pLV3-U6-MIR124-3P-shRNA1-CopGF

pLV3-U6-MIR124-3P-shRNA1-CopGFP-Puro plasmid

PVT95245

pLV3-U6-MIR124-3P-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-MIR124(human)-3P-shRNA1-CopGFP-Puro
Alias: CGGTAACTTTGACCAGCCGA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-MIR124-3P-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-MIR124-3P-shRNA1-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-MIR124-3P-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg taaggcacgc ggtgaatgcc aattcaagag 2221 attggcattc accgcgtgcc ttattttttg gatccgcggc cgcGaaggat ctgcgatcgc 2281 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 2341 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 2401 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 2461 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 2521 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 2581 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 2641 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 2701 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 2761 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 2821 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 2881 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 2941 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 3001 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 3061 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 3121 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 3181 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 3241 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 3301 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 3361 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 3421 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 3481 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 3541 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 3601 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 3661 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 3721 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 3781 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 3841 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 3901 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 3961 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4021 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 4081 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 4141 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 4201 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 4261 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 4321 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 4381 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 4441 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 4501 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 4561 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 4621 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 4681 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 4741 ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc 4801 tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta 4861 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 4921 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 4981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5101 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5161 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 5341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 5461 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc 5521 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5581 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5641 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5701 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 5761 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 5821 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 5881 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 5941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 6361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6481 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6541 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6601 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 6661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 6781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 6841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 6901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 6961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 7681 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 7741 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 7801 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 7861 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 7921 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 7981 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 8041 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 8101 agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.