pLV3-EF1a-MCS-HA-PGK-BSD plasm

pLV3-EF1a-MCS-HA-PGK-BSD plasmid

PVT91208

pLV3-EF1a-MCS-HA-PGK-BSD plasmid Information

Plasmid name: pLV3-EF1a-MCS-HA-PGK-BSD
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pLV3-EF1a-MCS-HA-PGK-BSD plasmid Description

pLV3-EF1a-MCS-HA-PGK-BSD It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-MCS-HA-PGK-BSD plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgaaggatcc gcggccgcTA TCCTTATGAT 2521 GTCCCAGACT ACGCATAGtg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc 2581 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat 2641 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct 2701 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg 2761 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa 2821 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg 2881 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg 2941 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct 3001 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttaccA 3061 TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA 3121 ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC 3181 GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG 3241 TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA 3301 ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC 3361 ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC 3421 GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAgt cgacaatcaa cctctggatt 3481 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 3541 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 3601 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 3661 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 3721 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 3781 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 3841 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 3901 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 3961 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 4021 cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac 4081 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 4141 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 4201 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 4261 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 4321 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 4381 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 4441 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4501 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4561 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 4621 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 4681 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat 4741 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 4861 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 4921 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 4981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5581 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6901 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 6961 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 7021 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 7081 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 7141 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 7201 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 7261 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 7321 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.