pLV2-CMV-PLAU-Neo plasmid

pLV2-CMV-PLAU-Neo plasmid

PVT95478

pLV2-CMV-PLAU-Neo plasmid Information

Plasmid name: pLV2-CMV-PLAU(human)-Neo
Alias: NM_002658.6;ATF; BDPLT5; QPD; u-PA; UPA; URK
Gene lenght: 1296bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-PLAU-Neo plasmid Description

pLV2-CMV-PLAU-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-PLAU-Neo plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcag tcatacggat 2821 CTCGAGGCCA CCatgagagc cctgctggcg cgcctgcttc tctgcgtcct ggtcgtgagc 2881 gactccaaag gcagcaatga acttcatcaa gttccatcga actgtgactg tctaaatgga 2941 ggaacatgtg tgtccaacaa gtacttctcc aacattcact ggtgcaactg cccaaagaaa 3001 ttcggagggc agcactgtga aatagataag tcaaaaacct gctatgaggg gaatggtcac 3061 ttttaccgag gaaaggccag cactgacacc atgggccggc cctgcctgcc ctggaactct 3121 gccactgtcc ttcagcaaac gtaccatgcc cacagatctg atgctcttca gctgggcctg 3181 gggaaacata attactgcag gaacccagac aaccggaggc gaccctggtg ctatgtgcag 3241 gtgggcctaa agctgcttgt ccaagagtgc atggtgcatg actgcgcaga tggaaaaaag 3301 ccctcctctc ctccagaaga attaaaattt cagtgtggcc aaaagactct gaggccccgc 3361 tttaagatta ttgggggaga attcaccacc atcgagaacc agccctggtt tgcggccatc 3421 tacaggaggc accggggggg ctctgtcacc tacgtgtgtg gaggcagcct catcagccct 3481 tgctgggtga tcagcgccac acactgcttc attgattacc caaagaagga ggactacatc 3541 gtctacctgg gtcgctcaag gcttaactcc aacacgcaag gggagatgaa gtttgaggtg 3601 gaaaacctca tcctacacaa ggactacagc gctgacacgc ttgctcacca caacgacatt 3661 gccttgctga agatccgttc caaggagggc aggtgtgcgc agccatcccg gactatacag 3721 accatctgcc tgccctcgat gtataacgat ccccagtttg gcacaagctg tgagatcact 3781 ggctttggaa aagagaattc taccgactat ctctatccgg agcagctgaa aatgactgtt 3841 gtgaagctga tttcccaccg ggagtgtcag cagccccact actacggctc tgaagtcacc 3901 accaaaatgc tgtgtgctgc tgacccacag tggaaaacag attcctgcca gggagactca 3961 gggggacccc tcgtctgttc cctccaaggc cgcatgactt tgactggaat tgtgagctgg 4021 ggccgtggat gtgccctgaa ggacaagcca ggcgtctaca cgagagtctc acacttctta 4081 ccctggatcc gcagtcacac caaggaagag aatggcctgg ccctctgagc ggccgcggat 4141 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 4201 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 4261 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 4321 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 4381 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 4441 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 4501 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 4561 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 4621 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca 4681 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc atggctgaac 4741 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 4801 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 4861 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 4921 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 4981 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 5041 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 5101 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 5161 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 5221 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 5281 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 5341 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 5401 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 5461 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 5521 tctgaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5581 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5641 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5701 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5761 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5821 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5881 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 5941 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6001 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6061 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 6121 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta 6181 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg 6241 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag 6301 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa 6361 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga 6421 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 6481 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 6541 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg 6601 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 6661 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 6721 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc 6781 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6841 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6901 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6961 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 7021 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc 7081 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 7141 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 7201 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 7261 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 7321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 7381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 7441 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 7501 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 7561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 7621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 7681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 7741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 7981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 8041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 8101 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 8161 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 8221 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 8281 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 8341 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 8401 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 8461 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 8521 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 8581 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 8641 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 8701 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 8761 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 8821 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 8881 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 8941 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 9001 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 9061 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 9121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 9181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 9241 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 9301 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 9361 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 9421 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg 9481 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat 9541 ttgttaaata tgtactacaa acttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.