gggccgggtc 901 ctacaagacg acaagaagct ccaggagtac aacgttgggg gaaaggttat tcacctggtg 961 gaacgggctc ctcctcagac tcagcttcct tctggagcat cttctgggac aggctctgcc 1021 tcagcaactc atggtggggc acccctgcct ggcactcggg gccctggggc ttctgttcat 1081 gaccggaatg ccaacagcta tgtcatggtt ggaaccttca atcttcctag tgacggctct 1141 gctgtggatg ttcacatcaa catggaacag gccccaattc agagtgagcc ccgggtccgg 1201 ctggtgatgg ctcagcacat gattagggat atacagacct tactgtcccg gatggagtgt 1261 cgagggggaa cccaagctca ggccagtcag ccacccccgc agacaccgca gactgtggcc 1321 tcagagacgg tagccttgaa ctcacaaaca tcagaaccag tcgaaagtga agctcctcct 1381 cgagagccca tggagtcaga agaaatggag gaacgtcccc caacccagac tccagagctt 1441 gcgccctcag gcccagctcc cgcaggccca gctcccgcag gcccagctcc cgcgccagag 1501 acaaatgcac ccaaccaccc ttcccccgca gagcacgtgg aggtgctcca ggagcttcag 1561 cgcttgcagc gccgtcttca gcctttccta cagcgctact gcgaggtcct gggcgctgct 1621 gccaccacag actacaacaa caaccatgag ggccgtgagg aggaccagag gttgatcaac 1681 ttggttgggg agagcctgcg gctactgggc aacactttcg tggcattgtc tgatctgcgc 1741 tgcaatctag cctgtgcacc cccacggcac ctgcacgtgg ttcggcctat gtctcactac 1801 acgactccca tggtgctcca gcaggcagcc attcccattc agatcaatgt ggggactact 1861 gtgaccatga caggcaatgg ggctcggcct ccaccagctc ctggtgcgga ggcagcaacc 1921 ccaggttctg cccaggccac atccctgcct ccctcttcca ccactgttga ttcatcaact 1981 gaaggagctc ccccaccagg gccagcaccg ccaccagcct ccagccaccc acgggtcatc 2041 cggatttccc accagagtgt ggagcccgtg gtcatgatgc acatgaacat tcaagattct 2101 ggagcacagc ctggtggtgt gccaagtgct cccactggtc cactgggacc tcctggtcat 2161 ggacagaccc tgggacagca ggtgcccggc ttcccaacag caccaactcg ggtggtgatt 2221 gcccggccaa ctcctccaca ggctcggcct tcccatcctg ggggacctcc agtctctgga 2281 gctctgcagg gcgctgggct gggtacaaac acttcattgg cccagatggt gagcggcctt 2341 gtggggcaac ttcttatgca gcctgttctt gtggctcagg ggactccagg aatggctcag 2401 gctcaggccc aggcccaggc tcaggcccag gcccaggccc aggctccagc tccggctccg 2461 gctccagctc cagctcctgc cactgcttca gctagtgctg gtactaccaa cacagctacc 2521 acagctggcc ctgctcctgg gggtcctgcc cagcctccac ctcctcagcc ctctgcagct 2581 gatcttcagt tctctcagct cctgggaaat ctgctggggc ctgcagggcc aggggctggg 2641 gggccaggca tggcttctcc caccatcact gtggcaatgc ctggtgtccc tgcttttctc 2701 cagggcatga ctgatttttt gcaggcatca cagactgccc ctccaccccc tccacctcct 2761 ccacccccac cccctgcccc agagcagcag agcacacccc caccagggtc tccttctggt 2821 ggaacagcaa gccctggagg cttaggtcct gagagcctgc caccagagtt tttcacctca 2881 gtggtgcagg gcgtgttgag ctccctcctg ggctccttgg gggctcgagc tggcagcagt 2941 gagagtatcg ctgccttcat ccaacgcctc agtggatcca gcaacatctt tgagcctggg 3001 gctgatggcg cccttggatt cttcggagct ctgctctctc tcctgtgcca gaatttctcc 3061 atggtggatg tggtgatgct tctccatggc catttccagc cactgcagcg gctccagcca 3121 cagctgcgat ctttcttcca ccagcactac ctgggtggcc aggagcccac gcctagcaac 3181 atccggatgg cgacccacac actgatcact gggctggaag agtatgtaag ggagagtttt 3241 tctttggtac aggttcagcc aggtgtggat atcatccgga caaatttaga atttctccaa 3301 gagcagttta acagcattgc tgctcatgtg ctgcactgta cagacagtgg atttggagcc 3361 cggttgctgg agctgtgtaa ccagggcctg tttgaatgct tggccctgaa cctccactgc 3421 ttggggggac agcaaatgga gcttgctgct gtcatcaatg gtcgaattcg ccgcatgtct 3481 cgcggggtga atccatcctt ggtgagctgg ctgacaacca tgatgggact gaggcttcag 3541 gtggtcttgg agcacatgcc tgtgggtccc gacgccatcc tcagatatgt tcgtagggtt 3601 ggtgatcctc ctcagacact tcctgaagag ccgatggaag ttcagggagc agaaagaact 3661 tcccctgaac ctcagagaga gaatgcttcc ccagcccctg gaacaacagc agaagaagcc 3721 atgtcccgag gcccgccccc tgctcctgaa ggaggttccc gagatgaaca ggatggagct 3781 tcagctgatg cagaaccctg ggcagctgca gtcccccctg aatgggtccc tattatccag 3841 caggacattc agagccagcg gaaggtgaaa cctcagccgc ccctgagtga tgcctacctc 3901 agtggcatgc ctgccaagag acgaaagaca atgcagggtg agggccccca gctgctactc 3961 tcagaggcag tgagccgggc agctaaggca gccggagctc ggcccctgac aagccccgag 4021 agcctgagcc gggacctgga ggcaccagag gttcaggaga gctacaggca gcagctccgg 4081 tctgatatcc aaaaacgact gcaggaagat cccaactaca gcccccagcg cttccctaat 4141 gcccatcggg catttgctga tgaccccCTC GAGGAACAAA AACTAATATC AGAGGAAGAT 4201 TTGGAACAGA AACTAATCAG TGAAGAAGAT TTAGAGCAAA AGTTAATTTC CGAGGAGGAC 4261 TTATAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 4321 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 4381 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 4441 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 4501 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 4561 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 4621 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 4681 AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 4741 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 4801 CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT 4861 GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC 4921 GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT 4981 CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA 5041 GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT 5101 CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC 5161 TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 5221 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 5281 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 5341 ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 5401 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 5461 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 5521 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT 5581 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA 5641 GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 5701 CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 5761 AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC 5821 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA 5881 TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT 5941 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA 6001 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC 6061 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG 6121 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC 6181 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC 6241 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG 6301 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA 6361 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG 6421 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG 6481 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT 6541 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC 6601 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG 6661 GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA 6721 GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC 6781 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC 6841 GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 6901 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 6961 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 7021 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 7081 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 7141 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 7201 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 7261 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 7321 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 7381 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 7441 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 7501 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 7561 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 7621 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 7681 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 7741 CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 7801 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 7861 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 7921 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 7981 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 8041 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 8101 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 8161 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 8221 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 8281 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 8341 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 8401 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 8461 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 8521 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 8581 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 8641 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 8701 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 8761 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 8821 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 8881 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 8941 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 9001 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 9061 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 9121 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 9181 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 9241 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 9301 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC 9361 GACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG 9421 TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT 9481 GACCGACAAT TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.