pLV3-CMV-KAT2B-3×FLAG-Hyg plas

pLV3-CMV-KAT2B-3×FLAG-Hyg plasmid

PVT95320

pLV3-CMV-KAT2B-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-KAT2B(human)-3×FLAG-Hyg
Alias: NM_003884.5;CAF; P/CAF; PCAF
Gene lenght: 2496bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KAT2B-3×FLAG-Hyg plasmid Description

pLV3-CMV-KAT2B-3×FLAG-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-KAT2B-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatgtccga ggctggcggg gccgggccgg gcggctgcgg 2341 ggcaggagcc ggggcagggg ccgggcccgg ggcgctgccc ccgcagcctg cggcgcttcc 2401 gcccgcgccc ccgcagggct ccccctgcgc cgctgccgcc gggggctcgg gcgcctgcgg 2461 tccggcgacg gcagtggctg cagcgggcac ggccgaagga ccgggaggcg gtggctcggc 2521 ccgaatcgcc gtgaagaaag cgcaactacg ctccgctccg cgggccaaga aactggagaa 2581 actcggagtg tactccgcct gcaaggccga ggagtcttgt aaatgtaatg gctggaaaaa 2641 ccctaacccc tcacccactc cccccagagc cgacctgcag caaataattg tcagtctaac 2701 agaatcctgt cggagttgta gccatgccct agctgctcat gtttcccacc tggagaatgt 2761 gtcagaggaa gaaatgaaca gactcctggg aatagtattg gatgtggaat atctctttac 2821 ctgtgtccac aaggaagaag atgcagatac caaacaagtt tatttctatc tatttaagct 2881 cttgagaaag tctattttac aaagaggaaa acctgtggtt gaaggctctt tggaaaagaa 2941 acccccattt gaaaaaccta gcattgaaca gggtgtgaat aactttgtgc agtacaaatt 3001 tagtcacctg ccagcaaaag aaaggcaaac aatagttgag ttggcaaaaa tgttcctaaa 3061 ccgcatcaac tattggcatc tggaggcacc atctcaacga agactgcgat ctcccaatga 3121 tgatatttct ggatacaaag agaactacac aaggtggctg tgttactgca acgtgccaca 3181 gttctgcgac agtctacctc ggtacgaaac cacacaggtg tttgggagaa cattgcttcg 3241 ctcggtcttc actgttatga ggcgacaact cctggaacaa gcaagacagg aaaaagataa 3301 actgcctctt gaaaaacgaa ctctaatcct cactcatttc ccaaaatttc tgtccatgct 3361 agaagaagaa gtatatagtc aaaactctcc catctgggat caggattttc tctcagcctc 3421 ttccagaacc agccagctag gcatccaaac agttatcaat ccacctcctg tggctgggac 3481 aatttcatac aattcaacct catcttccct tgagcagcca aacgcaggga gcagcagtcc 3541 tgcctgcaaa gcctcttctg gacttgaggc aaacccagga gaaaagagga aaatgactga 3601 ttctcatgtt ctggaggagg ccaagaaacc ccgagttatg ggggatattc cgatggaatt 3661 aatcaacgag gttatgtcta ccatcacgga ccctgcagca atgcttggac cagagaccaa 3721 ttttctgtca gcacactcgg ccagggatga ggcggcaagg ttggaagagc gcaggggtgt 3781 aattgaattt cacgtggttg gcaattccct caaccagaaa ccaaacaaga agatcctgat 3841 gtggctggtt ggcctacaga acgttttctc ccaccagctg ccccgaatgc caaaagaata 3901 catcacacgg ctcgtctttg acccgaaaca caaaaccctt gctttaatta aagatggccg 3961 tgttattggt ggtatctgtt tccgtatgtt cccatctcaa ggattcacag agattgtctt 4021 ctgtgctgta acctcaaatg agcaagtcaa gggctatgga acacacctga tgaatcattt 4081 gaaagaatat cacataaagc atgacatcct gaacttcctc acatatgcag atgaatatgc 4141 aattggatac tttaagaaac agggtttctc caaagaaatt aaaataccta aaaccaaata 4201 tgttggctat atcaaggatt atgaaggagc cactttaatg ggatgtgagc taaatccacg 4261 gatcccgtac acagaatttt ctgtcatcat taaaaagcag aaggagataa ttaaaaaact 4321 gattgaaaga aaacaggcac aaattcgaaa agtttaccct ggactttcat gttttaaaga 4381 tggagttcga cagattccta tagaaagcat tcctggaatt agagagacag gctggaaacc 4441 gagtggaaaa gagaaaagta aagagcccag agaccctgac cagctttaca gcacgctcaa 4501 gagcatcctc cagcaggtga agagccatca aagcgcttgg cccttcatgg aacctgtgaa 4561 gagaacagaa gctccaggat attatgaagt tataaggttc cccatggatc tgaaaaccat 4621 gagtgaacgc ctcaagaata ggtactacgt gtctaagaaa ttattcatgg cagacttaca 4681 gcgagtcttt accaattgca aagagtacaa cccccctgag agtgaatact acaaatgtgc 4741 caatatcctg gagaaattct tcttcagtaa aattaaggaa gctggattaa ttgacaagCT 4801 CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 4861 CGATGACAAG TAGcggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 4921 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 4981 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 5041 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 5101 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 5161 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 5221 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 5281 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 5341 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 5401 agatccaagc tgtgaccggc gcctacgtcg acatgaaaaa gcctgaactc accgcgacgt 5461 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg 5521 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg 5581 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg 5641 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt 5701 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg 5761 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga 5821 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt 5881 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg 5941 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg 6001 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc 6061 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg 6121 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg 6181 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg 6241 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc 6301 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg 6361 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa 6421 accgacgccc cagcactcgt ccgagggcaa aggaataggt cgacaatcaa cctctggatt 6481 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6541 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6601 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6661 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6721 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6781 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6841 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 6901 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6961 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 7021 cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac 7081 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 7141 cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 7201 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 7261 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 7321 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 7381 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 7441 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 7501 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 7561 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 7621 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 7681 agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat 7741 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 7801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 7861 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 7921 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 7981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 8041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 8101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 8161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 8221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 8281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 8341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 8401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 8461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 8521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 8581 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 8641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 8701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 8761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 8821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 8881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 8941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 9001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 9061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 9121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 9181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 9241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 9301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 9361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 9421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 9481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 9541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 9601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 9661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 9721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 9781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 9841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 9901 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 9961 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 10021 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 10081 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 10141 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 10201 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 10261 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 10321 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.