pCMV-TFRC-IRES2-EGFP-Neo plasm

pCMV-TFRC-IRES2-EGFP-Neo plasmid

PVT95806

pCMV-TFRC-IRES2-EGFP-Neo plasmid Information

Plasmid name: pCMV-TFRC(human)-IRES2-EGFP-Neo
Alias: NM_003234.4;CD71; IMD46; p90; T9; TFR; TFR1; TR; TRFR
Gene lenght: 2283bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-TFRC-IRES2-EGFP-Neo plasmid Description

pCMV-TFRC-IRES2-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TFRC-IRES2-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagGC CACCatgatg gatcaagcta gatcagcatt ctctaacttg 661 tttggtggag aaccattgtc atatacccgg ttcagcctgg ctcggcaagt agatggcgat 721 aacagtcatg tggagatgaa acttgctgta gatgaagaag aaaatgctga caataacaca 781 aaggccaatg tcacaaaacc aaaaaggtgt agtggaagta tctgctatgg gactattgct 841 gtgatcgtct ttttcttgat tggatttatg attggctact tgggctattg taaaggggta 901 gaaccaaaaa ctgagtgtga gagactggca ggaaccgagt ctccagtgag ggaggagcca 961 ggagaggact tccctgcagc acgtcgctta tattgggatg acctgaagag aaagttgtcg 1021 gagaaactgg acagcacaga cttcaccggc accatcaagc tgctgaatga aaattcatat 1081 gtccctcgtg aggctggatc tcaaaaagat gaaaatcttg cgttgtatgt tgaaaatcaa 1141 tttcgtgaat ttaaactcag caaagtctgg cgtgatcaac attttgttaa gattcaggtc 1201 aaagacagcg ctcaaaactc ggtgatcata gttgataaga acggtagact tgtttacctg 1261 gtggagaatc ctgggggtta tgtggcgtat agtaaggctg caacagttac tggtaaactg 1321 gtccatgcta attttggtac taaaaaagat tttgaggatt tatacactcc tgtgaatgga 1381 tctatagtga ttgtcagagc agggaaaatc acctttgcag aaaaggttgc aaatgctgaa 1441 agcttaaatg caattggtgt gttgatatac atggaccaga ctaaatttcc cattgttaac 1501 gcagaacttt cattctttgg acatgctcat ctggggacag gtgaccctta cacacctgga 1561 ttcccttcct tcaatcacac tcagtttcca ccatctcggt catcaggatt gcctaatata 1621 cctgtccaga caatctccag agctgctgca gaaaagctgt ttgggaatat ggaaggagac 1681 tgtccctctg actggaaaac agactctaca tgtaggatgg taacctcaga aagcaagaat 1741 gtgaagctca ctgtgagcaa tgtgctgaaa gagataaaaa ttcttaacat ctttggagtt 1801 attaaaggct ttgtagaacc agatcactat gttgtagttg gggcccagag agatgcatgg 1861 ggccctggag ctgcaaaatc cggtgtaggc acagctctcc tattgaaact tgcccagatg 1921 ttctcagata tggtcttaaa agatgggttt cagcccagca gaagcattat ctttgccagt 1981 tggagtgctg gagactttgg atcggttggt gccactgaat ggctagaggg atacctttcg 2041 tccctgcatt taaaggcttt cacttatatt aatctggata aagcggttct tggtaccagc 2101 aacttcaagg tttctgccag cccactgttg tatacgctta ttgagaaaac aatgcaaaat 2161 gtgaagcatc cggttactgg gcaatttcta tatcaggaca gcaactgggc cagcaaagtt 2221 gagaaactca ctttagacaa tgctgctttc cctttccttg catattctgg aatcccagca 2281 gtttctttct gtttttgcga ggacacagat tatccttatt tgggtaccac catggacacc 2341 tataaggaac tgattgagag gattcctgag ttgaacaaag tggcacgagc agctgcagag 2401 gtcgctggtc agttcgtgat taaactaacc catgatgttg aattgaacct ggactatgag 2461 aggtacaaca gccaactgct ttcatttgtg agggatctga accaatacag agcagacata 2521 aaggaaatgg gcctgagttt acagtggctg tattctgctc gtggagactt cttccgtgct 2581 acttccagac taacaacaga tttcgggaat gctgagaaaa cagacagatt tgtcatgaag 2641 aaactcaatg atcgtgtcat gagagtggag tatcacttcc tctctcccta cgtatctcca 2701 aaagagtctc ctttccgaca tgtcttctgg ggctccggct ctcacacgct gccagcttta 2761 ctggagaact tgaaactgcg taaacaaaat aacggtgctt ttaatgaaac gctgttcaga 2821 aaccagttgg ctctagctac ttggactatt cagggagctg caaatgccct ctctggtgac 2881 gtttgggaca ttgacaatga gttttaagaa ttctgcagtc gacggtaccg cgggcccggg 2941 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc 3001 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg 3061 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc 3121 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga 3181 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg 3241 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag 3301 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc 3361 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct 3421 cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac 3481 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag 3541 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 3601 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 3661 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 3721 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 3781 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 3841 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 3901 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 3961 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 4021 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 4081 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 4141 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 4201 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg 4261 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 4321 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 4381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 4441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc 4501 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 4561 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 4621 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 4681 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 4741 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 4801 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 4861 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 4921 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 4981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 5041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga 5101 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 5161 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 5221 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 5281 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 5341 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 5401 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga 5461 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 5521 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 5581 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 5641 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 5701 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 5761 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 5821 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 5881 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 5941 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 6001 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 6061 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 6121 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 6181 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 6241 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 6301 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 6361 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 6421 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag 6481 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt 6541 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 6601 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 6661 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 6721 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 6781 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 6841 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 6901 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 6961 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 7021 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 7081 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 7141 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 7201 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 7261 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 7321 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 7381 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 7441 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 7501 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 7561 gtggataacc gtattaccgc catgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.