pMSGV-Icam1-P2A-EGFP plasmid

pMSGV-Icam1-P2A-EGFP plasmid

PVT89077

pMSGV-Icam1-P2A-EGFP plasmid Information

Plasmid name: pMSGV-Icam1(mouse)-P2A-EGFP
Alias: NM_010493.3;CD54; Icam-1; Ly-47; MALA-2
Gene lenght: 1614bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PEGFP-N-3
Copy number: High
Induction:

pMSGV-Icam1-P2A-EGFP plasmid Description

pMSGV-Icam1-P2A-EGFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMSGV-Icam1-P2A-EGFP plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA GGTTAGGAAC AGAGAGACAG 121 CAGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA 181 GAACAGATGG TCCCCAGATG CGGTCCCGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT 241 TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT 301 TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC 361 CCCTCACTCG GCGCGCCAGT CCTCCGATAG ACTGCGTCGC CCGGGTACCC GTATTCCCAA 421 TAAAGCCTCT TGCTGTTTGC ATCCGAATCG TGGACTCGCT GATCCTTGGG AGGGTCTCCT 481 CAGATTGATT GACTGCCCAC CTCGGGGGTC TTTCATTTGG AGGTTCCACC GAGATTTGGA 541 GACCCCTGCC CAGGGACCAC CGACCCCCCC GCCGGGAGGT AAGCTGGCCA GCGGTCGTTT 601 CGTGTCTGTC TCTGTCTTTG TGCGTGTTTG TGCCGGCATC TAATGTTTGC GCCTGCGTCT 661 GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCGG 721 AACACCCGGC CGCAACCCTG GGAGACGTCC CAGGGACTTC GGGGGCCGTT TTTGTGGCCC 781 GACCTGAGTC CTAAAATCCC GATCGTTTAG GACTCTTTGG TGCACCCCCC TTAGAGGAGG 841 GATATGTGGT TCTGGTAGGA GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT 901 TGCTTTCGGT TTGGGACCGA AGCCGCGCCG CGCGTCTTGT CTGCTGCAGC ATCGTTCTGT 961 GTTGTCTCTG TCTGACTGTG TTTCTGTATT TGTCTGAAAA TATGGGCCCG GGCTAGCCTG 1021 TTACCACTCC CTTAAGTTTG ACCTTAGGTC ACTGGAAAGA TGTCGAGCGG ATCGCTCACA 1081 ACCAGTCGGT AGATGTCAAG AAGAGACGTT GGGTTACCTT CTGCTCTGCA GAATGGCCAA 1141 CCTTTAACGT CGGATGGCCG CGAGACGGCA CCTTTAACCG AGACCTCATC ACCCAGGTTA 1201 AGATCAAGGT CTTTTCACCT GGCCCGCATG GACACCCAGA CCAGGTCCCC TACATCGTGA 1261 CCTGGGAAGC CTTGGCTTTT GACCCCCCTC CCTGGGTCAA GCCCTTTGTA CACCCTAAGC 1321 CTCCGCCTCC TCTTCCTCCA TCCGCCCCGT CTCTCCCCCT TGAACCTCCT CGTTCGACCC 1381 CGCCTCGATC CTCCCTTTAT CCAGCCCTCA CTCCTTCTCT AGGCGCCCCC ATATGGCCAT 1441 ATGAGATCTT ATATGGGGCA CCCCCGCCCC TTGTAAACTT CCCTGACCCT GACATGACAA 1501 GAGTTACTAA CAGCCCCTCT CTCCAAGCTC ACTTACAGGC TCTCTACTTA GTCCAGCACG 1561 AAGTCTGGAG ACCTCTGGCG GCAGCCTACC AAGAACAACT GGACCGACCG GTGGTACCTC 1621 ACCCTTACCG AGTCGGCGAC ACAGTGTGGG TCCGCCGACA CCAGACTAAG AACCTAGAAC 1681 CTCGCTGGAA AGGACCTTAC ACAGTCCTGC TGACCACCCC CACCGCCCTC AAAGTAGACG 1741 GCATCGCAGC TTGGATACAC GCCGCCCACG TGAAGGCTGC CGACCCCGGG GGTGGACCAT 1801 CCTCTAGCCC TCGAGgccac catggcttca acccgtgcca agcccacgct acctctgctc 1861 ctggccctgg tcaccgttgt gatccctggg cctggtgatg ctcaggtatc catccatccc 1921 agagaagcct tcctgcccca gggtgggtcc gtgcaggtga actgttcttc ctcatgcaag 1981 gaggacctca gcctgggctt ggagactcag tggctgaaag atgagctcga gagtggaccc 2041 aactggaagc tgtttgagct gagcgagatc ggggaggaca gcagtccgct gtgctttgag 2101 aactgtggca ccgtgcagtc gtccgcttcc gctaccatca ccgtgtattc gtttccggag 2161 agtgtggagc tgagacctct gccagcctgg cagcaagtag gcaaggacct caccctgcgc 2221 tgccacgtgg atggtggagc accgcggacc cagctctcag cagtgctgct ccgtggggag 2281 gagatactga gccgccagcc agtgggtggg caccccaagg accccaagga gatcacattc 2341 acggtgctgg ctagcagagg ggaccacgga gccaatttct catgccgcac agaactggat 2401 ctcaggccgc aagggctggc attgttctct aatgtctccg aggccaggag cctccggact 2461 ttcgatcttc cagctaccat cccaaagctc gacacccctg acctcctgga ggtgggcacc 2521 cagcagaagt tgttttgctc cctggaaggc ctgtttcctg cctctgaagc tcggatatac 2581 ctggagctgg gaggccagat gccgacccag gagagcacaa acagcagtga ctctgtgtca 2641 gccactgcct tggtagaggt gactgaggag ttcgacagaa ccctgccgct gcgctgcgtt 2701 ttggagctag cggaccagat cctggagacg cagaggacct taacagtcta caacttttca 2761 gctccggtcc tgaccctgag ccagctggag gtctcggaag ggagccaagt aactgtgaag 2821 tgtgaagccc acagtgggtc gaaggtggtt cttctgagcg gcgtcgagcc taggccaccc 2881 accccgcagg tccaattcac actgaatgcc agctcggagg atcacaaacg aagcttcttt 2941 tgctctgccg ctctggaggt ggcgggaaag ttcctgttta aaaaccagac cctggaactg 3001 cacgtgctgt atggtcctcg gctggacgag acggactgct tggggaactg gacctggcaa 3061 gaggggtctc agcagactct gaaatgccag gcctggggga acccatctcc taagatgacc 3121 tgcagacgga aggcagatgg tgccctgctg cccatcgggg tggtgaagtc tgtcaaacag 3181 gagatgaatg gtacatacgt gtgccatgcc tttagctccc atgggaatgt caccaggaat 3241 gtgtacctga cagtactgta ccactctcaa aataactgga ctataatcat tctggtgcca 3301 gtactgctgg tcattgtggg cctcgtgatg gcagcctctt atgtttataa ccgccagaga 3361 aagatcagga tatacaagtt acagaaggct caggaggagg ccataaaact caagggacaa 3421 gccccacctc ccGTCGACGC CACTAACTTC TCCCTGTTGA AACAAGCAGG GGATGTCGAA 3481 GAGAATCCCG GGCCACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG 3541 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC 3601 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC 3661 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC 3721 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA 3781 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC 3841 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC 3901 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC 3961 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC 4021 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC 4081 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC 4141 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT 4201 CTCGGCATGG ACGAGCTGTA CAAGTAACCG CGGGCCCGGG ATCCGATAAA ATAAAAGATT 4261 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA 4321 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAATACATA ACTGAGAATA GAGAAGTTCA 4381 GATCAAGGTT AGGAACAGAG AGACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG 4441 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA 4501 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG 4561 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC 4621 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG 4681 CGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC 4741 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC 4801 ATGGGTAACA GTTTCTTGAA GTTGGAGAAC AACATTCTGA GGGTAGGAGT CGAATATTAA 4861 GTAATCCTGA CTCAATTAGC CACTGTTTTG AATCCACATA CTCCAATACT CCTGAAATCC 4921 ATCGATGGAG TTCATTATGG ACAGCGCAGA AAGAGCTGGG GAGAATTGTG AAATTGTTAT 4981 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC 5041 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA 5101 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 5161 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 5221 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 5281 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 5341 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 5401 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 5461 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 5521 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 5581 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 5641 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 5701 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 5761 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 5821 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 5881 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 5941 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 6001 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 6061 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 6121 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 6181 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 6241 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 6301 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 6361 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 6421 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 6481 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 6541 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 6601 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 6661 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 6721 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 6781 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 6841 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 6901 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 6961 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 7021 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 7081 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT 7141 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC 7201 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC 7261 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT 7321 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA 7381 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG 7441 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT 7501 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG 7561 GCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG 7621 CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC 7681 CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG 7741 CCACGATGCG TCCGGCGTAG AGGCGATTAG TCCAATTTGT TAAAGACAGG ATATCAGTGG 7801 TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA GCCTATAGAG TACGAGCCAT 7861 AGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.