pMSGV-1D3-G63811-P2A-Icam1 pla

pMSGV-1D3-G63811-P2A-Icam1 plasmid

PVT95578

pMSGV-1D3-G63811-P2A-Icam1 plasmid Information

Plasmid name: pMSGV-1D3-G63811-P2A-Icam1(mouse)
Alias: NM_010493.3;CD54; Icam-1; Ly-47; MALA-2
Gene lenght: 1614bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter:
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer:
Copy number: High
Induction:

pMSGV-1D3-G63811-P2A-Icam1 plasmid Description

pMSGV-1D3-G63811-P2A-Icam1 is a plasmid suitable for use in mammalian cells, and can also be used for retrovirus packaging.

pMSGV-1D3-G63811-P2A-Icam1 plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA GGTTAGGAAC AGAGAGACAG 121 CAGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA 181 GAACAGATGG TCCCCAGATG CGGTCCCGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT 241 TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT 301 TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC 361 CCCTCACTCG GCGCGCCAGT CCTCCGATAG ACTGCGTCGC CCGGGTACCC GTATTCCCAA 421 TAAAGCCTCT TGCTGTTTGC ATCCGAATCG TGGACTCGCT GATCCTTGGG AGGGTCTCCT 481 CAGATTGATT GACTGCCCAC CTCGGGGGTC TTTCATTTGG AGGTTCCACC GAGATTTGGA 541 GACCCCTGCC CAGGGACCAC CGACCCCCCC GCCGGGAGGT AAGCTGGCCA GCGGTCGTTT 601 CGTGTCTGTC TCTGTCTTTG TGCGTGTTTG TGCCGGCATC TAATGTTTGC GCCTGCGTCT 661 GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCGG 721 AACACCCGGC CGCAACCCTG GGAGACGTCC CAGGGACTTC GGGGGCCGTT TTTGTGGCCC 781 GACCTGAGTC CTAAAATCCC GATCGTTTAG GACTCTTTGG TGCACCCCCC TTAGAGGAGG 841 GATATGTGGT TCTGGTAGGA GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT 901 TGCTTTCGGT TTGGGACCGA AGCCGCGCCG CGCGTCTTGT CTGCTGCAGC ATCGTTCTGT 961 GTTGTCTCTG TCTGACTGTG TTTCTGTATT TGTCTGAAAA TATGGGCCCG GGCTAGCCTG 1021 TTACCACTCC CTTAAGTTTG ACCTTAGGTC ACTGGAAAGA TGTCGAGCGG ATCGCTCACA 1081 ACCAGTCGGT AGATGTCAAG AAGAGACGTT GGGTTACCTT CTGCTCTGCA GAATGGCCAA 1141 CCTTTAACGT CGGATGGCCG CGAGACGGCA CCTTTAACCG AGACCTCATC ACCCAGGTTA 1201 AGATCAAGGT CTTTTCACCT GGCCCGCATG GACACCCAGA CCAGGTCCCC TACATCGTGA 1261 CCTGGGAAGC CTTGGCTTTT GACCCCCCTC CCTGGGTCAA GCCCTTTGTA CACCCTAAGC 1321 CTCCGCCTCC TCTTCCTCCA TCCGCCCCGT CTCTCCCCCT TGAACCTCCT CGTTCGACCC 1381 CGCCTCGATC CTCCCTTTAT CCAGCCCTCA CTCCTTCTCT AGGCGCCCCC ATATGGCCAT 1441 ATGAGATCTT ATATGGGGCA CCCCCGCCCC TTGTAAACTT CCCTGACCCT GACATGACAA 1501 GAGTTACTAA CAGCCCCTCT CTCCAAGCTC ACTTACAGGC TCTCTACTTA GTCCAGCACG 1561 AAGTCTGGAG ACCTCTGGCG GCAGCCTACC AAGAACAACT GGACCGACCG GTGGTACCTC 1621 ACCCTTACCG AGTCGGCGAC ACAGTGTGGG TCCGCCGACA CCAGACTAAG AACCTAGAAC 1681 CTCGCTGGAA AGGACCTTAC ACAGTCCTGC TGACCACCCC CACCGCCCTC AAAGTAGACG 1741 GCATCGCAGC TTGGATACAC GCCGCCCACG TGAAGGCTGC CGACCCCGGG GGTGGACCAT 1801 CCTCTAGCCC TCGAGAAGCT TGCCACCatg gcctcaccgt tgacccgctt tctgtcgctg 1861 aacctgctgc tgctgggtga gtcgattatc ctggggagtg gagaagctCA GGTTCAGCTG 1921 AAGCAGTCTG GCCCAGGTCT GGTGCAGCCA TCACAGTCTC TGTCCATTAC CTGTACCGTG 1981 TCTGGCTTCT CACTGACCAA CTATGGCGTG CACTGGGTGA GACAGTCTCC AGGCAAAGGC 2041 CTGGAGTGGC TGGGCGTGAT CTGGTCAGGA GGCAACACCG ACTATAACAC CCCATTCACC 2101 TCAAGACTGT CTATCAACAA AGACAACTCA AAGTCTCAGG TGTTCTTCAA GATGAACTCT 2161 CTGCAGTCAA ACGACACCGC CATCTACTAT TGTGCCAGAG CTCTGACCTA CTATGACTAT 2221 GAGTTCGCCT ATTGGGGTCA AGGAACCCTG GTCACCGTCT CTGCTTCCTC TGGTGGCGGT 2281 GGCAGCGGCG GTGGTGGGAG TGGTGGCGGT GGCAGCGATA TCCTACTGAC CCAGTCCCCG 2341 GTGATCCTGT CCGTCTCTCC GGGAGAGAGG GTGTCCTTCT CCTGTCGTGC CAGTCAGAGC 2401 ATCGGGACGA ACATTCACTG GTATCAACAG CGCACTAATG GTTCTCCGCG TTTGCTGATT 2461 AAATATGCCT CTGAGAGCAT CTCTGGAatt ccaTCTCGCT TCTCTGGATC