pCMV-USP28-3×Myc-Neo plasmid

pCMV-USP28-3×Myc-Neo plasmid

PVT95386

pCMV-USP28-3×Myc-Neo plasmid Information

Plasmid name: pCMV-USP28(human)-3×Myc-Neo
Alias: NM_020886.4;
Gene lenght: 3231bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-USP28-3×Myc-Neo plasmid Description

pCMV-USP28-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USP28-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgac tgcggagctg 721 cagcaggacg acgcggccgg cgcggcagac ggccacggct cgagctgcca aatgctgtta 781 aatcaactga gagaaatcac aggcattcag gacccttcct ttctccatga agctctgaag 841 gccagtaatg gtgacattac tcaggcagtc agccttctca ctgatgagag agttaaggag 901 cccagtcaag acactgttgc tacagaacca tctgaagtag aggggagtgc tgccaacaag 961 gaagtattag caaaagttat agaccttact catgataaca aagatgatct tcaggctgcc 1021 attgctttga gtctactgga gtctcccaaa attcaagctg atggaagaga tcttaacagg 1081 atgcatgaag caacctctgc agaaactaaa cgctcaaaga gaaaacgctg tgaagtctgg 1141 ggagaaaacc ccaatcccaa tgactggagg agagttgatg gttggccagt tgggctgaaa 1201 aatgttggca atacatgttg gtttagtgct gttattcagt ctctctttca attgcctgaa 1261 tttcgaagac ttgttctcag ttatagtctg ccacaaaatg tacttgaaaa ttgtcgaagt 1321 catacagaaa agagaaatat catgtttatg caagagcttc agtatttgtt tgctctaatg 1381 atgggatcaa atagaaaatt tgtagacccg tctgcagccc tggatctatt aaagggagca 1441 ttccgatcat ctgaggaaca gcagcaagat gtgagtgaat tcacacacaa gctcctggat 1501 tggctagagg acgcattcca gctagctgtt aatgttaaca gtcccaggaa caaatctgaa 1561 aatccaatgg tgcagctgtt ctatggtact ttcctgactg aaggggttcg tgaaggaaaa 1621 cccttttgta acaatgagac cttcggccag tatcctcttc aggtaaacgg ttatcgcaac 1681 ttagacgagt gtttggaagg ggccatggtg gagggtgatg ttgagcttct tccctccgat 1741 cactcggtga agtatggaca agagcgttgg tttacaaagc tacctccagt gttgaccttt 1801 gaactctcaa gatttgagtt taatcagtcc cttgggcagc cagagaaaat tcacaataag 1861 ctggaatttc ctcagattat ttatatggac aggtacatgt acaggagcaa ggagcttatt 1921 cgaaataaga gagagtgtat tcgaaagttg aaggaggaaa taaaaattct gcagcaaaaa 1981 ttggaaaggt atgtgaaata tggctcaggc ccagctcggt tcccgctccc ggacatgctg 2041 aaatatgtta ttgaatttgc tagtacaaaa cctgcctcag aaagctgtcc acctgaaagt 2101 gacacacata tgacattacc actttcttca gtgcactgct cggtttctga ccagacatcc 2161 aaggaaagta caagtacaga aagctcttct caggatgttg aaagtacctt ttcttctcct 2221 gaagattctt tacccaagtc taaaccactg acatcttctc ggtcttccat ggaaatgcct 2281 tcacagccag ctccacgaac agtcacagat gaggagataa attttgttaa gacctgtctt 2341 cagagatgga ggagtgagat tgaacaagat atacaagatt taaagacttg tattgcaagt 2401 actactcaga ctattgaaca gatgtactgc gatcctctcc ttcgtcaggt gccttatcgc 2461 ttgcatgcag ttcttgttca tgaaggacaa gcaaatgctg gacactattg ggcctatatc 2521 tataatcaac cccgacagag ctggctcaag tacaatgaca tctctgttac tgaatcttcc 2581 tgggaagaag ttgaaagaga ttcctatgga ggcctgagaa atgttagtgc ttactgtctg 2641 atgtacatta atgacaaact accctacttc aatgcagagg cagccccaac tgaatcagat 2701 caaatgtcag aagtggaagc cctatctgtg gaactcaagc attacattca ggaggataac 2761 tggcggtttg agcaggaagt agaggagtgg gaagaagagc agtcttgcaa aatccctcaa 2821 atggagtcct ccaccaactc ctcatcacag gactactcta catcacaaga gccttcagta 2881 gcctcttctc atggggttcg ctgcttgtcg tctgagcatg ctgtgattgt aaaggagcaa 2941 actgcccagg ctattgcaaa cacagcccgt gcctatgaga agagcggtgt agaagcggca 3001 ctgagtgagg tgatgctgag ccctgccatg caaggggtca tcctggccat agctaaagcc 3061 cgtcagacct ttgaccgaga tgggtctgaa gcagggctga ttaaggcatt ccatgaagaa 3121 tactccaggc tctatcagct tgccaaagag acccccacct ctcacagtga tcctcgactt 3181 cagcatgtcc ttgtctactt tttccaaaat gaagcaccca aaagggtagt agaacgaacc 3241 cttctggaac agtttgcaga taaaaatctt agctatgatg aaagatcaat cagcattatg 3301 aaggtggctc aagcgaaact gaaggaaatt ggtccagatg acatgaatat ggaagagtac 3361 aagaagtggc atgaagatta tagtttgttc cgaaaagtgt ctgtgtatct cctaacaggc 3421 ctagaactct atcaaaaagg aaagtaccaa gaggcacttt cctacctggt atatgcctac 3481 cagagcaatg ctgccctgct gatgaagggg ccccgccggg gggtcaaaga atccgtgatt 3541 gctttatacc gaagaaaatg ccttctggag ctgaatgcca aagcagcttc tctttttgaa 3601 acaaatgatg atcactccgt aactgagggc attaatgtga tgaatgaact gatcatcccc 3661 tgcattcacc ttatcattaa taatgacatt tccaaggatg atctggatgc cattgaggtc 3721 atgagaaacc attggtgctc ttaccttggg caagatattg cagaaaatct gcagctgtgc 3781 ctaggggagt ttctacccag acttctagat ccttctgcag aaatcatcgt cttgaaagag 3841 cctccaacta ttcgacccaa ttctccctat gacctatgta gccgatttgc agctgtcatg 3901 gagtcaattc agggagtttc aactgtgaca gtgaaaCTCG AGGAACAAAA ACTAATATCA 3961 GAGGAAGATT TGGAACAGAA ACTAATCAGT GAAGAAGATT TAGAGCAAAA GTTAATTTCC 4021 GAGGAGGACT TATAGTGAGG GCCCGATATC TCGACAATCA ACCTCTGGAT TACAAAATTT 4081 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 4141 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 4201 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 4261 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 4321 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 4381 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 4441 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 4501 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 4561 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 4621 TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG CTAACTGAAA CACGGAAGGA 4681 GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG 4741 GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA 4801 CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC 4861 CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA 4921 TAGCAGATCT GCGCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT 4981 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 5041 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 5101 AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 5161 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 5221 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 5281 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG GGGATTTCGG 5341 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA 5401 TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 5461 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 5521 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC 5581 CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 5641 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG 5701 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT 5761 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA 5821 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 5881 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT 5941 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC 6001 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG 6061 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC 6121 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 6181 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT 6241 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC 6301 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA 6361 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA 6421 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 6481 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC 6541 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT 6601 CTGGGGTTCG CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 6661 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 6721 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 6781 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 6841 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 6901 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 6961 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 7021 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 7081 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 7141 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 7201 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 7261 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 7321 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 7381 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 7441 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 7501 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT 7561 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 7621 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 7681 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 7741 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG 7801 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 7861 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 7921 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 7981 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 8041 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 8101 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 8161 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 8221 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 8281 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 8341 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 8401 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 8461 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 8521 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 8581 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 8641 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 8701 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 8761 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 8821 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 8881 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 8941 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 9001 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 9061 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC 9121 CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT 9181 CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG CTACAACAAG 9241 GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.