pLV2-Pcsk2-FLAG-Puro plasmid

pLV2-Pcsk2-FLAG-Puro plasmid

PVT95356

pLV2-Pcsk2-FLAG-Puro plasmid Information

Plasmid name: pLV2-Pcsk2(mouse)-FLAG-Puro
Alias: NM_008792.4;6330411F23Rik; Nec-2; Nec2; PC2; Phpp-2;SPC2
Gene lenght: 1911bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-Pcsk2-FLAG-Puro plasmid Description

pLV2-Pcsk2-FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-Pcsk2-FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgg agggcggttg tggatcccag tggaaggcgg ccgggttcct cttctgtgtg 2881 atggtttttg cgtctgccga gagacccgtc ttcacgaatc attttcttgt ggagttgcat 2941 aaagacggag aggaagaggc tcgccaagtt gcagcagaac acggctttgg agtccgaaag 3001 ctcccctttg cagaaggcct gtatcacttt tatcacaatg ggcttgcaaa ggccaaaaga 3061 agacgcagcc tacaccataa gcggcagcta gagagagacc ccaggataaa gatggcgctg 3121 caacaagaag gatttgaccg taaaaagaga gggtacaggg acatcaatga gattgacatc 3181 aacatgaatg atcctctctt tacaaagcaa tggtacctgt tcaacactgg gcaagccgat 3241 ggaactcctg ggctagactt gaacgtggcc gaagcctggg agctgggata cacaggaaaa 3301 ggagtgacca ttggaattat ggatgatgga attgactatc tccacccaga cctggcctac 3361 aactacaacg ctgatgcaag ttatgacttc agcagcaatg acccctaccc ataccctcga 3421 tacacagatg actggttcaa cagccatgga actaggtgtg caggagaagt ttctgctgca 3481 gccagcaaca atatctgtgg agtcggcgta gcatacaact ccaaggtggc agggatccgg 3541 atgctggacc agccctttat gacagacatc atcgaagcct cctccatcag ccacatgcct 3601 caactgatcg acatctacag tgcaagctgg ggccccacag acaatgggaa gacggttgat 3661 gggccccgag agctcacact ccaggccatg gctgatggcg tgaacaaggg ccgtgggggc 3721 aaaggcagca tctatgtgtg ggcctctggg gacggtggca gctacgatga ctgcaactgt 3781 gacggctatg cttcaagcat gtggaccatc tccatcaact cagccatcaa tgatggcagg 3841 actgccttgt atgatgagag ttgctcttcc accttagcat ccaccttcag caatgggagg 3901 aagaggaatc ctgaggctgg tgtggctacc acagacttgt atggcaactg tactctgaga 3961 cactctggga catctgcagc tgctccggag gcagctggcg tgtttgcatt agctttggag 4021 gctaacctgg atctgacctg gagagacatg caacatctga ctgtgctcac ctccaagcgg 4081 aaccagcttc atgatgaggt tcatcagtgg cgacggaatg gggttggcct ggaatttaat 4141 cacctctttg gctacggagt ccttgatgca ggtgccatgg tgaaaatggc taaagactgg 4201 aaaactgttc cggagagatt ccattgtgtg ggaggctctg tgcagaaccc tgaaaaaata 4261 ccacccaccg gcaagctggt actgaccctc aaaacaaatg catgtgaggg gaaggaaaac 4321 ttcgtccgct acctggagca cgtccaagct gtcatcacag tcaacgcgac caggagagga 4381 gacctgaaca tcaacatgac ctccccaatg ggcaccaagt ccattttgct gagccggcgt 4441 cccagagacg acgactccaa ggtgggcttt gacaagtggc ctttcatgac cacccacacc 4501 tggggggagg atgcccgagg gacctggacc ctggagctgg ggtttgtggg cagtgcacca 4561 cagaaggggt tgctgaagga atggaccctg atgcttcacg gcacacagag cgccccatac 4621 atcgatcagg tggtgaggga ttaccagtct aagctggcca tgtccaagaa gcaggagctg 4681 gaggaagagc tggatgaggc tgtggagaga agcctgcaaa gtatcctgag aaagaacgaa 4741 ttcGATTACA AGGATGACGA CGATAAGtga ggatcccgcg actctagata attctaccgg 4801 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 4861 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 4921 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 4981 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 5041 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 5101 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 5161 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 5221 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 5281 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg 5341 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 5401 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 5461 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 5521 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 5581 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 5641 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 5701 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 5761 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 5821 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 5881 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct 5941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6061 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6121 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6181 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6241 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6301 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 6361 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6421 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 6481 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc 6541 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 6601 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 6661 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga 6721 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 6781 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat 6841 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag 6901 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg 6961 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg 7021 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac 7081 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc 7141 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag 7201 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 7261 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 7321 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 7381 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat 7441 tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 7501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 8041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 8161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 8341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 8401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 9001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 9061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 9121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 9181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 9241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 9301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 9361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 9481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga 9661 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 9721 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 9781 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca 9841 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt 9901 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag 9961 tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.