TGGGTCTGGA 2521 ACGGATTTCA CTCTGAGCAT CAACAGCGTA GAGTCTgaag acattGCCGA TTATTACTGT 2581 CAGCAAAATA ACAACTGGCC TACGACGTTC GGAgcaggaA CCAAGCTGGA ACTGAAATAC 2641 CCATACGACG TCCCAGACTA CGCAGAACAA AAACTCATCT CAGAAGAGGA TCTGgtgcca 2701 gtccttcaga aagtgaactc tactactacc aagccagtgc tgcgaactcc ctcacctgtg 2761 caccctaccg ggacatctca gccccagaga ccagaagatt gtcggccccg tggctcagtg 2821 aaggggaccg gattggactt cgcctgtgat atttacatct gggcaccctt ggccggaatc 2881 tgcgtggccc ttctgctgtc cttgatcatc actctcatct gctaccacag gagcaggaaa 2941 aaattccccc acatattcaa gcaaccattt aagaagacca ctggagcagc tcaagaggaa 3001 gatgcttgta gctgccgatg tccacaggaa gaagaaggag gaggaggagg ctatgagctg 3061 gcaaaattca gcaggagtgc agagactgct gccaacctgc aggaccccaa ccagctctac 3121 aatgagctca atctagggcg aagagaggaa tatgacgtct tggagaagaa gcgggctcgg 3181 gatccagaga tgggaggcaa acagcagagg aggaggaacc cccaggaagg cgtatacaat 3241 gcactgcaga aagacaagat ggcagaagcc tacagtgaga tcggcacaaa aggcgagagg 3301 cggagaggca aggggcacga tggcctttac cagggtctca gcactgccac caaggacacc 3361 tatgatgccc tgcaCatgca gaccctggcT cctAGGCATA TGACTAGTGT CGACGCCACT 3421 AACTTCTCCC TGTTGAAACA AGCAGGGGAT GTCGAAGAGA ATCCCGGGCC ACCGGTCGCC 3481 ACCatggctt caacccgtgc caagcccacg ctacctctgc tcctggccct ggtcaccgtt 3541 gtgatccctg ggcctggtga tgctcaggta tccatccatc ccagagaagc cttcctgccc 3601 cagggtgggt ccgtgcaggt gaactgttct tcctcatgca aggaggacct cagcctgggc 3661 ttggagactc agtggctgaa agatgagctc gagagtggac ccaactggaa gctgtttgag 3721 ctgagcgaga tcggggagga cagcagtccg ctgtgctttg agaactgtgg caccgtgcag 3781 tcgtccgctt ccgctaccat caccgtgtat tcgtttccgg agagtgtgga gctgagacct 3841 ctgccagcct ggcagcaagt aggcaaggac ctcaccctgc gctgccacgt ggatggtgga 3901 gcaccgcgga cccagctctc agcagtgctg ctccgtgggg aggagatact gagccgccag 3961 ccagtgggtg ggcaccccaa ggaccccaag gagatcacat tcacggtgct ggctagcaga 4021 ggggaccacg gagccaattt ctcatgccgc acagaactgg atctcaggcc gcaagggctg 4081 gcattgttct ctaatgtctc cgaggccagg agcctccgga ctttcgatct tccagctacc 4141 atcccaaagc tcgacacccc tgacctcctg gaggtgggca cccagcagaa gttgttttgc 4201 tccctggaag gcctgtttcc tgcctctgaa gctcggatat acctggagct gggaggccag 4261 atgccgaccc aggagagcac aaacagcagt gactctgtgt cagccactgc cttggtagag 4321 gtgactgagg agttcgacag aaccctgccg ctgcgctgcg ttttggagct agcggaccag 4381 atcctggaga cgcagaggac cttaacagtc tacaactttt cagctccggt cctgaccctg 4441 agccagctgg aggtctcgga agggagccaa gtaactgtga agtgtgaagc ccacagtggg 4501 tcgaaggtgg ttcttctgag cggcgtcgag cctaggccac ccaccccgca ggtccaattc 4561 acactgaatg ccagctcgga ggatcacaaa cgaagcttct tttgctctgc cgctctggag 4621 gtggcgggaa agttcctgtt taaaaaccag accctggaac tgcacgtgct gtatggtcct 4681 cggctggacg agacggactg cttggggaac tggacctggc aagaggggtc tcagcagact 4741 ctgaaatgcc aggcctgggg gaacccatct cctaagatga cctgcagacg gaaggcagat 4801 ggtgccctgc tgcccatcgg ggtggtgaag tctgtcaaac aggagatgaa tggtacatac 4861 gtgtgccatg cctttagctc ccatgggaat gtcaccagga atgtgtacct gacagtactg 4921 taccactctc aaaataactg gactataatc attctggtgc cagtactgct ggtcattgtg 4981 ggcctcgtga tggcagcctc ttatgtttat aaccgccaga gaaagatcag gatatacaag 5041 ttacagaagg ctcaggagga ggccataaaa ctcaagggac aagccccacc tccctgaCCG 5101 CGGGCCCGGG ATCCGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG 5161 AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG CAAGGCATGG 5221 AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG AGACAGCAGA 5281 ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC 5341 AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA GAGAACCATC AGATGTTTCC 5401 AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC 