pCMV-CSTF3-EGFP-3×FLAG-Neo pla

pCMV-CSTF3-EGFP-3×FLAG-Neo plasmid

PVT95275

pCMV-CSTF3-EGFP-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-CSTF3(human)-EGFP-3×FLAG-Neo
Alias:
Gene lenght: 2151bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CSTF3-EGFP-3×FLAG-Neo plasmid Description

pCMV-CSTF3-EGFP-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CSTF3-EGFP-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatgtcag gagacggagc cacggagcag gcagctgagt atgtcccaga gaaggtgaag 1021 aaagcggaaa agaaattaga agagaatcca tatgaccttg atgcttggag cattctcatt 1081 cgagaggcac agaatcaacc tatagacaaa gcacggaaga cttatgaacg ccttgttgcc 1141 cagttcccca gttctggcag attctggaaa ctgtacattg aagcagagat taaagctaaa 1201 aattatgaca aggttgaaaa gctatttcag agatgcctta tgaaggtttt gcacattgat 1261 ttatggaagt gttatctttc atatgtccga gaaaccaagg gtaaactacc aagttacaaa 1321 gaaaaaatgg ctcaagcata tgactttgca ctggataaaa ttggaatgga aattatgtcc 1381 tatcagattt gggtggatta catcaatttc ctaaaaggcg tggaagctgt aggatcttat 1441 gcagaaaatc aaagaataac agctgtccga agagtttatc aacgaggttg tgttaatccg 1501 atgatcaaca ttgaacagct ctggagagac tataacaagt atgaagaggg tatcaatatt 1561 catttagcta aaaaaatgat tgaagatcgg agtagagatt atatgaatgc tagacgtgta 1621 gcaaaggaat atgagacagt aatgaaaggc ttggaccgta atgctccctc ggtgcctcct 1681 cagaatactc ctcaagaagc tcaacaagta gatatgtgga agaaatatat acagtgggaa 1741 aagagcaacc ctcttcgtac agaggatcag acccttataa caaaaagagt tatgtttgct 1801 tatgaacagt gcctgcttgt gctgggccat caccctgata tttggtatga agctgcccag 1861 tatcttgagc agtcaagtaa actgctcgca gaaaagggag atatgaataa tgccaaatta 1921 tttagtgatg aagctgctaa tatatatgaa agagccataa gcactttatt gaagaagaat 1981 atgcttcttt attttgcata tgcagattat gaagagagtc gcatgaagta tgaaaaggtt 2041 cacagtatat ataacagact tctggcaatt gaggatattg accctacctt ggtatatatc 2101 caatatatga aatttgcacg gagagcagaa ggcatcaaat ctggaagaat gatatttaaa 2161 aaagcaagag aagataccag aacccgccac catgtctatg ttactgcagc actcatggaa 2221 tattactgta gtaaggacaa atctgttgcc tttaagattt ttgagctggg gctaaaaaaa 2281 tatggagaca ttccagagta tgtcctggcc tatattgact atctttctca cctcaatgag 2341 gacaataata cccgagtttt gtttgaacga gttttaacat ctggaagcct tcctcctgag 2401 aagtctggag aaatctgggc ccgatttcta gcatttgaaa gtaatattgg tgatctagct 2461 agtatactca aagtggagaa aagacggttt acagcattca aagaagagta tgaagggaaa 2521 gaaacggctt tactagtaga tagatacaag ttcatggatt tatatccttg ctctgcaagt 2581 gaattaaaag cacttggtta taaggatgtc tcccgtgcta agctagcagc tataattccg 2641 gacccagttg tagctccttc tatagtgcct gttctgaaag atgaagtgga tagaaaacca 2701 gaatacccta aaccagacac tcagcagatg attccatttc agccacgaca tttagcacct 2761 ccaggtttac accctgtacc tggtggagtg ttcccagtcc ctcctgcagc tgttgtttta 2821 atgaaacttc tccctcctcc tatctgtttc cagggtcctt ttgtacaagt ggatgaactg 2881 atggaaattt tccgaagatg caagatacca aatactgttg aggaagctgt gaggatcatt 2941 actggtgggg ccccagagct agctgtagaa ggcaacggcc ccgtggaaag taatgcagta 3001 ctcaccaagg ccgtcaaaag gcccaacgag gattcagatg aagatgaaga aaagggagcc 3061 gttgtccccc ctgttcatga catttacaga gcacggcagc agaagcggat tcggGGCGGT 3121 GGAGGAAGCg gcggaggtgg atctatggtg agcaagggcg aggagctgtt caccggggtg 3181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 3241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 3301 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 3361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 3421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 3481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 3541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 3601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 3661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 3721 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 3781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 3841 ggcatggacg agctgtacaa gCTCGAGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3901 CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGTTTA AACCCGCTGA 3961 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 4021 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA 4081 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG 4141 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT 4201 GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA 4261 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 4321 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 4381 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 4441 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 4501 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 4561 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 4621 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA 4681 ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 4741 GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 4801 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC 4861 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 4921 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG 4981 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT 5041 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 5101 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 5161 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 5221 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 5281 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 5341 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 5401 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 5461 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 5521 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 5581 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 5641 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 5701 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 5761 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 5821 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 5881 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 5941 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT 6001 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC 6061 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 6121 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT 6181 ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 6241 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 6301 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 6361 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 6421 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 6481 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 6541 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 6601 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 6661 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 6721 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 6781 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 6841 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 6901 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 6961 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 7021 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 7081 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 7141 CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 7201 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 7261 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 7321 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 7381 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 7441 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 7501 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 7561 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 7621 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 7681 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 7741 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 7801 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 7861 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 7921 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 7981 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 8041 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 8101 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 8161 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 8221 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 8281 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 8341 CACATTTCCC CGAAAAGTGC CACCTGACGT C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.