pCMV-BRD8-Neo plasmid

pCMV-BRD8-Neo plasmid

PVT95589

pCMV-BRD8-Neo plasmid Information

Plasmid name: pCMV-BRD8(human)-Neo
Alias: NM_139199.2;p120; SMAP; SMAP2
Gene lenght: 3708bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-BRD8-Neo plasmid Description

pCMV-BRD8-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-BRD8-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gcgacgggaa cgggcaaaca caagctgcta agcactggcc ccacagagcc 961 atggtccatc cgagagaagc tatgtttagc atcttctgtc atgagaagtg gcgatcaaaa 1021 ttgggtatca gttagcagag caatcaagcc ctttgcagaa cctggccgcc ctccagactg 1081 gttctctcaa aaacattgtg cttcccagta ctcggagctt ttagagacca ctgagacacc 1141 aaaacggaaa cgaggtgaaa agggagaagt ggtggaaact gttgaagatg ttattgttcg 1201 gaaattgact gctgagcgag ttgaagaact aaagaaagtg ataaaggaaa cccaggagag 1261 atatagacgg ctaaagagag atgcagaact aattcaagct ggacacatgg acagcagact 1321 ggatgagctt tgcaatgaca ttgcaacgaa aaagaaattg gaagaagagg aggctgaagt 1381 aaagaggaag gctacagatg ctgcatacca ggctcgtcaa gcagtaaaaa cacccccccg 1441 gaggttaccc actgtgatgg ttcgctctcc tatagattct gcctccccag gaggtgatta 1501 tccacttggg gacttgactc caaccactat ggaagaggct acctctgggg tcaatgagag 1561 tgaaatggct gtggcttctg gccacctgaa cagtacaggt gtcctcctgg aggtaggcgg 1621 ggtccttccc atgatacatg gtggggagat acagcaaaca cccaatactg ttgcagcctc 1681 ccctgctgca tcaggtgctc ccactctttc ccggctttta gaagctggtc ctacacagtt 1741 caccacacct cttgcttcct tcactactgt tgccagtgag cctccagtta aacttgtgcc 1801 accccctgta gagtctgtgt cccaagctac cattgtcatg atgcctgcgc tgccagcacc 1861 atcctctgct ccggctgtct ccactactga aagtgtagct ccagtgagtc aacccgacaa 1921 ctgtgttccc atggaggctg tgggggatcc acatactgtg actgtttcca tggacagcag 1981 tgaaatatcc atgatcatca attctatcaa agaagagtgt tttcgatcag gggtagcaga 2041 ggctcctgtt ggatcaaagg ctcccagcat agatgggaag gaagaattag atctggctga 2101 gaagatggat attgctgtgt cttacacagg tgaagagctg gattttgaga ctgttggaga 2161 catcattgcc atcattgagg acaaggtaga tgatcatcct gaagtgctgg atgtggcagc 2221 agtggaagca gcactgtcat tttgtgaaga aaatgatgat cctcagtccc tgcctggccc 2281 ctgggagcat cctatccagc aggagcggga caagccagta cctctccctg caccagaaat 2341 gacggtcaag caagagagac tggactttga ggaaacggaa aacaagggaa tacatgaact 2401 ggtggacatc agggagccca gtgcagagat caaggtggaa cctgcagaac cagagccagt 2461 catttcagga gccgaaatag tagctggagt tgttccagcc acaagtatgg agccaccaga 2521 actcaggagt caggacttag atgaggaact gggaagtact gcagctggag agattgttga 2581 agcagatgtt gccattggga aaggcgatga gactccactt acaaatgtga agacagaggc 2641 atcccctgaa agcatgttgt ctccatcaca tggctcaaat cccattgaag atcctttaga 2701 ggcagagact cagcacaagt ttgaaatgtc agactcattg aaagaagaat cagggactat 2761 ttttggaagc cagataaagg atgccccagg tgaggatgag gaggaagatg gtgtcagtga 2821 agcggccagc ctagaggagc ctaaggaaga ggatcaagga gaaggctact tgtcagaaat 2881 ggataatgaa cctcctgtga gcgagagtga tgatggcttc agcatacaca atgctacact 2941 gcagtcacac acactggcag actccatccc cagcagccct gcttcttcac agttctctgt 3001 ctgtagtgag gatcaggaag ctattcaggc acagaaaatt tggaagaaag ccatcatgct 3061 tgtatggaga gctgcagcta atcataggta tgccaatgtc ttcctgcagc ctgttacaga 3121 tgacatagca cctggctacc acagcattgt gcagaggcct atggatttgt caactattaa 3181 gaaaaacata gaaaatggac tgatccgaag cacagctgaa tttcagcgtg acattatgct 3241 gatgtttcag aatgctgtaa tgtacaatag ctcagaccat gatgtctatc acatggcagt 3301 ggagatgcag cgagatgtct tggaacagat ccagcaattc ttggccacgc agttgattat 3361 gcaaacatcc gagtctggga tcagtgctaa aagtcttcga gggagagatt ctacccgcaa 3421 acaggatgct tcagagaagg acagtgtccc aatgggctct cctgccttcc ttctctctct 3481 ctttatggga cacgagtggg tttggctgga ttctgaacaa gatcatccca atgactctga 3541 gttgagcaat gactgcaggt ccctcttcag ctcatgggac tccagtctgg atcttgatgt 3601 gggcaactgg agggaaactg aggatccaga ggctgaggaa ctagaggaaa gcagcccgga 3661 gagagaacct agtgaactgc ttgttgggga tggaggcagt gaggaatctc aggaagcggc 3721 aaggaaagcc agccaccaga acctcctcca ctttctctct gaggtagctt atttaatgga 3781 gcccttgtgc atcagcagca acgaatcaag tgaaggctgc tgccctccat ctggtaccag 3841 acaagaagga agggaaatta aagctagcga aggagaaagg gagctctgca gagagactga 3901 agagctttca gctaaaggag accccttagt agctgaaaag ccactgggag aaaatggaaa 3961 gccagaggtg gcttcagctc cctcagttat ttgtacagtt cagggactac tcacagagag 4021 tgaagagggg gaggctcagc aagaatccaa aggggaggac cagggtgaag tatatgtgtc 4081 agagatggaa gaccagcccc cttcaggcga gtgtgatgat gcctttaaca ttaaggagac 4141 tcccttggtg gatacacttt tcagccatgc tacctcctca aagctgactg atctaagcca 4201 ggatgaccct gttcaggatc atttgctatt taagaagact ctcctgccag tctggaagat 4261 gattgccagt cacaggttca gcagtccatt tctgaagcct gtgtcagaaa ggcaggcccc 4321 agggtacaag gatgtggtga aaagacccat ggacttaact agcctgaaga gaaatctctc 4381 taagggtcgg attcgcacca tggcccaatt cctgcgagac ctgatgctga tgttccaaaa 4441 tgctgtaatg tacaatgact ctgatcatca tgtataccat atggctgtgg agatgcggca 4501 agaagtcctg gagcagattc aggtactgaa tatctggtta gacaaaagaa aaggctcaag 4561 tagtctggaa ggagaaccag ctaacccagt ggatgatgga aaacctgttt tctaaCTCGA 4621 GTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 4681 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 4741 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 4801 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 4861 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG 4921 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 4981 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 5041 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 5101 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 5161 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 5221 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 5281 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 5341 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 5401 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 5461 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 5521 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 5581 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 5641 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 5701 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 5761 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 5821 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 5881 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 5941 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 6001 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 6061 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 6121 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 6181 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 6241 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 6301 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 6361 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 6421 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 6481 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 6541 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 6601 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 6661 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 6721 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 6781 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 6841 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 6901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 6961 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 7021 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 7081 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 7141 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 7201 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 7261 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 7321 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 7381 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 7441 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 7501 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 7561 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 7621 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 7681 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 7741 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 7801 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 7861 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 7921 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 7981 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 8041 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 8101 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 8161 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 8221 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 8281 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 8341 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 8401 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 8461 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 8521 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 8581 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 8641 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 8701 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 8761 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 8821 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 8881 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 8941 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 9001 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 9061 ACGGATCGGG AGATCTCCCG ATCCCCTATG GT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.