5461 TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT 5521 CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG GTACCCGTGT ATCCAATAAA 5581 CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG 5641 TGATTGACTA CCCGTCAGCG GGGGTCTTTC ATGGGTAACA GTTTCTTGAA GTTGGAGAAC 5701 AACATTCTGA GGGTAGGAGT CGAATATTAA GTAATCCTGA CTCAATTAGC CACTGTTTTG 5761 AATCCACATA CTCCAATACT CCTGAAATCC ATCGATGGAG TTCATTATGG ACAGCGCAGA 5821 AAGAGCTGGG GAGAATTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC 5881 GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG 5941 TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC 6001 GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT 6061 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 6121 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 6181 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 6241 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 6301 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 6361 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 6421 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 6481 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 6541 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 6601 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 6661 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 6721 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 6781 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 6841 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 6901 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 6961 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 7021 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 7081 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT 7141 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 7201 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 7261 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 7321 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 7381 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 7441 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 7501 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 7561 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 7621 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 7681 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 7741 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 7801 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 7861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 7921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA 7981 GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT 8041 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 8101 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 8161 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 8221 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC 8281 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA 8341 TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG 8401 TTTTCCCAGT CACGACGTTG TAAAACGACG GCGCAAGGAA TGGTGCATGC AAGGAGATGG 8461 CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA 8521 TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG 8581 CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGCGATTAG 8641 TCCAATTTGT TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC 8701 ACCAGCTGAA GCCTATAGAG TACGAGCCAT AGATAAAATA AAAGATTTTA TTTAGTCTCC 8761 AGAAAAAGGG GGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.