pLV3-CMV-Cd14-Hyg plasmid

pLV3-CMV-Cd14-Hyg plasmid

PVT95828

pLV3-CMV-Cd14-Hyg plasmid Information

Plasmid name: pLV3-CMV-Cd14(mouse)-Hyg
Alias: NM_009841.4
Gene lenght: 1101bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cd14-Hyg plasmid Description

pLV3-CMV-Cd14-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Cd14-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagccacca tggagcgtgt gcttggcttg ttgctgttgc ttctggtgca cgcctctccc 2341 gccccaccag agccctgcga gctagacgag gaaagttgtt cctgcaactt ctcagatccg 2401 aagccagatt ggtccagcgc tttcaattgt ttgggggcgg cagatgtgga attgtacggc 2461 ggcggccgca gcctggaata ccttctaaag cgtgtggaca cggaagcaga tctggggcag 2521 ttcactgata ttatcaagtc tctgtcctta aagcggctta cggtgcgggc cgcgcggatt 2581 cctagtcgga ttctattcgg agccctgcgt gtgctcggga tttccggcct ccaggaactg 2641 actcttgaaa atctcgaggt aaccggcacc gcgccgccac cgcttctgga agccaccgga 2701 cccgatctca acatcttgaa cctccgcaac gtgtcgtggg caacaaggga tgcctggctc 2761 gcagaactgc agcagtggct aaagcctgga ctcaaggtac tgagtattgc ccaagcacac 2821 tcgctcaact tttcctgcga acaggtccgc gtcttccctg ccctctccac cttagacctg 2881 tctgacaatc ctgaattggg cgagagagga ctgatctcag ccctctgtcc cctcaagttc 2941 ccgaccctcc aagttttagc gctgcgtaac gcggggatgg agacgcccag cggcgtgtgc 3001 tctgcgctgg ccgcagcaag ggtacagctg caaggactag accttagtca caattcactg 3061 cgggatgctg caggcgctcc gagttgtgac tggcccagtc agctaaactc gctcaatctg 3121 tctttcactg ggctgaagca ggtacctaaa gggctgccag ccaagctcag cgtgctggat 3181 ctcagttaca acaggctgga taggaaccct agcccagatg agctgcccca agtggggaac 3241 ctgtcactta aaggaaatcc ctttttggac tctgaatccc actcggagaa gtttaactct 3301 ggcgtagtca ccgccggagc tccatcatcc caagcagtgg ccttgtcagg aactctggct 3361 ttgctcctag gagatcgcct ctttgtttaa ggatccgcgg ccgcaaggat ctgcgatcgc 3421 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3481 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3541 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3601 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3661 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3721 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3781 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3841 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 3901 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gtcgacatga 3961 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg 4021 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag 4081 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt 4141 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg 4201 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag 4261 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga 4321 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg 4381 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact 4441 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga 4501 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca 4561 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt 4621 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta 4681 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc 4741 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca 4801 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg 4861 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg 4921 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat 4981 aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5041 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5101 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5161 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5221 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 5281 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 5341 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 5401 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 5461 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 5521 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 5581 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5641 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 5701 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 5761 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 5821 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 5881 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 5941 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6061 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 6121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 6181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 6241 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6481 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 8461 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 8521 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 8581 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 8641 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 8701 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 8761 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 8821 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 8881 ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.